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Pretreatment and saccharification of rice hulls for the production of fermentable sugars

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Abstract

Hydrolytic conditions of rice hulls by acid and alkaline treatments before enzymatic saccharification were optimized in this study. Based on the results of single-factor experiments and an orthogonal array experiment, reaction time was found to be the most important factor for the acidic hydrolysis of rice hulls. Maximum yield of sugars from 1 g of rice hulls by acidic treatment under optimized conditions was 213.6 mg. The yield of lignin removal from acidic pretreated rice hulls by alkaline treatment increased with increase in reaction temperature and time. The amount of sugars obtained from 1 g of pretreated rice hulls by enzymatic saccharification was 307.7 mg, and the conversion rate of sugars from crude fibers in pretreated rice hulls was about 72%. Instrumental analyses with FTIR and SEM indicated that lignin in rice hulls was partially removed by alkaline treatment, and the structure of rice hulls became deformed and more fibers were exposed to cellulases after acidic treatment.

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Authors and Affiliations

  1. School of Life Science, Soochow University, Suzhou, 215-123, China

    Gong-Yuan Wei

  2. Department of Medical Bioscience, Dong-A University, Busan, 604-714, Korea

    Wa Gao

  3. Department of Biotechnology, Graduate School of Dong-A University, Busan, 604-714, Korea

    II-Hyuck Jin, Chung-Han Chung & Jin-Woo Lee

  4. BK21 Bio-Silver Project of Dong-A University, Busan, 604-714, Korea

    Gong-Yuan Wei, Wa Gao, Jai-Heon Lee, Chung-Han Chung & Jin-Woo Lee

  5. Research Facility Center of Dong-A University, Busan, 604-714, Korea

    Su-Young Yoo

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  1. Gong-Yuan Wei
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  2. Wa Gao
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  7. Jin-Woo Lee
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Correspondence to Jin-Woo Lee.

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Wei, GY., Gao, W., Jin, IH. et al. Pretreatment and saccharification of rice hulls for the production of fermentable sugars. Biotechnol Bioproc E 14, 828–834 (2009). https://doi.org/10.1007/s12257-009-0029-8

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  • DOI: https://doi.org/10.1007/s12257-009-0029-8

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