Abstract
The structural gene for phospholipase D (PLD) of an actinomycete, Streptoverticillium cinnamoneum, together with its promoter region was introduced into Streptomyces lividans using a shuttle vector—pUC702—for Escherichia coli and S. lividans. The transformant was found to secrete a large amount of PLD (about 2.0×104 U/l, 42 mg/l) when cultured in a jar fermentor. Both an initial glucose concentration of 17.5 g/l and the feeding of carbon and nitrogen sources are effective for efficient secretion of PLD; under these culture conditions, the amount of PLD secreted reached a maximum level (about 5.5×104 U/l, 118 mg/l) after about 60 h. In contrast to the original producer, Stv. cinnamoneum, which secretes only a small amount of PLD (about 1.1×103 U/l, 2 mg/l) along with other extracellular proteins, this heterologous expression system is markedly more efficient in production of secretory PLD.
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Acknowledgements
This study was supported in part by a Grant-in-aid from the Ministry of Education, Culture, Sports, Science and Technology, Japan (No. 12750699) and the Sasagawa Memorial Foundation.
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Ogino, C., Kanemasu, M., Hayashi, Y. et al. Over-expression system for secretory phospholipase D by Streptomyces lividans . Appl Microbiol Biotechnol 64, 823–828 (2004). https://doi.org/10.1007/s00253-003-1552-8
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DOI: https://doi.org/10.1007/s00253-003-1552-8