Skip to main content
SpringerLink
Log in

Pilot-scale production of carboxymethylcellulase from rice hull by Bacillus amyloliquefaciens DL-3

  • Published:
Biotechnology and Bioprocess Engineering Aims and scope Submit manuscript

Abstract

Optimal conditions for pilot-scale production of the carboxymethylcellulase (CMCase) by Bacillus amyloliquefaciens DL-3 were investigated. The best carbon and nitrogen sources for the production of CMCase by B. amyloliquefaciens DL-3 were found to be rice hull and peptone and their optimal concentrations were 5.0 and 0.20% (w/v), respectively. Optimal temperature and initial pH for the production of CMCase were 37°C and 6.8. Optimal agitation speed and aeration rate for the production of CMCase were 300 rpm and 1.0 vvm in a 7 L bioreactor, which were different from those for the cell growth of B. amyloliquefaciens DL-3. The highest productions of CMCase by B. amyloliquefaciens DL-3 from 5.0% (w/v) rice hull as a carbon source under optimal conditions in a 7 or 100 L bioreactor were 220 and 367 U/mL, respectively.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Haq, I., H. Ashraf, J. Iqbal, and M. A. Qadeer (2003) Production of alpha amylase by Bacillus licheniformis using an economical medium. Bioresour. Technol. 87: 57–61.

    Article  Google Scholar 

  2. Liming, X. and S. Xueliang (2004) High-yield cellulase production by Trichoderma reesei ZU-02 on corn cob residue. Bioresour. Technol. 91: 259–262.

    Article  CAS  Google Scholar 

  3. Olsson, L. and B. Hahn-Hagerdal (1996) Fermentation of lignocellulosic hydrolysates for ethanol production. Enzyme Microb. Technol. 18: 312–331.

    Article  CAS  Google Scholar 

  4. Luo, J., L. Xia, J. Lin, and P. Cen (1997) Kinetics of simultaneous saccharification and lactic acid fermentation processes. Biotechnol. Prog. 13: 762–767.

    Article  CAS  Google Scholar 

  5. Baek, S. C. and Y. J. Kwon (2007) Optimization of the pretreatment of rice straw hemicellulosic hydrolyzates for microbial production of xylitol. Biotechnol. Bioprocess Eng. 12: 404–409.

    CAS  Google Scholar 

  6. Min, B. J., Y. S. Park, S. W. Kang, Y. S. Song, J. H. Lee, C. H. Park, C. W. Kim, and S. W. Kim (2007) Statistical optimization of medium components for the production of xylanase by Aspergillus niger KK2 in submerged cultivation. Biotechnol. Bioprocess Eng. 12: 302–307.

    CAS  Google Scholar 

  7. Takagi, M., S. Abe, S. Suzuki, G. H. Emert, and N. Yata (1977) A method for production of ethanol directly from cellulose using cellulase and yeast. pp. 551–571. In: T. K. Ghose. Proceedings of Bioconversion Symposium. Delhi, India.

  8. Ballesteros, M., J. M. Oliva, M. J. Negro, P. Manzanares, and I. Ballesteros (2004) Ethanol from lignocellulosic materials by a simultaneous saccharification and fermentation process (SFS) with Kluyveromyces marxianus CECT 10875. Process Biochem. 39: 1843–1848.

    Article  CAS  Google Scholar 

  9. Saha, B. C. and M. A. Cotta (2007) Enzymatic saccharification and fermentation of alkaline peroxide pretreated rice hull to ethanol. Enzyme Microb. Technol. 41: 528–532.

    Article  CAS  Google Scholar 

  10. Saha, B. C., L. B. Iten, M. A. Cotta, and Y. V. Wu (2005) Dilute acid pretreatment, enzymatic saccharification, and fermentation of rice hulls to ethanol. Biotechnol. Prog. 21: 816–822.

    Article  CAS  Google Scholar 

  11. Kim. K. C., S. S. Yoo, Y. A. OH, and S. J. Kim (2003) Isolation and characteristics of Trichoderma harzianum FJ1 producing cellulases and xyanase. J. Microbiol. Biotechnol. 13: 1–8.

    Google Scholar 

  12. Cai, Y. J., S. J. Chapman, J. A. Buswell, and S. T. Chang (1999) Production and distribution of endoglucanase, cellobiohydrolase, and β-glucosidase components of the cellulolytic system of Volvariella volvacea, the edible straw mushroom. Appl. Environ. Microbiol. 65: 553–559.

    CAS  Google Scholar 

  13. Brich, P. R., P. F. Sims, and P. Broda (1995) Substrate-dependent differential splicing of introns in the regions encoding the cellulose binding domains of two exocellobiohydrolase I-like genes in Phanerochaete chryscsporium. Appl. Environ. Microbiol. 61: 3741–3744.

    Google Scholar 

  14. Chen, H., X. Li, and L. G. Ljungdahl (1994) Isolation and properties of an extracellular β-glucosidase from the polycentric rumen fungus Orpinomyces sp. strain PC-2. Appl. Environ. Microbiol. 60: 64–70.

    CAS  Google Scholar 

  15. Kim, K. C., S. W. Kim, M. J. Kim, and S. J. Kim (2005) Saccharification of foodwastes using cellulolytic and amylolytic enzymes from Trichoderma harzianum FJ1 and its kinetics. Biotechnol. Bioprocess Eng. 10: 52–59.

    CAS  Google Scholar 

  16. Krishna, C. (1999) Production of bacterial cellulases by solid state bioprocessing of banana wastes. Bioresour. Technol. 69: 231–239.

    Article  CAS  Google Scholar 

  17. Wood, T. M. (1992) Fungal cellulases. Biochem. Soc. Trans. 20: 46–53.

    CAS  Google Scholar 

  18. Suto, M. and F. Tomita (2001) Induction and catabolite repression mechanisms of cellulase in fungi. J. Biosci. Bioeng. 92: 305–311.

    Article  CAS  Google Scholar 

  19. Lee, Y. J., B. K. Kim, B. H. Lee, K. I. Jo, N. K. Lee, C. H. Chung, Y. C. Lee, and J. W. Lee (2008) Purification and characterization of cellulase produced by Bacillus amyloliquefaciens DL-3 utilizing rice hull. Bioresour. Technol. 99: 378–386.

    Article  CAS  Google Scholar 

  20. Jung, H. K., J. H. Hong, S. C. Park, B. K. Park, D. H. Nam, and S. D. Kim (2007) Production and physicochemical characterization of β-glucan produced by Paenibacillus polymyxa JB115. Biotechnol. Bioprocess Eng. 12: 713–719.

    CAS  Google Scholar 

  21. Lejeune, R. and G. V. Baron (1995) Effect of agitation on growth and enzyme production of Trichoderma reesei in batch fermentation. Appl. Microbiol. Biotechnol. 43: 249–258.

    Article  CAS  Google Scholar 

  22. Domingues, F. C., J. A. Queiroz, J. M. S. Cabral, and L. P. Fonseca (2000) The influence of culture conditions on mycelial structure and cellulase production by Trichoderma reesei Rut C-30. Enzyme Microb. Technol. 26: 394–401.

    Article  CAS  Google Scholar 

  23. Lee, S. M. and Y. M. Koo (2001) Pilot-scale production of cellulase using Trichoderma reesei Rut C-30 in fed-batch mode. J. Microbiol. Biotechnol. 11: 229–233.

    Article  CAS  Google Scholar 

  24. Okolo, J. C., S. K. C. Obi, and F. J. C. Odibo (1998) Purification and characterization of two distinct carboxymethylcellulases of Paecilomyces sp. Bioresour. Technol. 66: 231–234.

    Article  CAS  Google Scholar 

  25. Chen, P., T. Wei, Y. Chang, and L. Lin (2004) Purification and characterization of carboxymethyl cellulase from Sinorhizobium fredii. Bot. Bull. Acad. Sin. 45: 111–118.

    CAS  Google Scholar 

  26. Mayende, L., B. S. Wilhelmi, and B. I. Pletschke (2006) Cellulases (CMCases) and polyphenol oxidases from thermophilic Bacillus spp. isolated from compost. Soil Biol. Biochem. 38: 2963–2966.

    Article  CAS  Google Scholar 

  27. Ingram, L. O. and J. B. Doran (1995) Conversion of cellulosic materials to ethanol. FEMS Microbiol. Rev. 16: 235–241.

    Article  CAS  Google Scholar 

  28. Park, E. Y., Y. Ikeda, and N. Okuda (2002) Empirical evaluation of cellulase on enzymatic hydrolysis of waste office paper. Biotechnol. Bioprocess Eng. 7: 268–274.

    Article  CAS  Google Scholar 

  29. Mielenz, J. R. (2001) Ethanol production from biomass: technology and commercialization status. Curr. Opin. Microbiol. 4: 324–329.

    Article  CAS  Google Scholar 

  30. Yu, X. B., J. H. Nam, H. S. Yun, and Y. M. Koo (1998) Optimization of cellulase production in batch fermentation by Trichoderma reesei. Biotechnol. Bioprocess Eng. 3: 44–47.

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Biotechnology, College of Natural Resources and Life Science, Dong-A University, Busan, 604-714, Korea

    Kang-Ik Jo, You-Jung Lee, Bo-Kyung Kim, Bo-Hwa Lee, Chung-Han Chung & Jin-Woo Lee

  2. Department of Biotechnology and Bioengineering, College of Engineering, Dong-Eui University, Busan, 614-714, Korea

    Soo-Wan Nam

  3. Department of Biotechnology and Bioengineering, College of Fisheries Science, Pukyung National University, Busan, 608-737, Korea

    Sung-Koo Kim

Authors
  1. Kang-Ik Jo
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. You-Jung Lee
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Bo-Kyung Kim
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Bo-Hwa Lee
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Chung-Han Chung
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Soo-Wan Nam
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. Sung-Koo Kim
    View author publications

    You can also search for this author in PubMed Google Scholar

  8. Jin-Woo Lee
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Jin-Woo Lee.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Jo, KI., Lee, YJ., Kim, BK. et al. Pilot-scale production of carboxymethylcellulase from rice hull by Bacillus amyloliquefaciens DL-3. Biotechnol Bioproc E 13, 182–188 (2008). https://doi.org/10.1007/s12257-007-0149-y

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s12257-007-0149-y

Keywords

Search

Navigation