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Expression of a Pennisetum glaucum gene DREB2A confers enhanced heat, drought and salinity tolerance in transgenic Arabidopsis

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Abstract

Background

Pearl millet (Pennisetum glaucum) is an essential cereal crop, whose growth and yield are not impacted by abiotic stresses, such as drought, heat, and cold. The DREB transcription factors (TF) are some of the largest groups of TFs in plants and play varied roles in plant stress response and signal transduction.

Methods and results

In the present study, PgDREB2A gene encoding a DREB transcription factor in pearl millet was functionally characterized in Arabidopsis. DREB2A proteins contain a conserved domain that binds toethylene responsive element binding factors. Three different T1 transgenic lines overexpressing PgDREB2A gene were identified by Southern blot. Quantitative real-time polymerase chain reaction exhibited that PgDREB2A could be induced under drought conditions. As compared with the control, PgDREB2A overexpressing transgenic Arabidopsis showed increased rate of seed germination and root growth in transgenic lines under higher concentrations of mannitol, NaCl, ABA, heat and cold stress. Additionally, PgDREB2A transgenic lines showed enhanced durability after rehydration and tolerance to drought and salt stress was augmented with increased proline and reduced MDA build-up and diminishing water loss.

Conclusions

Results from this study suggested that PgDREB2A as a transcription factor may improve endurance to various abiotic stresses and can be employed for developing crops tolerant to abiotic stresses.

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Acknowledgements

Authors are thankful to Director, ICAR-NIPB, New Delhi, India for providing necessary facilities and funds. Director IARI, New Delhi, India is duly thanked for National Phytotron Facilities. Award of fellowship to the first author by PG School-IARI and Department of Biotechnology (DBT), Government of India is duly acknowledged.

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  1. National Institute for Plant Biotechnology, Pusa Campus, New Delhi, India

    Rajendra Prasad Meena, Gourab Ghosh, Harinder Vishwakarma & Jasdeep Chatrath Padaria

  2. PG School, ICAR-Indian Agricultural Research Institute, Pusa Campus, New Delhi, 110012, India

    Rajendra Prasad Meena & Jasdeep Chatrath Padaria

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  1. Rajendra Prasad Meena
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JCP conceptualized the experiments, interpreted the results and made the final draft. RPM conducted the experiments and interpreted the results. GG and HV supported in experiments, interpreted the results, statistical analysis of data obtained and prepared the manuscript. All the authors have read and approved the final manuscript.

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Correspondence to Jasdeep Chatrath Padaria.

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Meena, R.P., Ghosh, G., Vishwakarma, H. et al. Expression of a Pennisetum glaucum gene DREB2A confers enhanced heat, drought and salinity tolerance in transgenic Arabidopsis. Mol Biol Rep 49, 7347–7358 (2022). https://doi.org/10.1007/s11033-022-07527-6

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