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Fine mapping of powdery mildew resistance gene MlWE74 derived from wild emmer wheat (Triticum turgidum ssp. dicoccoides) in an NBS-LRR gene cluster

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Abstract

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Powdery mildew resistance gene MlWE74, originated from wild emmer wheat accession G-748-M, was mapped in an NBS-LRR gene cluster of chromosome 2BS.

Abstract

Wheat powdery mildew, caused by Blumeria graminis f. sp. tritici (Bgt), is a globally devastating disease. Wild emmer wheat (Triticum turgidum var. dicoccoides) is a valuable genetic resource for improving disease resistance in common wheat. A powdery mildew resistance gene was transferred to hexaploid wheat line WE74 from wild emmer accession G-748-M. Genetic analysis revealed that the powdery mildew resistance in WE74 is controlled by a single dominant gene, herein temporarily designated MlWE74. Bulked segregant analysis (BSA) and molecular mapping delimited MlWE74 to the terminal region of chromosome 2BS flanking by markers WGGBD412 and WGGBH346 within a genetic interval of 0.25 cM and corresponding to 799.9 kb genomic region in the Zavitan reference sequence. Sequence annotation revealed two phosphoglycerate mutase-like genes, an alpha/beta-hydrolases gene, and five NBS-LRR disease resistance genes that could serve as candidates for map-based cloning of MlWE74. The geographical location analysis indicated that MlWE74 is mainly distributed in Rosh Pinna and Amirim regions, in the northern part of Israel, where environmental conditions are favorable to the occurrence of powdery mildew. Moreover, the co-segregated marker WGGBD425 is helpful in marker-assisted transfer of MlWE74 into elite cultivars.

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All data generated or analyzed during this study are included in this published article and its supplementary information files.

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Acknowledgements

We are grateful to Prof. Tsomin Yang and Qixin Sun for providing the wheat line WE74. This work was financially supported by the National Natural Science Foundation of China (32101735).

Funding

Young Scientists Fund, 32101735, Miaomiao Li.

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Authors and Affiliations

  1. State Key Laboratory of Plant Cell and Chromosome Engineering, Institute of Genetics and Developmental Biology, The Innovative Academy of Seed Design, Chinese Academy of Sciences, Beijing, 100101, China

    Keyu Zhu, Miaomiao Li, Lingli Dong, Qiuhong Wu, Yongxing Chen, Jingzhong Xie, Ping Lu, Guanghao Guo, Huaizhi Zhang, Panpan Zhang, Beibei Li, Wenling Li, Lei Dong & Zhiyong Liu

  2. University of Chinese Academy of Sciences, Beijing, 100049, China

    Keyu Zhu, Guanghao Guo, Huaizhi Zhang, Panpan Zhang, Beibei Li, Wenling Li, Lei Dong & Zhiyong Liu

  3. Academy of National Food and Strategic Reserves Administration, Beijing, 100037, China

    Haibin Wu

  4. Chaozhou Hybribio Biochemistry Ltd., Chaozhou, 521011, Guangdong, China

    Deyun Zhang

  5. Zhejiang Academy of Agricultural Sciences, Hangzhou, 310021, China

    Qifei Wang, Jinghuan Zhu & Wei Hua

  6. Hebei Gaoyi Stock Seed Farm, Gaoyi, 051330, Hebei, China

    Wenli Hu, Liqiao Guo, Rongge Wang & Chengguo Yuan

  7. The National Engineering Laboratory of Crop Molecular Breeding, Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing, 100081, China

    Hongjie Li

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  1. Keyu Zhu
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  2. Miaomiao Li
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Contributions

WH, ZYL and MML designed the experiments. KYZ, MML, HBW, DYZ, LLD, QHW, YXC, JZX, PL, GHG, HZZ, PPZ, BBL, WLL, LD, QFW, JHZ, WLH, LQG, RGW, and CGY performed the experiments, conducted fieldwork, analyzed data, and performed Bgt inoculation. KYZ, MML, WH, HJL and ZYL wrote the paper. All authors read, revised, and approved the manuscript.

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Correspondence to Miaomiao Li, Zhiyong Liu or Wei Hua.

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Zhu, K., Li, M., Wu, H. et al. Fine mapping of powdery mildew resistance gene MlWE74 derived from wild emmer wheat (Triticum turgidum ssp. dicoccoides) in an NBS-LRR gene cluster. Theor Appl Genet 135, 1235–1245 (2022). https://doi.org/10.1007/s00122-021-04027-2

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