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Induction of 4VS chromosome recombinants using the CS ph1b mutant and mapping of the wheat yellow mosaic virus resistance gene from Haynaldia villosa

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Abstract

The wheat spindle streak mosaic virus (WSSMV) or wheat yellow mosaic virus (WYMV) resistance gene, Wss1, from Haynaldia villosa, was previously mapped to the chromosome arm 4VS by the development of 4V (4D) substitution and T4DL·4VS translocation lines. For better utilization and more accurate mapping of the Wss1, in this research, the CS ph1b mutant was used to induce new translocations with shortened 4VS chromosome fragments. Thirty-five homozygous translocations with different alien fragment sizes and breakpoints of 4VS were identified by GISH and molecular marker analysis. By field test, it was found that all the identified terminal translocations characterized as having smaller 4VS chromosome segments in the chromosome 4DS were highly resistant to WYMV, while all the interstitial translocations with 4VS inserted into the 4DS were WYMV susceptible. Marker analysis using 32 4VS-specific markers showed that both the terminal and interstitial translocations had different alien fragment sizes. Five specific markers could be detected in the WYMV-resistant terminal translocation line NAU421 with the shortest introduced 4VS fragment, indicating they can be used for marker-assisted selection in wheat breeding. Based on the resistance evaluation, GISH and molecular marker analysis of the available translocations, the gene(s) conferring the WYMV resistance on 4VS could be further cytologically mapped to the distal region of 4VS, immersed in the bin of FL 0.78–1.00. The newly developed small fragment translocations with WYMV resistance and 4VS specific markers have laid solid groundwork for the utilization in wheat breeding for WYMV resistance as well as further cloning of Wss1.

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Acknowledgments

This research was supported by National High Technology Research Program (‘863’ Program) of China (grant no. 2011AA100101, 2011AA10010201), the National Natural Science Foundation of China (grant no. 31201204), Natural Science Foundation of Jiangsu Province (grant no. BK2010448), Technology Support Program of Jiangsu Province (grant no. BE2012306), the Program of Introducing Talents of Discipline to Universities (No. B08025) and A Project Funded by the Priority Academic Program Development of Jiangsu Higher Education Institutions (PAPD).

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Authors and Affiliations

  1. State Key Laboratory of Crop Genetics and Germplasm Enhancement, Cytogenetics Institute, Nanjing Agricultural University, Nanjing, 210095, Jiangsu, China

    Renhui Zhao, Haiyan Wang, Jin Xiao, Qi Jia, Chunxia Yuan, Ruiqi Zhang, Aizhong Cao, Peidu Chen & Xiue Wang

  2. Department of Wheat Breeding, Agricultural Sciences Institute of the Lixiahe District, Yangzhou, 225007, Jiangsu, China

    Tongde Bie & Shunhe Cheng

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  1. Renhui Zhao
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  2. Haiyan Wang
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Correspondence to Xiue Wang.

Additional information

Communicated by I. D. Godwin.

R. Zhao and H. Wang contributed equally to this article.

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122_2013_2181_MOESM1_ESM.tif

Fig. S1 GISH and FISH patterns of chromosomes on RTC at mitotic metaphase of wheat–H. villosa terminal translocation lines. The probes were described in Fig. 5. (a-b) NAU422; (c-d) NAU 423; (e–f) NAU424; (g-h) NAU425; (i-j) NAU426; (k-l) NAU427; (m–n) NAU428; (o-p) NAU429; (q-r) NAU430; (s-r) NAU431. Scale bar = 10 μm (TIFF 3368 kb)

122_2013_2181_MOESM2_ESM.tif

Fig. S2 GISH and FISH patterns of chromosomes on RTC at mitotic metaphase of wheat–H. villosa interstitial translocation lines. The probes were described in Fig. 5. (a-b) NAU432; (c-d) NAU 433; (e–f) NAU434; (g-h) NAU435; (i-j) NAU436; (k-l) NAU437; (m–n) NAU438. Scale bar = 10 μm.(TIFF 2476 kb)

Table S1 Specific markers for chromosome 4V of H. villosa (DOC 110 kb)

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Zhao, R., Wang, H., Xiao, J. et al. Induction of 4VS chromosome recombinants using the CS ph1b mutant and mapping of the wheat yellow mosaic virus resistance gene from Haynaldia villosa . Theor Appl Genet 126, 2921–2930 (2013). https://doi.org/10.1007/s00122-013-2181-y

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