Abstract
The increasing economic importance of triticale (×Triticosecale Wittm.) makes this synthetic hybrid cereal an interesting object of genetic studies. Genomic regions (QTL) of morphological winter triticale traits were determined using the mapping population of 89 doubled haploids lines (DHs) developed from F1 hybrid of cv. ‘Hewo’ and cv. ‘Magnat’ accompanied with the genetic map consisting of 20 linkage groups assigned to the A (7), B (7), and R (6) genomes (total of 3539 DArT, SNP-DArT and SSR markers, length of 4997.4 cM). Five independent experiments were performed in the field and greenhouse controlled conditions. A total of 12 major QTLs located on 2B, 5A, 5R, and 6B chromosomes connected to the stem length, the plant height, the spike length, the number of the productive spikelets per spike, the number of grains per spike, and the thousand kernel weight were identified by a composite interval mapping (CIM).
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Alheit, K.V., Busemayer, L., Liu, W, Maurer, H.P., Gowda, M., Hahn, V., Weissmann, S., Ruckelshausen, A., Reif, J.C., Würschum, T. 2014. Multiple-line cross QTL mapping for biomass yield and plant height in triticale (×Triticosecale Wittmack). Theor. Appl. Genet. 127:251–260.
Ammar, K., Mergoum, M,. Rajaram, S. 2004. The history and evolution of triticale. In: M. Mergoum (ed.) Triticale improvement and production. Fao Plant Production and Protection Paper 179.
Badea, A., Eudes, F., Salmon, D., Tuvesson, S., Vrolijk, A., Larsson, C.T., Caig, V., Huttner, E., Kilian, A., Laroche, A. 2011. Development and assessment of DArT markers in triticale. Theor. Appl. Genet. 122:1547–1560.
Banaszak, Z. 2011. Breeding of triticale in DANKO. Bericht über die 61:65–68.
Blum, A. 2014. The abiotic stress response and adaptation of triticale. Cereal Research Communications 42:359–375.
Börner, A., Korzun, A.V., Voylokov, A.V., Weberm, W.E. 1999. Detection of quantitative trait loci on chromosome 5R of rye (Secale cereale L.). Theor. Appl. Genet. 98:1087–1090.
Börner, A., Schumann, E., Furste, A., Coster, H., Leithold, B., Röder, M.S., Weber, W.E. 2002. Mapping of quantitative trait loci for agronomic important characters in hexaploid wheat (Triticum aestivum L.). Theor. Appl. Genet. 105:921–936.
Cui, F., Ding, A., Li, J., Zhao, C., Wang, L., Wang, X., Qi, X., Li, X., Li, G., Gao, J., Wang, H. 2012. QTL detection of seven spike-related traits and their genetic correlations in wheat using two related RIL populations. Euphytica 186:177–192.
Gao, F., Zeng, L., Qiu, L., Lu, X., Ren, J., Wu, X., Su, X., Gao, Y., Ren, G. 2016. QTL mapping of grain appearance quality traits and grain weight using a recombinant inbred population in rice (Oryza sativa L.). J. Integr. Agric. 15:1693–1702.
Gawroński, P., Pawełkowicz, M., Tofil, K., Uszyński, G., Sharifova, S., Ahluvalia, S., Tyrka, M., Wędzony, M., Kilian, A., Bolibok-Brągoszewska, H. 2016. DArT markers effectively target gene space in the rye genome. Frontiers in Plant Science 7:1–13.
Golba, J., Rozbicki, J., Gozdowski, D., Sas, D., Madry, W., Piechocinski, M., Kurzyńska, L. Studnicki, M., Derejko, A. 2013. Adjusting yield components under different levels of N applications in winter wheat. International Journal of Plant Production 7(1):139–150.
Gołębiowska, G., Wędzony, M. 2009. Cold-hardening of winter triticale (×Triticosecale Wittm.) results in increased resistance to pink snow mould Microdochium nivale (Fr., Samuels & Hallett) and genotype-dependent chlorophyll fluorescence modulations. Acta Phys. Plant. 31:12–19.
Hoagland, D.R., Arnon, D.I. 1938. A water culture method for growing plants without soil. Circ. Univ. Calif. Agric. Exp. Stn. No. 347.
Kato, K., Miura, H., Sawada, S. 2000. Mapping QTLs controlling grain yield and its components on chromosome 5A of wheat. Theor. Appl. Genet. 101:1114–1121.
Kalih, R., Maurer, H.P., Hackauf, B., Miedaner, T. 2014. Effect of a rye dwarfing gene on plant height, heading stage and Fusarium head blight in triticale (×Triticosecale Wittmack). Theor. Appl. Genet. 127:1527–1536.
Khalil, H.B., Ehdaeivand, M.R., Xu, Y., Laroche, A., Gulick, P.J. 2015. Identification and characterization of rye genes not expressed in allohexaploid triticale. BMC genomics, 16:281.
Korzun, V., Börner, A., Melz, G. 1996. RFLP mapping of the dwarfing (Ddw1) and hairy peduncle (Hp) genes on chromosome 5 of rye (Secale cereale L.). Theor. Appl. Genet. 92:1073–1077.
Kumar, N., Kulwal, P.L., Gaur, A., Tyagi, A.K., Khurana, J.P., Khurana, P., Balyan, H.S., Gupta, P.K. 2006. QTL analysis for grain weight in common wheat. Euphytica, 151(2):135–144.
Kumar, N., Kulwal, P.L., Balyan, H.S., Gupta, P.K. 2007. QTL mapping for yield and yield contributing traits in two mapping populations of bread wheat. Molecular Breeding 19(2):163–177.
Li, W.L., Nelson, J.C., Chu, C.Y., Shi, L.H., Huang, S.H., Liu, D.J. 2002. Chromosomal locations and genetic relationships of tiller and spike characters in wheat. Euphytica 125:357–366.
Liu, W., Leiser, W.L., Reif, J.C., Tucker, M.R., Losert, D., Weissmann, S., Hahn, V., Maurer, H.P., Würschum, T. 2016. Multiple-line cross QTL mapping for grain yield and thousand kernel weight in triticale. Plant Breeding 135:567–573.
Lukaszewski, A.J. 2003. Registration of three germplasms of hexaploid triticale with introgressions of wheat storage protein loci from chromosome 1D of bread wheat. Crop science 43:2316–2317.
Machczyńska, J., Orłowska, R., Mańkowski, D.R., Zimny, J., Bednarek, P.T. 2014. DNA methylation changes in triticale due to in vitro culture plant regeneration and consecutive reproduction. Plant Cell, Tissue and Organ Culture 119(2):289–299.
Malyshev, S.V., Kartel, N.A., Voylokov, A.V., Korzun, V., Börner, A. 2003. Comparative analysis of QTLs affecting agronomical traits in rye and wheat. In: Börner, A., Snape, J.W., Law, C.N. (eds) European Wheat Aneuploid Co-operative Newsletter. Proceedings of the 12th EWAC Conference. Ss., pp. 120–122.
Mangini, G., Gadaleta, A., Colasuonno, P., Marcotuli, I., Signorile, A.M., Simeone, R., De Vita, P., Mastrangelo, A.M., Laidò, G., Pecchioni, N., Blanco, A. 2018. Genetic dissection of the relationships between grain yield components by genome-wide association mapping in a collection of tetraploid wheats. PLoS ONE 13(1): e0190162.
Marcotuli, I., Gadaleta, A., Mangini, G., Signorile, A.M., Zacheo, S.A., Blanco, A., Simeone, R., Colasuonno, P. 2017. Development of a high-density SNP-based linkage map and detection of QTL for β-glucans, protein content, grain yield per spike and heading time in durum wheat. International Journal of Molecular Sciences 18:1329.
Miedaner, T., Hübner, M., Korzun, V., Schmiedchen, B., Bauer, E., Haseneyer, G., Wilde, P., Reif, J.C. 2012. Genetic architecture of complex agronomic traits examined in two testcross populations of rye (Secale cereale L.). BMC Genomics 13:706–719.
Mora, F., Quitral, Y.A., Matus, I., Russell, J., Waugh, R., del Pozo, A. 2016. SNP-Based QTL mapping of 15 complex traits in barley under rain-fed and well-watered conditions by a mixed modeling approach. Front. Plant Sci. 7:1–11.
Niedziela, A., Bednarek, P.T., Cichy, H., Budzianowski, G., Kilian, A., Anioł, A. 2012. Aluminum tolerance association mapping in triticale. BMC genomics 13:67.
Peng, B., Li, Y., Wang, Y., Liu, C., Liu, Z., Tan, W., Zhang, Y., Wang, D., Shi, Y., Sun, B., Song, Y., Wang, T., Li, Y. 2011. QTL analysis for yield components and kernel-related traits in maize across multi-environments. Theor. Appl. Genet. 122:1305–1320.
Ramya, P., Chaubal, A., Kulkarni, K., Gupta, L., Kadoo, N., Dhaliwal, H.S., Chhuneja, P., Lagu, M., Gupta, V. 2010. QTL mapping of 1000-kernel weight, kernel length, and kernel width in bread wheat (Triticum aestivum L.). J. Appl. Genet. 51:421–429.
Strang, E.J.P., Eklund, M., Rosenfelder, P., Sauer, N., Htoo, J.K., Mosenthin, R. 2016. Standardized ileal digestibility and basal ileal endogenous loss of amino acids associated with triticale genotypes in growing pigs. J. Animal Sci. 94:316–319.
Tyrka, M., Chełkowski, J. 2004. Enhancing the resistance of triticale by using genes from wheat and rye. J. Appl. Genet. 45:283–296.
Tyrka, M., Bednarek, P.T., Kilian, A., Wędzony, M., Hura, T., Bauer, E. 2011. Genetic map of triticale compiling DArT, SSR and AFLP markers. Genome 54:391–401.
Tyrka, M., Tyrka, D., Wędzony, M. 2015. Genetic map of triticale integrating microsatellite, DArT and SNP markers. PLoS ONE 10:1–17.
Tyrka, M., Oleszczuk, S., Rabiza-Swider, J., Wos, H., Wędzony, M., Zimny, J., Ponitka, A., Ślusarkiewicz-Jarzina, A., Metzger, R.J., Baenziger, P.S., Lukaszewski, A.J. 2018. Populations of doubled haploids for genetic mapping in hexaploid winter triticale. Molecular Breeding 38(4):46.
Wang, R.X., Hai, L., Zhang, X.Y., You, G.X., Yan, C.S., Xiao, S.H. 2009. QTL mapping for grain filling rate and yield-related traits in RILs of the Chinese winter wheat population Heshangmai × Yu8679. Theor. Appl. Genet. 118:313–325.
Wang, S., Basten, C.J., Zeng, Z.B. 2007. Windows QTL Cartographer 2.5. Department of Statistics, North Carolina State University, Raleigh, NC. pp. 85.
Wang, J., Liu, W., Wang, H., Li, L., Wu, J., Yang, X., Li, X., Gao, A. 2011. QTL mapping of yield-related traits in the wheat germplasm 3228. Euphytica 177:277–292.
Würschum, T., Liu, W., Busemeyer, L., Tucker, M.R., Reif, J.C., Weissmann, E.A., Hahn, V., Ruckelshausen, A., Maurer, H.P. 2014. Mapping dynamic QTL for plant height in triticale. BMC Genetics 15:59.
Zhang, J., Gizaw, S.A., Bossolini, E., Hegarty, J., Howell, T., Carter, A.H., Akhunov, E., Dubcovsky, J. 2018. Identification and validation of QTL for grain yield and plant water status under contrasting water treatments in fall-sown spring wheats. Theoretical and Applied Genetics 131(8):1174–1759.
Zhou, Y., Conway, B., Miller, D., Marshall, D., Cooper, A., Murphy, P., Chao, S., Brown-Guedira, G., Costa, J. 2017. Quantitative trait loci mapping for spike characteristics in hexaploid wheat. The Plant Genome, 10:1–15.
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Wajdzik, K., Gołębiowska, G., Dyda, M. et al. The QTL Mapping of the Important Breeding Traits in Winter Triticale (×Triticosecale Wittm.). CEREAL RESEARCH COMMUNICATIONS 47, 395–408 (2019). https://doi.org/10.1556/0806.47.2019.24
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DOI: https://doi.org/10.1556/0806.47.2019.24