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Lr67/Yr46 confers adult plant resistance to stem rust and powdery mildew in wheat

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Abstract

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We demonstrate that Lr67/Yr46 has pleiotropic effect on stem rust and powdery mildew resistance and is associated with leaf tip necrosis. Genes are designated as Sr55, Pm46 and Ltn3 , respectively.

Abstract

Wheat (Triticum aestivum) accession RL6077, known to carry the pleiotropic slow rusting leaf and yellow rust resistance genes Lr67/Yr46 in Thatcher background, displayed significantly lower stem rust (P. graminis tritici; Pgt) and powdery mildew (Blumeria graminis tritici; Bgt) severities in Kenya and in Norway, respectively, compared to its recurrent parent Thatcher. We investigated the resistance of RL6077 to stem rust and powdery mildew using Avocet × RL6077 F6 recombinant inbred lines (RILs) derived from two photoperiod-insensitive F3 families segregating for Lr67/Yr46. Greenhouse seedling tests were conducted with Mexican Pgt race RTR. Field evaluations were conducted under artificially initiated stem rust epidemics with Pgt races RTR and TTKST (Ug99 + Sr24) at Ciudad Obregon (Mexico) and Njoro (Kenya) during 2010–2011; and under natural powdery mildew epiphytotic in Norway at Ås and Hamar during 2011 and 2012. In Mexico, a mean reduction of 41 % on stem rust severity was obtained for RILs carrying Lr67/Yr46, compared to RILs that lacked the gene, whereas in Kenya the difference was smaller (16 %) but significant. In Norway, leaf tip necrosis was associated with Lr67/Yr46 and RILs carrying Lr67/Yr46 showed a 20 % reduction in mean powdery mildew severity at both sites across the 2 years of evaluation. Our study demonstrates that Lr67/Yr46 confers partial resistance to stem rust and powdery mildew and is associated with leaf tip necrosis. The corresponding pleiotropic, or tightly linked, genes, designated as Sr55, Pm46, and Ltn3, can be utilized to provide broad-spectrum durable disease resistance in wheat.

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Acknowledgments

We acknowledge the financial support by the Australian Grains Research and Development Corporation (GRDC) and Australian Cereal Rust Control Program, and appreciate the technical editing by Emma Quilligan (CIMMYT). The field testing of powdery mildew resistance in Norway was supported by a Grant from the Research Council of Norway (NFR 185046).

Conflict of interest

The authors declare that they have no conflict of interest.

Ethical standard

The experiments comply with the current laws of the country in which they were performed.

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Authors and Affiliations

  1. International Maize and Wheat Improvement Center (CIMMYT), Apdo. Postal 6-641, 06600, México, D.F., Mexico

    Sybil A. Herrera-Foessel, Ravi P. Singh, Sridhar Bhavani, Sukhwinder Singh, Caixia Lan & Violeta Calvo-Salazar

  2. Department of Plant and Environmental Sciences, Norwegian University of Life Sciences, P.O. Box 5003, 1432, Ås, Norway

    Morten Lillemo

  3. Campo Experimental Valle de México INIFAP, Apdo. Postal 10, 56230, Chapingo, Edo. de México, Mexico

    Julio Huerta-Espino

  4. CSIRO Plant Industry, GPO Box 1600, Canberra, ACT, 2601, Australia

    Evans S. Lagudah

Authors
  1. Sybil A. Herrera-Foessel
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  2. Ravi P. Singh
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  3. Morten Lillemo
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  4. Julio Huerta-Espino
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  6. Sukhwinder Singh
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  7. Caixia Lan
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  8. Violeta Calvo-Salazar
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  9. Evans S. Lagudah
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Correspondence to Sybil A. Herrera-Foessel.

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Communicated by J. Yan.

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122_2013_2256_MOESM1_ESM.docx

Supplementary material 1 (DOCX 225 kb) Fig. S1 Infected stems with Pgt race RTR of two RILs from the Avocet/RL6077 population, not carrying Lr67/Yr46 (left) and carrying Lr67/Yr46 (right), when evaluated at Ciudad Obregon, Mexico, in the 2010-2011 crop cycle

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Herrera-Foessel, S.A., Singh, R.P., Lillemo, M. et al. Lr67/Yr46 confers adult plant resistance to stem rust and powdery mildew in wheat. Theor Appl Genet 127, 781–789 (2014). https://doi.org/10.1007/s00122-013-2256-9

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