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Statistical optimization of fermentation conditions and comparison of their influences on production of cellulases by a psychrophilic marine bacterium, Psychrobacter aquimaris LBH-10 using orthogonal array method

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Abstract

The optimal conditions for the production of cellulases by a marine bacterium, Psychrobacter aquimaris LBH-10, were established and their effects were compared using orthogonal array experiments based on the Taguchi method. The optimal conditions of rice bran, peptone and initial pH for the production of avicelase and CMCase by P. aquimaris LBH-10 were 50.0, 3.0, and 8.0 g/L, respectively, whereas those for filter paperase (FPase) were 100.0, 3.0, and 8.0 g/L, respectively. Rice bran was found to be the most important factor for the production of cellulases based on the calculated percentage of participation P (%) from an analysis of the variance (ANOVA). The optimal temperature for the cell growth of P. aquimaris LBH-10 was 25°C, whereas that for the production of avicelase, CMCase and FPase was 30°C. The optimal agitation speed and aeration rate for cell growth was 400 rpm and 1.5 vvm, respectively, whereas those for the production of CMCase were 300 rpm and 1.0 vvm, respectively. Aeration was found to be more important for cell growth and CMCase production than agitation. The maximum production of avicelase, CMCase and FPase in a 100 L bioreactor for 72 h under optimized conditions was 83.2, 388.7, and 75.4 U/mL, respectively.

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Authors and Affiliations

  1. Department of Medical Bioscience, Graduate School of Donga-A University, Busan, 604-714, Korea

    Hye-Jin Kim, You-Jung Lee & Wa Gao

  2. BK21 Bio-Silver Program of Dong-A University, Busan, 604-714, Korea

    Hye-Jin Kim, You-Jung Lee, Wa Gao, Chung-Han Chung & Jin-Woo Lee

  3. Department of Biotechnology, College of Natural Resources and Life Science, Dong-A University, Busan, 604-714, Korea

    Chung-Han Chung & Jin-Woo Lee

  4. Korea Biosolution Co., Busan, 612-020, Korea

    Chang-Woo Son

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  1. Hye-Jin Kim
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Correspondence to Jin-Woo Lee.

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Kim, HJ., Lee, YJ., Gao, W. et al. Statistical optimization of fermentation conditions and comparison of their influences on production of cellulases by a psychrophilic marine bacterium, Psychrobacter aquimaris LBH-10 using orthogonal array method. Biotechnol Bioproc E 16, 542–548 (2011). https://doi.org/10.1007/s12257-010-0457-5

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  • DOI: https://doi.org/10.1007/s12257-010-0457-5

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