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QTL analysis for grain weight in common wheat

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Abstract

Quantitative trait loci (QTL) analysis for grain weight (GW = 1000 grain weight) in common wheat was conducted using a set of 100 recombinant inbred lines (RILs) derived from a cross ‘Rye Selection 111 (high GW) × Chinese Spring (low GW)’. The RILs and their two parental genotypes were evaluated for GW in six different environments (three locations × two years). Genotyping of RILs was carried out using 449 (30 SSRs, 299 AFLP and 120 SAMPL) polymorphic markers. Using the genotyping data of RILs, framework linkage maps were prepared for three chromosomes (1A, 2B, 7A), which were earlier identified by us to carry important/major genes for GW following monosomic analysis. QTL analysis for GW was conducted following genome-wide single marker regression analysis (SMA) and composite interval mapping (CIM) using molecular maps for the three chromosomes. Following SMA, 12 markers showed associations with GW, individual markers explaining 6.57% to 10.76% PV (phenotypic variation) for GW in individual environments. The high grain weight parent, Rye Selection111, which is an agronomically superior genotype, contributed favourable alleles for GW at six of the 12 marker loci identified through SMA. The CIM identified two stable and definitive QTLs, one each on chromosome arms 2BS and 7AS, which were also identified through SMA, and a third suggestive QTL on 1AS. These QTLs explained 9.06% to 19.85% PV for GW in different environments. The QTL for GW on 7AS is co-located with a QTL for heading date suggesting the occurrence of a QTL having a positive pleiotropic effect on the two traits. Some of the markers identified during the present study may prove useful for marker-assisted selection, while breeding for high GW in common wheat.

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References

  • Ammiraju JSS, Dholakia BB, Santra DK, Singh H, Lagu MD, Tamhankar SA, Dhaliwal HS, Rao VS, Gupta VS, Ranjekar PK (2001) Identification of inter simple sequence repeat (ISSR) markers associated with seed size in wheat. Theor Appl Genet 102:726–732

    Article  CAS  Google Scholar 

  • Araki E, Miura H, Sawada S (1999) Identification of genetic loci affecting amylose content and agronomic traits on chromosome 4A of wheat. Theor Appl Genet 98:977–984

    Article  CAS  Google Scholar 

  • Basten CJ, Weir BS, Zeng Z-B (1994) Zmap-a QTL Cartographer. In: Smith C, Gavora JS, Benkel J, Chesnais B, Fairfull W, Gibson JP, Kennedy BW, Burnsid EB (eds), Proceedings 5th world congress on genetics applied to livestock production: computing strategies and software. Guelph, Ontario Canada Vol. 22, pp. 65–66

  • Börner A, Schumann E, Fürste A, Cöster H, Leithold B, Röder MS, Weber WE (2002) Mapping of quantitative trait loci determining agronomic important characters in hexaploid wheat (Triticum aestivum L.). Theor Appl Genet 105:921–936

    Google Scholar 

  • Breseghello F, Sorrells ME (2005) Association mapping of kernel size and milling quality in wheat (Triticum aestivum L.) cultivars. Genetics 172: 1165--1177

    Google Scholar 

  • Campbell BT, Baenziger PS, Gill KS, Eskridge KM, Budak H, Erayman M, Dweikat I, Yen Y (2003) Identification of QTLs and environmental interactions associated with agronomic traits on chromosome 3A wheat. Crop Sci 43:1493–1505

    Article  CAS  Google Scholar 

  • Campbell KG, Bergman CJ, Gualberto DG, Anderson JA, Giroux MJ, Hareland G, Fulcher RG, Sorrells ME, Finney PL (1999) Quantitative trait loci associated with kernel traits in soft × hard wheat cross. Crop Sci 39:1184–1195

    Article  CAS  Google Scholar 

  • Churchill GA, Doerge RW (1994) Empirical threshold values for quantitative trait mapping. Genetics 138:963–971

    PubMed  CAS  Google Scholar 

  • Dholakia BB, Ammiraju JSS, Singh H, Lagu MD, Röder MS, Rao VS, Dhaliwal HS, Ranjekar PK, Gupta VS (2003) Molecular marker analysis of kernel size and shape in bread wheat. Plant Breed 122:392–395

    Article  CAS  Google Scholar 

  • Doerge RW, Churchill GA (1996) Permutation tests for multiple loci affecting a quantitative character. Genetics 142:285–294.

    Google Scholar 

  • Gandhi SM, Sanghi AK, Nathawat KS, Bhatnagar MP (1963) Genotypic variability and correlation coefficient relating to grain yield and a few other quantitative characters in Indian wheats. Indian J Genet 24:1–8

    Google Scholar 

  • Groos C, Robert N, Bervas E, Charmet G (2003) Genetic analysis of grain protein content, grain yield and thousand-kernel weight in bread wheat. Theor Appl Genet 106:1032–1040

    PubMed  CAS  Google Scholar 

  • Goud JV, Sridevi O (1988) Cytogenetic investigations of some quantitative characters in hexaploid wheat (Triticum aestivum L.) using F2 monosomic analysis. In: Miller TE, Koebner RMD (eds), Proceedings of the 7th international wheat genetics symposium. Institute of Plant Sci. Res., Cambridge Lab, Trumpington, Cambridge, UK, Vol. 1, pp 521–525

  • Huang XQ, Cöster H, Ganal MW, Röder MS (2003) Advanced backcross QTL analysis for the identification of quantitative trait loci alleles from wild relatives of wheat (Triticum aestivum L.). Theor Appl Genet 106:1379–1389

    Google Scholar 

  • Huang XQ, Kempf H, Ganal MW, Röder MS (2004) Advanced backcross QTL analysis in progenies derived from a cross between a German elite winter wheat variety and synthetic wheat (Triticum aestivum L.). Theor Appl Genet 109:933–943

    Google Scholar 

  • Kato K, Miura, H, Sawada S (2000) Mapping QTLs controlling grain yield and its components on chromosome 5A of wheat. Theor Appl Genet 101:1114–1121.

    Google Scholar 

  • Ketata H, Edwards LH, Smith EL (1976) Inheritance of eight agronomic characters in a winter wheat cross. Crop Sci 16:19–22.

    Article  Google Scholar 

  • Kosambi DD, (1944) The estimation of map distances from recombination values. Ann Eugen 12:172–175

    Google Scholar 

  • Lander ES, Green P, Abrahamson J, Barlow A, Daly MJ, Lincoln SE, Newburg L (1987) MAPMAKER: an interactive computer package for constructing primary genetic linkage maps of experimental and natural populations. Genomics 1:174–181

    Article  PubMed  CAS  Google Scholar 

  • Marza F, Bai G-H, Carver BF, Zhou W-C (2005) Quantitative trait loci for yield and related traits in the wheat population Ning7840 × Clark. Theor Appl Genet DOI 10.1007/s00122-005-0172-3

  • McNeal FH, Qualset CO, Baldridge DE, Stewart VR (1978) Selection of yield and yield components in wheat. Crop Sci 18:795–799.

    Article  Google Scholar 

  • Narasimhamoorthy B, Gill BS, Fritz AK, Nelson JC, Brown-Guedira GL (2006) Advanced backcross QTL analysis of a hard winter wheat × synthetic wheat population. Theor Appl Genet DOI 10.1007/s00122-005-0159-0

  • Prasad M, Varshney RK, Kumar A, Balyan HS, Sharma PC, Edwards KJ, Singh H, Dhaliwal HS, Roy JK, Gupta PK (1999) A microsatellite marker associated with a QTL for grain protein content on chromosomal arm 2DL of bread wheat. Theor Appl Genet 99:341–345

    Article  Google Scholar 

  • Quarrie SA, Steed A, Calestani C, Semikhodskii A, Lebreton C, Chinoy C, Steele N, Pljevljakusic D, Waterman E, Weyen J, Schondelmaier J, Habash DZ, Farmer P, Saker L, Clarkson DT, Abugalieva A, Yessimbekova M, Turuspekov Y, Abugalieva S, Tuberosa R, Sanguineti M-C, Hollington PA, Royo R, Aragues A, Dodig, D (2005) A high-density genetic map of hexaploid wheat (Triticum aestivum L.) from the cross Chinese Spring × SQ1 and its use to compare QTLs for grain yield across a range of environments. Theor Appl Genet 110:865–880

    Google Scholar 

  • Rasyad A, Van Sanfold DA (1992) Genetic and maternal variances and covariances of kernel growth traits in winter wheat. Crop Sci 32:1139–1143

    Article  Google Scholar 

  • Röder MS, Korzun V, Plaschke K, Wendehake J, Tixier MH, Leroy P, Ganal MW (1998) A microsatellite map of wheat. Genetics 149:2007–2023

    PubMed  Google Scholar 

  • Saghai-Maroof MA, Soliman KM, Jorgensen RA, Allard RW (1984) Ribosomal DNA spacer length polymorphisms in barley: Mendelian inheritance, chromosomal location, and population dynamics. Proc Natl Acad Sci USA 81:8014–8018

    Article  PubMed  CAS  Google Scholar 

  • SAS Institute (1996) The SAS system for Windows. Release 6.12. SAS Inst. Cary, North Carolina

  • Sasaki M, Moriyasu M, Morris, R, Schmidt JW (1968) Chromosomal location of genes for some quantitative characters of wheat using chromosomal substitution lines. In: Finley KW, Shepherd KW (eds), Proceedings of the 3rd international Wheat Genetics Symposium. Aust Acad Sci, Canberra, Australia, pp. 343–349

  • Shah MM, Gill KS, Baenziger PS, Yen Y, Kaeppler SM, Ariyarathne HM (1999) Molecular mapping of loci for agronomic traits on chromosome 3A of bread wheat. Crop Sci 39:1728–1732

    Article  CAS  Google Scholar 

  • Sidwell RJ, Smith EL, McNew RW (1976) Inheritance and interrelationships of grain yield and selected yield-related traits in a hard red winter wheat cross. Crop Sci 16:650–654

    Article  Google Scholar 

  • Somers D, Isaac P, Edwards K (2004) A high-density microsatellite consensus map for bread wheat (Triticum aestivum L.). Theor Appl Genet 109:1105–1114

    Google Scholar 

  • Varshney RK, Prasad M, Roy JK, Kumar N, Singh H, Dhaliwal HS, Balyan HS, Gupta PK (2000) Identification of eight chromosomes and a microsatellite marker on 1AS associated with QTLs for grain weight in bread wheat. Theor Appl Genet 100:1290–1294

    Article  CAS  Google Scholar 

  • Vos P, Hogers R, Bleeker M, Reijans M, van de Lee T, Hornes M, Frijters A, Pot J, Peleman J, Kuiper M, Zabeau M (1995) AFLP: A new technique for DNA fingerprinting. Nucleic Acids Res 23:4407–4414

    PubMed  CAS  Google Scholar 

  • Wang S, Basten CJ, Zeng Z, (2004) Window QTL cartographer. V2.0 Program in Statistical Genetics, North Carolina State University, North Carolina. http://www.statgen.ncsu.edu/qtlcart/WQTLCart.htm.

  • Witsenboer H, Vogel J, Michelmore RW (1997) Identification, genetic localization, and allelic diversity of selectively amplified microsatellite polymorphic loci in lettuce and wild relatives (Lactuca spp.). Genome 40:923–936

    Google Scholar 

Download references

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Authors and Affiliations

  1. Molecular Biology Laboratory, Department of Genetics & Plant Breeding, Ch. Charan Singh University, Meerut, 250 004, UP, India

    Neeraj Kumar, Harindra S. Balyan & Pushpendra K. Gupta

  2. Department of Agricultural Botany, Dr. Panjabrao Deshmukh Agricultural University, Akola, 444 104, MS, India

    Pawan L. Kulwal

  3. Department of Plant Molecular Biology, University of Delhi, South Campus, New Delhi, 110021, India

    Anupama Gaur, Akhilesh K. Tyagi, Jitendra P. Khurana & Paramjit Khurana

Authors
  1. Neeraj Kumar
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  2. Pawan L. Kulwal
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  3. Anupama Gaur
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  4. Akhilesh K. Tyagi
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  5. Jitendra P. Khurana
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  6. Paramjit Khurana
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  7. Harindra S. Balyan
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  8. Pushpendra K. Gupta
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Correspondence to Pushpendra K. Gupta.

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Kumar, N., Kulwal, P.L., Gaur, A. et al. QTL analysis for grain weight in common wheat. Euphytica 151, 135–144 (2006). https://doi.org/10.1007/s10681-006-9133-4

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  • DOI: https://doi.org/10.1007/s10681-006-9133-4

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