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DNA methylation changes detected by methylation-sensitive amplified polymorphism in the Pacific oyster (Crassostrea gigas) in response to salinity stress

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Abstract

Salinity is a considerable factor to the survival and distribution for a majority of marine organisms, the drawbacks of which are becoming a serious issue of aquaculture. DNA methylation, an extensively studied epigenetic modification in eukaryotes, plays a significant role in the regulation of gene expression in responding to environmental changes and triggering evolutionary consequences. The Pacific oyster Crassostrea gigas, as a eurythermal and euryhaline species, is considered to be tolerant to salinity fluctuation. In this study, fluorescent-labeled methylation-sensitive amplified polymorphism (F-MSAP) analysis was used to investigate the frequency and variation of DNA methylation in C. gigas under different salinity and time. The results showed that total methylation level was generally on a downward trend. At lower salinity, the total methylation level decreased at the earlier process and then increased during experiment process, but continued to shrink at the rest salinity. Fully methylation tended to better reflect the dynamics of total methylation. Recovery treatment showed that the extent and pattern of DNA methylation were difficult to return to the normal level in this research. The sequencing and BLAST analysis indicated that in salt stress most of the selected bands were closely related to the metabolism of nucleic acids and proteins, tropomyosin, and cellular transport, effecting on different biological processes of C. gigas. This work provides useful data to further elucidate the molecular mechanisms of salt stress response and tolerance in invertebrates.

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Acknowledgements

This study was supported by the grants from National Natural Science Foundation of China (31372524), Shandong Seed Project, Shandong Province (2016ZDJS06A06), and Qingdao National Laboratory for Marine Science and Technology (2015ASKJ02).

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Authors and Affiliations

  1. Key Laboratory of Mariculture, Ministry of Education, Ocean University of China, Qingdao, 266003, China

    Xin Zhang, Qi Li, Lingfeng Kong & Hong Yu

  2. Laboratory for Marine Fisheries Science and Food Production Processes, Qingdao National Laboratory for Marine Science and Technology, Qingdao, China

    Xin Zhang, Qi Li, Lingfeng Kong & Hong Yu

Authors
  1. Xin Zhang
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  2. Qi Li
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  3. Lingfeng Kong
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  4. Hong Yu
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Correspondence to Qi Li.

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Conflict of interest

Xin Zhang declares that she does not have conflict of interest. Qi Li declares that he does not have conflict of interest. Lingfeng Kong declares that he does not have conflict of interest. Hong Yu declares that she does not have conflict of interest.

Ethical statement

The research was conducted in the absence of any ethical issue on aquatic animal research.

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Zhang, X., Li, Q., Kong, L. et al. DNA methylation changes detected by methylation-sensitive amplified polymorphism in the Pacific oyster (Crassostrea gigas) in response to salinity stress. Genes Genom 39, 1173–1181 (2017). https://doi.org/10.1007/s13258-017-0583-y

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  • DOI: https://doi.org/10.1007/s13258-017-0583-y

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