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Enhanced production of carboxymethylcellulase of Bacillus subtilis subsp. subtilis A-53 by a recombinant Escherichia coli JM109/A-53 with pH and temperature shifts

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Abstract

Effect of shifts in pH of the medium and temperature on cell growth and production of carboxymethylcellulase (CMCase) by a recombinant Escherichia coli JM109/A-53 was investigated. Lower initial pH (pH 7.0) of the medium and higher temperature (40 °C) stimulated cell growth of E. coli JM109/A-53, whereas higher initial pH (pH 8.0) and lower temperature (35 °C) enhanced production of CMCase. The maximal production of CMCase was obtained when initial pH of 7.0 and temperature of 40 °C were simultaneously changed to 8.0 and 35 °C after 36 h. The production of CMCase with simple shifts in pH and temperature was 783.1 U/mL, which was 2.13 times higher than that under optimal conditions for cell growth. This indicated that a simple process with shifts in pH and temperature could be economically applied for enhanced production of CMCase by E. coli JM109/A-53 with industrial scales.

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Authors and Affiliations

  1. Department of Medical Bioscience, Graduate School of Dong-A University, Busan, 604-714, Korea

    Eun-Jung Lee

  2. Department of Biotechnology, Dong-A University, Busan, 604-714, Korea

    Wa Gao & Jin-Woo Lee

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  1. Eun-Jung Lee
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  2. Wa Gao
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  3. Jin-Woo Lee
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Correspondence to Jin-Woo Lee.

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Lee, EJ., Gao, W. & Lee, JW. Enhanced production of carboxymethylcellulase of Bacillus subtilis subsp. subtilis A-53 by a recombinant Escherichia coli JM109/A-53 with pH and temperature shifts. Korean J. Chem. Eng. 32, 113–117 (2015). https://doi.org/10.1007/s11814-014-0160-x

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  • DOI: https://doi.org/10.1007/s11814-014-0160-x

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