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Molecular cytogenetic characterization of alien introgressions with gene Fhb3 for resistance to Fusarium head blight disease of wheat

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Abstract

Fusarium head blight (FHB) resistance was identified in the alien species Leymus racemosus, and wheat-Leymus introgression lines with FHB resistance were reported previously. Detailed molecular cytogenetic analysis of alien introgressions T01, T09, and T14 and the mapping of Fhb3, a new gene for FHB resistance, are reported here. The introgression line T09 had an unknown wheat-Leymus translocation chromosome. A total of 36 RFLP markers selected from the seven homoeologous groups of wheat were used to characterize T09 and determine the homoeologous relationship of the introgressed Leymus chromosome with wheat. Only short arm markers for group 7 detected Leymus-specific fragments in T09, whereas 7AS-specific RFLP fragments were missing. C-banding and genomic in situ hybridization results indicated that T09 has a compensating Robertsonian translocation T7AL·7Lr#1S involving the long arm of wheat chromosome 7A and the short arm of Leymus chromosome 7Lr#1 substituting for chromosome arm 7AS of wheat. Introgression lines T01 (2n = 44) and T14 (2n = 44) each had two pairs of independent translocation chromosomes. T01 had T4BS·4BL-7Lr#1S + T4BL-7Lr#1S·5Lr#1S. T14 had T6BS·6BL-7Lr#1S + T6BL·5Lr#1S. These translocations were recovered in the progeny of the irradiated line Lr#1 (T5Lr#1S·7Lr#1S). The three translocation lines, T01, T09, and T14, and the disomic addition 7Lr#1 were consistently resistant to FHB in greenhouse point-inoculation experiments, whereas the disomic addition 5Lr#1 was susceptible. The data indicated that at least one novel FHB resistance gene from Leymus, designated Fhb3, resides in the distal region of the short arm of chromosome 7Lr#1, because the resistant translocation lines share a common distal segment of 7Lr#1S. Three PCR-based markers, BE586744-STS, BE404728-STS, and BE586111-STS, specific for 7Lr#1S were developed to expedite marker-assisted selection in breeding programs.

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References

  • Bai GH, Shaner G (2004) Management and resistance in wheat and barley to Fusarium head blight. Annu Rev Phytopathol 42:135–161

    Article  PubMed  CAS  Google Scholar 

  • Busch RH, McVey DV, Rauch T, Baumer J, Elsayed F (1984) Registration of ‘Wheaton’ wheat. Crop Sci 24:622

    Google Scholar 

  • Chen PD, Wang ZT, Wang SL, Huang L, Wang YZ, Liu DJ (1993) Transfer of scab resistance from Elymus gigantenus into common wheat. In: Li ZS, Xin ZY (eds) Proceedings of 8th international wheat genetic symposium, China Agricultural Scien-tech Press, Beijing 1:153–157

  • Chen PD, Wang ZT, Wang SL, Huang L, Wang YZ, Liu DJ (1995) Transfer of scab resistance from Elymus gigantenus into common wheat. III. Development of addition lines with wheat scab resistance (in Chinese with English abstract). Acta Genet Sin 22:206–216

    Google Scholar 

  • Chen PD, Sun WX, Liu WX, Yuan JH, Liu ZH, Wang SL, Liu DJ (1998) Development of wheat-Leymus racemosus translocation lines with scab resistance. In: Slinkard AE (ed) Proceedings of 9th international wheat genetic symposium, University Extension Press, Saskatoon, Canada 2:32–34

  • Chen PD, Liu WX, Yuan JH, Wang XE, Zhou B, Wang SL, Zhang SZ, Feng YG, Yang BJ, Liu GX, Liu DJ, Qi LL, Zhang P, Friebe B, Gill BS (2005) Development and characterization of wheat-Leymus racemosus translocation lines with resistance to Fusarium head blight. Theor Appl Genet 111:941–948

    Article  PubMed  Google Scholar 

  • Dewey DR (1984) The genome system of classification as a guide to intergeneric hybridization with perennial Triticeae. In: Gustafson JP (ed) Gene manipulation in plant improvement. Plenum Press, New York, pp 209–279

    Google Scholar 

  • Dill-Macky R (2003) Inoculation methods and evaluation of Fusarium head blight resistance in wheat. In: Leonard KJ, Bushnell WR (eds) Fusarium head blight of wheat and barley. Am Phytopathol Soc, St Paul, pp 184–210

    Google Scholar 

  • Endo TR (1988) Induction of chromosomal structural changes by a chromosome from Aegilops cylindrica L. in common wheat. J Hered 79:366–370

    Google Scholar 

  • Endo TR, Gill BS (1996) The deletion stocks of common wheat. J Hered 87:295–307

    CAS  Google Scholar 

  • Friebe B, Mukai Y, Dhaliwal HS, Martin TJ, Gill BS (1991) Identification of alien chromatin specifying resistance to wheat streak mosaic and greenbug in wheat germ plasm by C-banding and in situ hybridization. Theor Appl Genet 81:381–389

    Google Scholar 

  • Friebe B, Raupp Jiang J, WJ McIntosh RA, Gill BS (1996) Characterization of wheat-alien translocations conferring resistance to diseases and pests: current status. Euphytica 91:59–87

    Article  Google Scholar 

  • Friebe B, Zhang P, Linc G, Gill BS (2005) Robertsonian translocations in wheat arise by centric misdivision of univalents at anaphase I and rejoining of broken centromeres during interkinesis of meiosis II. Cytogenet Genome Res 109:293–297

    Article  PubMed  CAS  Google Scholar 

  • Gill BS, Friebe B, Endo TR (1991) Standard karyotype and nomenclature system for description of chromosome bands and structural aberrations in wheat (Triticum aestivum). Genome 34:830–839

    Google Scholar 

  • Hohmann U, Endo TR, Gill KS, Gill BS (1994) Comparison of genetic and physical maps of group 7 chromosomes from Triticum aestivum L. Mol Gen Genet 245:644–653

    Article  PubMed  CAS  Google Scholar 

  • Jiang J, Friebe B, Gill BS (1994) Recent advances in alien gene transfer in wheat. Euphytica 73:199–212

    Article  Google Scholar 

  • Jiang J, Gill BS (1998) Preferential male transmission of an alien chromosome in wheat. J Hered 89:87–89

    Article  Google Scholar 

  • Johnson DD, Flaskerud GK, Taylor RD, Satyanayana V (2003) Quantifying economic impacts of Fusarium head blight in wheat. In: Leonard KJ, Bushnell WR (eds) Fusarium head blight of wheat and barley. APS press, St Paul, pp 461–483

    Google Scholar 

  • Kishii M, Yamada T, Sasakuma T, Tsujimoto H (2004) Production of wheat-Leymus racemosus chromosome addition lines. Theor Appl Genet 109:255–260

    Article  PubMed  CAS  Google Scholar 

  • Kumar S, Gill BS, Faris JD (2007) Identification and characterization of segregation distortion loci along chromosome 5B in tetraploid wheat. Mol Genet Genomics 278:187–196

    Article  PubMed  CAS  Google Scholar 

  • Liu S, Anderson JA (2003) Marker assisted evaluation of Fusarium head blight resistant wheat germplasm. Crop Sci 43:760–766

    CAS  Google Scholar 

  • Ma ZQ, Röder M, Sorrells ME (1996) Frequencies and sequence characteristics of di-, tri-, and tetra-nucleotide microsatellites in wheat. Genome 39:123–130

    Article  PubMed  CAS  Google Scholar 

  • Marais GF (1990) Preferential transmission in bread wheat of a chromosome segment derived from Thinopyrum distichum (Thumb.) Löve. Plant Breed 104:152–159

    Article  Google Scholar 

  • Mujeeb-Kazi A, Rodriguez R (1981) An intergeneric hybrid of Triticum aestivum L X Elymus giganteus. J Hered 72:253–256

    Google Scholar 

  • Mullan DJ, Platteter A, Teakle NL, Appels R, Colmer TD, Anderson JM, Francki MG (2005) EST-derived SSR markers from defined regions of the wheat genome to identify Lophopyrum elongatum specific loci. Genome 48:811–822

    PubMed  CAS  Google Scholar 

  • Okamoto M (1957) Asynaptic effect of chromosome V. Wheat Inf Serv 5:6

    Google Scholar 

  • Qi LL, Chen PD, Liu DJ, Zhou B, Zhang SZ et al (1993) Development of translocation lines of Triticum aestivum with powdery mildew resistance introduced from Haynaldia villosa. In: Li ZS, Xin ZY (eds) Proceedings of 8th international wheat genetic symposium. Chinese Agricultural Scientech Press, Beijing, China, pp 333–337

  • Qi LL, Wang SL, Chen PD, Liu DJ, Friebe B, Gill BS (1997) Molecular cytogenetic analysis of Leymus recemosus chromosomes added to wheat. Theor Appl Genet 95:1084–1091

    Article  CAS  Google Scholar 

  • Qi LL, Gill BS (2001) High-density physical maps reveal that the dominant male-sterile gene Ms3 is located in a genomic region of low recombination in wheat and is not amenable to map-based cloning. Theor Appl Genet 103:998–1006

    Article  CAS  Google Scholar 

  • Qi LL, Echalier B, Friebe B, Gill BS (2003) Molecular characterization of a set of wheat deletion stocks for using in chromosome bin mapping of ESTs. Funct Integr Genomics 3:39–55

    PubMed  CAS  Google Scholar 

  • Qi LL, Echalier B, Chao S, Lazo GR, Butler GE, Anderson OD et al (2004) A chromosome bin map of 16, 000 expressed sequence tag loci and distribution of genes among the three genomes of polyploid wheat. Genetics 168:701–712

    Article  PubMed  CAS  Google Scholar 

  • Qi LL, Friebe B, Zhang P, Gill BS (2007) Homoeologous recombination, chromosome engineering and crop improvement. Chromosome Res 15:3–19

    Article  PubMed  CAS  Google Scholar 

  • Rajaram S, Mann CE, Ortis Ferrara G, Mujeeb-Kazi A (1983) Adaptation, stability and high yield potential of certain 1B/1R CIMMYT wheats. In: Sakamoto S (ed) Proceedings of 6th international wheat genetic symposium. Plant Germ-Plasm Institute, Kyoto, pp 613–621

  • Riley R, Chapman V (1958) Genetic control of cytologically diploid behavior of hexaploid wheat. Nature 182:713–715

    Article  Google Scholar 

  • Röder MS, Korzum V, Wendehake K, Plaschke J, Tixier M-H, Leroy P, Ganal M (1998) A microsatellite map of wheat. Genetics 149:2007–2023

    PubMed  Google Scholar 

  • Rozen S, Skaletsky HJ (2000) Primer3 on the WWW for general users and for biologist programmers. In: Krawetz S, Misener S (eds) Methods and protocols: methods in molecular biology. Bioinformatics Humana Press, Totowa, pp 365–386

    Google Scholar 

  • Sears ER (1952) Misdivision of univalents in common wheat. Chromosoma 4:535–550

    Article  PubMed  CAS  Google Scholar 

  • Sears ER (1954) The aneuploids of common wheat. Univ Mo Agric Exp Stn Bull 572:1–58

    Google Scholar 

  • Sears ER (1956) The transfer of leaf rust resistance from Aegilops umbellulata to wheat. Brookhaven Symp Biol 9:1–22

    Google Scholar 

  • Sears ER (1966) Nullisomic-tetrasomic combinations in hexaploid wheat. In: Riley R, Lewis KR (eds) Chromosome manipulations and plant genetics. Oliver and Boyd, Edinburgh, pp 29–45

    Google Scholar 

  • Sears ER (1977) An induced mutant with homoeologous pairing in common wheat. Can J Genet Cytol 19:585–593

    Google Scholar 

  • Sears ER, Okamoto M (1958) Intergenomic chromosome relationships in hexaploid wheat. In: Proc of 10th Int Congr Genet, Montreal, Canada 2:258–259

  • Sears ER, Steinitz-Sears LM (1978) The telocentric chromosomes of common wheat. In: Ramanujam S (ed) Proceedings of 5th international wheat genetics symposium. Ind Soc Gen Plt Breed. New Delhi, India, pp 389–407

  • Sharma D, Knott DR (1966) The transfer of leaf rust resistance from Agropyron to Triticum by irradiation. Can J Genet Cytol 8:137–143

    Google Scholar 

  • Sharma HC, Gill BS (1983) Current status of wide hybridization in wheat. Euphytica 32:17–31

    Article  Google Scholar 

  • Somers DJ, Isaac P, Edwards K (2004) A high-density microsatellite consensus map of bread wheat (Triticum aestivum L.). Theor Appl Genet 109:1105–1114

    Article  PubMed  CAS  Google Scholar 

  • Sourdille P, Singh S, Cadalen T, Brown-Guedira GL, Gay G, Qi LL, Gill BS, Dufour P, Murigneux A, Bernard M (2004) Microsatellite-based deletion bin system for the establishment of genetic-physical map relationships in wheat (Triticum aestivum L.). Funct Int Genomics 4:12–25

    Article  CAS  Google Scholar 

  • Wang SL, Qi LL, Chen PD, Liu DJ, Friebe B, Gill BS (1999) Molecular cytogenetic identification of wheat-Elymus tsukushiense introgression lines. Euphytica 107:217–224

    Article  Google Scholar 

  • Wang XE, Chen PD, Liu DJ, Zhang P, Zhou B, Friebe B, Gill BS (2001) Molecular cytogenetic characterization of Roegneria ciliaris chromosome additions in common wheat. Theor Appl Genet 102:651–657

    Article  CAS  Google Scholar 

  • Wang YN, Chen PD, Wang ZT, Liu DJ (1986) Transfer of useful germplasm from E. giganteus to common wheat. I. Production of wheat X E. giganteus hybrids. J Nanjing Agric Univ 1:10–14

    Google Scholar 

  • Wang YN, Chen PD, Wang ZT, Liu DJ (1991) Transfer of useful germplasm from E. giganteus to common wheat. II. Cytogenetics and scab resistance of backcross derivatives. J Nanjing Agric Univ 14:1–5

    CAS  Google Scholar 

  • Weng YQ, Liu DJ (1989) Morphology, scab resistance and cytogenetics of intergeneric hybrids of Triticum aestivum L. with Roegneria C. Koch (Agropyron species) (in Chinese with English abstract). Scentia Agric Sin 22:1–7

    Google Scholar 

  • Wood EA, Sebesta EE, Stark KJ (1974) Resistance of ‘Gaucho’ triticale to Schizaphis graminum. Environ Entomol 3:720–721

    Google Scholar 

  • Zhang P, Friebe B, Lukaszewski AJ, Gill BS (2001) The centromere structure in Robertsonian wheat-rye translocation chromosomes indicates that centric breakage-fusion can occur at different positions within the primary constriction. Chromosome 110:335–344

    Article  CAS  Google Scholar 

  • Zhang QP, Li Q, Wang XE, Wang HY, Lang SP et al (2005) Development and characterization of a Triticum aestivum-Haynaldia villosa translocation line T4VS·4DL conferring resistance to wheat spindle streak mosaic virus. Euphytica 145:317–320

    Article  CAS  Google Scholar 

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Acknowledgments

This project was supported by the McKnight Foundation, WGGRC, and the US Department of Agriculture under agreement no. 59-0790-7-075. This was a cooperative project with the US Wheat and Barley Scab Initiative (USBWI). Any opinions, findings, conclusions, or recommendations expressed in this publication are those of the authors and do not necessarily reflect the view of the US Department of Agriculture. We thank Dr. R. A. McIntosh and W. J. Raupp for critical reading of the manuscript and D. L. Wilson for excellent technical assistance. This is contribution number 08-339-J from the Kansas Agricultural Experiment Station, Kansas State University, Manhattan, KS 66506-5502, USA.

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Authors and Affiliations

  1. Wheat Genetic and Genomic Resources Center, Department of Plant Pathology, Kansas State University, Manhattan, KS, 66506-5502, USA

    L. L. Qi, Bernd Friebe & B. S. Gill

  2. ARS-USDA, Plant Science and Entomology Research Unit, Manhattan, KS, 66506-5502, USA

    M. O. Pumphrey

  3. The National Key Laboratory of Crop Genetics and Germplasm Enhancement, Nanjing Agricultural University, 210095, Nanjing, Jiangsu, People’s Republic of China

    P. D. Chen

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  1. L. L. Qi
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  2. M. O. Pumphrey
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  3. Bernd Friebe
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  4. P. D. Chen
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  5. B. S. Gill
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Correspondence to Bernd Friebe.

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Communicated by J. Dubcovsky.

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Qi, L.L., Pumphrey, M.O., Friebe, B. et al. Molecular cytogenetic characterization of alien introgressions with gene Fhb3 for resistance to Fusarium head blight disease of wheat. Theor Appl Genet 117, 1155–1166 (2008). https://doi.org/10.1007/s00122-008-0853-9

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