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Enzymatic synthesis and modification of structured phospholipids: recent advances in enzyme preparation and biocatalytic processes

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Abstract

Phospholipids (PLs) containing specific polar head groups and fatty acids, artificially synthesized from a complex mixture of natural PLs, have considerable industrial applications. The biocatalytic approaches to synthesizing structured PLs are of great interest because the enzymes used show high selectivity and performance under mild conditions, leading to the generation of products that cannot easily be obtained by chemical catalysis. Although the limited supply of phospholipases (e.g., phospholipase D) has thus far been an obstacle to the widespread use of enzymatic processing, recent advances in enzyme preparation have opened up various applications for PL modification. In this review, attempts to increase the productivity and utility of microbial phospholipases and lipases are presented. We also summarize recent developments in enzyme-catalyzed modification of PLs, focusing particularly on the relevant reactions, bioreactor design, and novel proof-of-concept experiments.

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Acknowledgments

This work was supported by grants-in-aid from the Cross-ministerial Strategic Innovation Promotion Program (SIP) from Japan Science and Technology Agency, Japan.

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The authors declare that they have no competing interests.

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Authors and Affiliations

  1. Research & Development Laboratory, Bio-energy Corporation, 2-9-7 Minaminanamatsu, Amagasaki, 660-0053, Japan

    Shinji Hama

  2. Department of Chemical Science and Engineering, Graduate School of Engineering, Kobe University, 1-1 Rokkodai, Nada, Kobe, 657-8501, Japan

    Chiaki Ogino & Akihiko Kondo

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  1. Shinji Hama
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  2. Chiaki Ogino
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  3. Akihiko Kondo
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Correspondence to Akihiko Kondo.

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Hama, S., Ogino, C. & Kondo, A. Enzymatic synthesis and modification of structured phospholipids: recent advances in enzyme preparation and biocatalytic processes. Appl Microbiol Biotechnol 99, 7879–7891 (2015). https://doi.org/10.1007/s00253-015-6845-1

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