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Incorporation of omega-3 polyunsaturated fatty acids into soybean lecithin: effect of amines and divalent cations on transesterification by lipases

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Abstract

The transesterification of soybean lecithin with methyl esters of EPA and DHA in an organic solvent (hexane) using various commercially available lipases was studied. Lipases produced by Candida antarctica, Pseudomonas fluorescens, Burkholderia cepacia, Mucor miehei, Thermomyces lanuginosus and Rhizomucor miehei were compared, in the absence or presence of histidine, arginine, urea, Ca2+, Mg2+, or a combination of urea and divalent cations (additives at 5 % of the total lipid mass). Transesterification using the R. miehei enzyme reached 11.32 and 12.30 % in the presence of Ca2+ or Mg2+ respectively, and 8.58 and 9.31 % when urea was also added. These were the greatest degrees of transesterification obtained. The results suggest the potential use of this immobilized lipase as a catalyst for interesterification reactions in organic solvent systems with low water content.

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Authors and Affiliations

  1. Department of Biology, Chemistry and Geography, Université du Québec à Rimouski, Rimouski, QC, Canada

    Nabil Marsaoui & Serge Laplante

  2. Laboratoires des Microorganismes et Biomolécules Actives, Faculté des Sciences de Tunis, El Manar, Tunis, Tunisia

    Nabil Marsaoui, Aly Raies & Karim Naghmouchi

  3. Lethbridge Research Center, Agriculture and Agri-Food Canada, Lethbridge, AB, Canada

    Karim Naghmouchi

  4. Biomass Valorisation Division, Quebec’s aquaculture and fisheries innovation centre (MERINOV), Gaspé, QC, Canada

    Serge Laplante

Authors
  1. Nabil Marsaoui
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  2. Serge Laplante
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  3. Aly Raies
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  4. Karim Naghmouchi
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Correspondence to Serge Laplante or Karim Naghmouchi.

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Marsaoui, N., Laplante, S., Raies, A. et al. Incorporation of omega-3 polyunsaturated fatty acids into soybean lecithin: effect of amines and divalent cations on transesterification by lipases. World J Microbiol Biotechnol 29, 2233–2238 (2013). https://doi.org/10.1007/s11274-013-1388-z

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  • DOI: https://doi.org/10.1007/s11274-013-1388-z

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