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Enhanced production of cellobiase by marine bacterium Cellulophaga lytica LBH-14 from rice bran under optimized conditions involved in dissolved oxygen

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Abstract

The optimal conditions for production of cellobiase by C. lytica LBH-14 at flask scale had been previously reported. In this study, parameters involved in dissolved oxygen in 7 and 100 L bioreactors were optimized for pilot-scale production of cellobiase. The optimal agitation speed and aeration rate for cell growth of C. lytica LBH-14 were 400 rpm and 1.11 vvm in a 7 L bioreactor, whereas those for production of cellobiase were 330 rpm and 0.70 vvm. The analysis of variance (ANOVA) implied that significant factor for cell growth was the aeration rate, whereas those for production of cellobiase were the aeration rate as well as the agitation speed. The optimal inner pressures for cell growth and production of cellobiase by C. lytica LBH-14 in a 100 L bioreactor were 0.00 and 0.06 MPa, respectively. The maximal production of cellobiase in a 100 L bioreactor under optimized conditions using rice bran was 140.1 U/mL, which was 1.52 times higher than that in a flask scale.

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References

  1. Han, M., S. Moon, Y. Kim, Y. Kim, B. Chung, and G. Choi (2014) Bioethanol production from ammonia percolated wheat straw. Biotechnol. Bioproc. Eng. 14: 606–611.

    Article  Google Scholar 

  2. Gao, W., S. U. Lee, J. H. Li, and J. W. Lee (2013) Enhanced production of carboxymethylcellulase by Cellulophaga lytica LBH-14 in pilot-scale bioreactor under optimized conditions involved in dissolved oxygen. Kor. J. Chem. Eng. 30: 1105–1110.

    Article  Google Scholar 

  3. Howard, R. L., E. Abotsi, E. L. Jansen von Rensburg, and S. Howard (2003) Lignocellulose biotechnology: Issues of bioconversion and enzyme production. Afr. J. Biotechnol. 2: 602–619.

    Article  CAS  Google Scholar 

  4. Wei, G. Y., Y. J. Lee, Y. J. Kim, I. H. Jin, J. H. Lee, C. H. Chung, and J. W. Lee (2010) Kinetic study on the pretreatment and enzymatic saccharification on rice hull for the production of fermentable sugars. Appl. Biochem. Biotechnol. 162: 1471–1482.

    Article  CAS  Google Scholar 

  5. Rajulan, R., K. S. Dhar, M. Nampoothiri, and A. Pandey (2011) Production of leucine amino peptidase in lab scale bioreactor using Streptomyces gendanensis. Bioresour. Technol. 102: 8171–8178.

    Article  Google Scholar 

  6. Jung, D. Y., C. W. Son, S. K. Kim, W. Gao, and J. W. Lee (2013) Enhanced production of heteropolysaccharide-7 by Beijerinckia indica HS-2001 in pilot-scaled bioreactor under optimized conditions involved in dissolved oxygen using sucrose-based medium. Biotechnol. Bioproc. Eng. 18: 94–103.

    Article  CAS  Google Scholar 

  7. Gao, W., E. J. Lee, S. U. Lee, J. H. Li, C. H. Chung, and J. W. Lee (2012) Enhanced carboxymethylcellulase production by a newly isolated marine bacterium Cellulophaga lytica LBH-14 from rice bran using response surface method. J. Microbiol. Biotechnol. 22: 1415–1425.

    Google Scholar 

  8. Cao, W., H. W. Kim, J. H. Li, and J. W. Lee (2013) Enhanced production of cellobiase by a marine bacterium, Cellulophga lytica LBH-14, in pilot-scaled bioreactor using rice bran. J. Life Sci. 23: 542–553.

    Article  Google Scholar 

  9. Kim, H. J, Y. J. Lee, W. Gao, C. H. Chung, C. W. Son, and J. W. Lee (2011) Statistical optimization of fermentation conditions and comparison of their influences on production of cellulases by Psychrophilic marine bacterium, Psychrobacter aquimaris LBH-10 using orthogonal array method. Biotechnol. Bioproc. Eng. 16: 542–548.

    Article  CAS  Google Scholar 

  10. Kim, H. J., Y. J. Lee, W. Gao, C. H. Chung, and J. W. Lee (2012) Optimization of salts in medium for production of carboxymethylcellulase by a psychrophilic marine bacterium, Psychrobacter aquimaris LBH-10 using two statistical method. Kor. J. Chem. Eng. 28: 384–391.

    Article  Google Scholar 

  11. Kim, Y. J., W. Gao, E. J. Lee, S. U. Lee, C. H. Chung, and J. W. Lee (2012) Enhanced production of carboxymethylcellulase by a newly isolated marine bacterium Bacillus atrophaeus LBH-18 using rice bran, a byproduct from the rice processing industry. J. Life Sci. 22: 1295–1306.

    Article  Google Scholar 

  12. Jo, K. I., Y. J. Lee, B. K. Kim, B. H. Lee, C. H. Jung, S. W. Nam, S. K. Kim, and J. W. Lee (2008) Pilot-scale production of carboxymethylcellulase from rice hull by Bacillus amyloliquefaciens DL-3. Biotechnol. Bioproc. Eng. 13: 182–188.

    Article  CAS  Google Scholar 

  13. Kim, H. J., W. Gao, Y. J. Lee, C. H. Chung, and J. W. Lee (2010) Characterization of acidic carboxymethylcellulase produced by a marine microorganism, Psychrobacter aquimaris LBH-10. J. Life Sci. 20: 487–495.

    Article  Google Scholar 

  14. Kim, B. K., B. H. Lee, Y. J. Lee, I. H. Jin, C. H. Chung, and J. W. Lee (2009) Purification and characterization of carboxymethyl-cellulase isolated from a marine bacterium, Bacillus subtilis sub sp. subtilis A-53. Enz. Microb. Technol. 44: 411–416.

    Article  CAS  Google Scholar 

  15. Kim, B. K., H. J. Kim, and J. W. Lee (2013) Rapid statistical optimization of cultural conditions for mass production of carboxymethylcellulase by a newly isolated marine bacterium, Bacillus velezensis A-68 from rice hulls. J. Life Sci. 23: 757–769.

    Article  Google Scholar 

  16. Lee, Y. J., H. J. Kim, W. Gao, C. H. Chung, and J. W. Lee (2011) Comparison of statistical methods for optimization of salts in medium for production of carboxymethylcelluase of Bacilluls amyloliquefaciens DL-3 by a recombinant E. coli JM109/DL-3. J. Life Sci. 21: 1205–1213.

    Article  Google Scholar 

  17. Lee, B. H., B. K. Kim, Y. J. Lee, C. H. Chung, and J. W. Lee (2010) Industrial scale of optimization for the production of carboxymethylcellulase from rice bran by a marine bacterium, Bacillus subtilis subsp. subtilis A-53. Enz. Microbiol. Technol. 46: 38–42.

    Article  CAS  Google Scholar 

  18. Giavasis, I., L. M. Harvey, and B. McNeil (2006) The effect of agitation and aeration on the synthesis and molecular weight of gellan batch cultures of Sphingomonas paucimobilis. Enz. Microb. Technol. 38: 101–108.

    Article  CAS  Google Scholar 

  19. Elibol, M. and D. Ozer (2000) Influence of oxygen transfer on lipase production by Rhizopus arrhizus. Proc. Biochem. 36: 325–329.

    Article  Google Scholar 

  20. Feng, Y., Z. He, S. L. Ong, J. Hu, Z. Zhang, and W. J. Ng (2003) Optimization of agitation, aeration, and temperature conditions for maximum β-mannanase production. Enz. Microb. Technol. 32: 282–289.

    Article  CAS  Google Scholar 

  21. Lee, Y. J., H. J. Kim, W. Gao, C. H. Chung, and J. W. Lee (2012) Statistical optimization for production of carboxymethylcellulase of Bacillus amyloliquifaciences DL-3 by a recombinant Escherichia coli JM109/DL-3 from rice bran using response surface method. Biotechnol. Bioproc. Eng. 17: 227–235.

    Article  CAS  Google Scholar 

  22. Lee, E. J., B. H. Lee, B. K. Kim, and J. W. Lee (2013) Enhanced production of carboxymethylcellulase of a marine microorganism, Bacillus subtilis subsp. subtilis A-53 in a pilot-scaled bioreactor by a recombinant Escherichia coli JM109/A-53 from rice bran. Mol. Biol. Rep. 40: 3609–3621.

    Article  CAS  Google Scholar 

  23. Seo, H. P., C. H. Chung, S. K. Kim, R. A. Gross, D. L. Kaplan, and J. W. Lee (2004) Mass production of pullulan with optimized concentrations of carbon and nitrogen sources by Aureobaisdium pullulans HP-2001 in a 100 L bioreactor with the inner pressure. J. Microbial. Biotechnol. 14: 237–242.

    CAS  Google Scholar 

  24. Charoenrat, T., M. Ketudat-Cairns, M. Jahi, A. Veide, and S. Enfors (2006) Increased total air pressure versus oxygen limitation for enhanced oxygen transfer and product formation in a Pichia pastoris recombinant protein process. Biochem. Eng. J. 30: 205–211.

    Article  CAS  Google Scholar 

  25. Sukumaran, R. K., R. R. Singhania, G. M. Mathew, and A. Pandey (2009) Cellulase production using biomass feed stock and its application in lignocellulose saccharification for bio-ethanol production. Renew. Energy 34: 421–424.

    Article  CAS  Google Scholar 

  26. Kim, H. J., W. Gao, C. H. Chung, and J. W. Lee (2011) Statistical optimization for production of carboxymethylcellulase from rice hulls by a new isolated marine microorganism Bacillus licheniformis LBH-52 using response surface method. J. Life Sci. 21: 1083–1093.

    Article  Google Scholar 

  27. de Groot, M. J., P. J. van ee Vondervoort, R. P. de Vries, P. A. Vankuyk, G. J. Ruijter, and J. Visser (2003) Isolation and characterization of two specific regulatory Aspergillus niger mutants show antagonistic regulation of arabinan and xylan metabolism. Microbiol. 149: 1183–1191.

    Article  Google Scholar 

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Authors and Affiliations

  1. Department of Biotechnology, College of Natural Resources and Life Science, Dong-A University, Busan, 604-714, Korea

    Wa Gao & Jin-Woo Lee

  2. ReSEAT Programs, Korea Institute of Science and Technology Information, Seoul, 130-741, Korea

    Chung-Han Chung

  3. College of Plant Science & Technology, Huazhong Agricultural University, Wuhan, Hebei, 430-070, China

    Jianghong Li

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  1. Wa Gao
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  2. Chung-Han Chung
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  3. Jianghong Li
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  4. Jin-Woo Lee
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Correspondence to Jin-Woo Lee.

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Gao, W., Chung, CH., Li, J. et al. Enhanced production of cellobiase by marine bacterium Cellulophaga lytica LBH-14 from rice bran under optimized conditions involved in dissolved oxygen. Biotechnol Bioproc E 20, 131–138 (2015). https://doi.org/10.1007/s12257-014-0486-6

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  • DOI: https://doi.org/10.1007/s12257-014-0486-6

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