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Marker assisted separation of resistance genes Rph22 and Rym16 Hb from an associated yield penalty in a barley: Hordeum bulbosum introgression line

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Abstract

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The resistance genes Rph22 and Rym16 Hb transferred into barley from Hordeum bulbosum have been separated from a large yield penalty locus that was present in the original introgression line ‘182Q20’.

Abstract

The Hordeum bulbosum introgression line ‘182Q20’ possesses resistance to barley leaf rust (Rph22) and Barley mild mosaic virus (Rym16 Hb) located on chromosome 2HL. Unfortunately, this line also carries a considerable yield penalty compared with its barley genetic background ‘Golden Promise’. Quantitative trait locus (QTL) mapping of the components of yield (total yield, thousand grain weight, hectolitre weight, percentage screenings and screened yield) was performed using 75 recombinant lines derived from the original ‘182Q20’ introgression line. A QTL for the yield penalty was located in the proximal region of the introgressed segment. Marker assisted selection targeting intraspecific recombination events between overlapping H. bulbosum introgression segments was used to develop the lines ‘372E’ and ‘372H’ which feature genetically small introgressions around Rph22. Further yield trials validated the separation of both Rph22 and Rym16 Hb from the proximal yield penalty. These results, combined with molecular markers closely linked to Rph22 and Rym16 Hb, make these resistance genes more attractive for barley breeding.

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Acknowledgments

The authors wish to acknowledge the efforts of Andy Hay, Russell Harrison-Kirk and their team for sowing, managing and harvesting the field trials, Dr. Soonie Chng for glasshouse leaf rust screening of line ‘372E’ and Donna Gibson for formatting of Fig. 1. Also thanks go to Dr Samantha Baldwin, Andy Hay and Dr. Bill Griffin for critical reading of this manuscript. Funding for this research was initially provided by the New Zealand Foundation for Research, Science and Technology contract number C06X0810 and more recently supported by Plant and Food Research Core funding.

Conflict of interest

The authors declare that they have no conflict of interest.

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Authors and Affiliations

  1. The New Zealand Institute for Plant and Food Research Limited, Lincoln, 7608, New Zealand

    Paul A. Johnston, Vijitha Meiyalaghan, Merle E. Forbes, Ruth C. Butler & Richard Pickering

  2. Julius Kühn-Institute, Institute for Resistance Research and Stress Tolerance, Erwin-Baur-Str. 27, 06484, Quedlinburg, Germany

    Antje Habekuß

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  1. Paul A. Johnston
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  2. Vijitha Meiyalaghan
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  3. Merle E. Forbes
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Correspondence to Paul A. Johnston.

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Communicated by T. Komatsuda.

R. Pickering: retired, formerly of The New Zealand Institute for Plant and Food Research Limited.

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Johnston, P.A., Meiyalaghan, V., Forbes, M.E. et al. Marker assisted separation of resistance genes Rph22 and Rym16 Hb from an associated yield penalty in a barley: Hordeum bulbosum introgression line. Theor Appl Genet 128, 1137–1149 (2015). https://doi.org/10.1007/s00122-015-2495-z

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