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Comparison of optimal conditions for mass production of carboxymethylcellulase by Escherichia coli JM109/A-68 with other recombinants in pilot-scale bioreactor

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Abstract

The optimal conditions for mass production of carboxymethylcellulase (CMCase) by E. coli JM109/A-68 were investigated and compared with other E. coli JM109 recombinants producing CMCase. The optimal agitation speed and aeration rate for cell growth of E. coli JM109/A- 68 were 500 rpm and 0.50 vvm in a 7 L bioreactor, whereas those for production of CMCase were 416 rpm and 0.95 vvm. The optimal vessel pressures for cell growth as well as production of CMCase in a 100 L bioreactor were 0.04 MPa. The maximal production of CMCase by E. coli JM109/A-68 under the optimized conditions in a 100 L bioreactor was 11.0 times higher than its wild type, B. velezensis A-68. Optimal conditions for mass production of CMCase by recombinants were different from those for wild strains. The higher production of CMCase by E. coli JM109/A-68 and other recombinant of E. coli seemed to result from its higher cell growth under the optimal conditions for dissolved oxygen and its mixed-growth associated production pattern compared to the growthassociated production of B. velezensis A-68.

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Authors and Affiliations

  1. Department of Medical Bioscience, Graduate School of Dong-A University, Busan, 49315, Korea

    Myung-Hwan Kim

  2. Hubei Provincial Cooperative Innovation Center of Industrial Fermentation, College of Bioengineering, Hubei University of Technology, Wuhan, 430-068, China

    Wa Gao

  3. ReSEAT Programs, Korea Institute of Science and Technology Information, Seoul, 02792, Korea

    Chung-Han Chung

  4. Department of Biotechnology, College of Natural Resources and Life Science, Dong-A University, Busan, 49315, Korea

    Jin-Woo Lee

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  1. Myung-Hwan Kim
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  2. Wa Gao
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  4. Jin-Woo Lee
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Correspondence to Jin-Woo Lee.

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Kim, MH., Gao, W., Chung, CH. et al. Comparison of optimal conditions for mass production of carboxymethylcellulase by Escherichia coli JM109/A-68 with other recombinants in pilot-scale bioreactor. Biotechnol Bioproc E 22, 142–149 (2017). https://doi.org/10.1007/s12257-017-0035-1

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  • DOI: https://doi.org/10.1007/s12257-017-0035-1

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