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Analysis of DNA Methylation Level of Portunus trituberculatus Subjected to Low Salinity with Methylation-Sensitive Amplification Polymorphism

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Abstract

In this study, the methylation-sensitive amplification polymorphism (MSAP) was used to compare the genomic DNA methylation level of muscle, gill and hepatopancreas of Portunus trituberculatus subjected to salinity 12 for 30 days to illustrate the epigenetic mechanism of osmoregulation. Thirty primers were used to analyze the difference of methylation level of different tissues. The results showed that the baseline methylation level of muscle, hepatopancreas and gill was 47.31%, 22.94% and 17.69%, respectively. After exposed to low salinity stress, the methylation epiloci changed in the three tissues. Both demethylation and methylation processes occurred under low salinity stress. The methylation ratio decreased in muscle and gill but increased in hepatopancreas. These results indicated that DNA methylation is tissue-specific when P. trituberculatus responds to low salinity.

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References

  • Angers, B., Castonguay, E., and Massicotte, R., 2010. Environmentally induced phenotypes and DNA methylation: How to deal with unpredictable conditions until the next generation and after. Molecular Ecology, 19 (7): 7–1283.

    Article  Google Scholar 

  • Bird, A., 2002. DNA methylation patterns and epigenetic memory. Genes and Development, 16 (1): 1–6.

    Article  Google Scholar 

  • Cao, Z., Ding, W., Yu, J., Cao, L., and Wu, T., 2007. Differences in methylated loci among different grass carp individuals from one pair of parents. Acta Zoologica Sinica, 6: 1083–1088.

    Google Scholar 

  • Chen, G. Q., Jiang, M. M., Chen, B., Yang, Z. E., and Lin, C., 2006. Emergy analysis of Chinese agriculture. Agriculture Ecosystems and Environment, 115(1): 161–173.

    Article  Google Scholar 

  • Christensen, B. C., Houseman, E. A., Marsit, C. J., Zheng, S., Wrensch, M. R., Wiemels, J. L., Nelson, H. H., Karagas, M. R., Padbury, J. E., Bueno, R., Sugarbaker, D. J., Yeh, R. E., Wiencke, J. K., and Kelsey, K. E., 2009. Aging and environmental exposures alter tissue-specific DNA methylation dependent upon cpg island context. PLoS Genetics, 5 (8): e1000 602.

    Article  Google Scholar 

  • Csankovszki, G., Nagy, A., and Jaenisch, R., 2001. Synergism of Xist RNA, DNA methylation, and histone hypoacetylation in maintaining X chromosome inactivation. The Journal of Cell Biology, 153 (4): 4–773.

    Article  Google Scholar 

  • Doerfler, W., 1983. DNA methylation and gene activity. Cell, 52 (53): 93.

    Google Scholar 

  • Feng, X. D., Hong, L. Z., Hua, X. X., and Ping, X. I., 2005. Ehe influence of typhoon on the sea surface salinity in the warm pool of the western pacific. Acta Oceanologica Sinica, 27 (6): 6–343.

    Google Scholar 

  • Han, X. K., Gao, B. Q., Wang, H. F., Liu, P., Chen, P., and Li, H., 2014. Effects of low salinity stress on microstructure of gill and hepatopancreas and family survival rate of Portunus trituberculatus. Progress in Fishery Sciences, 35 (1): 1–104 (in Chinese with English abstract).

    Google Scholar 

  • Jiang, Q., Li, Q., Yu, H., and Kong, L., 2016. Inheritance and variation of genomic DNA methylation in diploid and triploid Pacific oyster (Crassostrea gigas). Marine Biotechnology, 18: 124–132.

    Article  Google Scholar 

  • Jiang, Q., Yu, H., Kong, L. F., and Li, Q., 2014. Analysis of DNA methylation in different tissues of the Pacific oyster (Crassostrea gigas) with the fluorescence-labeled methylation-sensitive amplified polymorphism (F-MSAP). Journal of Fishery Sciences of China, 21 (4): 4–676 (in Chinese with English abstract).

    Google Scholar 

  • Kong, N., Liu, X., Li, J. Y., Mu, W. D., Lian, J. W., Xue, Y. J., and Li, Q., 2017. Effects of temperature and salinity on survival, growth and DNA methylation of juvenile Pacific abalone, Haliotis discus hannai Ino. Chinese Journal of Oceanology and Limnology, 35 (5): 5–1248.

    Article  Google Scholar 

  • Koyama, H., Mizusawa, N., Hoashi, M., Tan, E., Yasumoto, K., Jimbo, M., Ikeda, D., Yokoyama, T., Asakawa, S., Piyapattanakorn, S., and Watabe, S., 2018. Changes in free amino acid concentrations and associated gene expression profiles in the abdominal muscle of kuruma shrimp (Marsupenaeus japonicus) acclimated at different salinities. Journal of Experimental Biology, 221: jebl68997.

    Article  Google Scholar 

  • Li, E., Beard, C., and Jaenisch, R., 1993. Role for DNA methylation in genomic imprinting. Nature, 366 (6453): 6453–362.

    Article  Google Scholar 

  • Li, S., He, F., Wen, H., Li, J., Si, Y., Liu, M., He, H., and Huang, Z., 2017. Analysis of DNA methylation level by methylation-sensitive amplification polymorphism in half smooth tongue sole (Cynoglossus semilaevis) subjected to salinity stress. Journal of Ocean University China, 16 (2): 2–269.

    Google Scholar 

  • Maegawa, S., Hinkal, G., Kim, H. S., Shen, F., Li, Z., Zhang, J., Zhang, N., Liang, S., Donehower, L. A., and Issa, J. P., 2010. Widespread and tissue specific age-related DNA methylation changes in mice. Genome Research, 20 (3): 3–332.

    Article  Google Scholar 

  • Mcnamara, J. C., and Faria, S. C., 2012. Evolution of osmoregulatory patterns and gill ion transport mechanisms in the decapod Crustacea: A review. Journal of Comparative Physiology B, 182 (8): 8–997.

    Article  Google Scholar 

  • Navarromartin, L., Vinas, J., Ribas, L., Diaz, N., Gutierrez, A., Croce, L. D., and Piferrer, F., 2011. DNA methylation of the gonadal aromatase (cypl9a) promoter is involved in temperature-dependent sex ratio shifts in the European sea bass. PLoS Genetics, 7 (12): e1002447.

    Article  Google Scholar 

  • Ou, X., Zhang, Y., Xu, C., Lin, X., Zang, Q., Zhuang, T., Jiang, L., Wettstein, D., and Liu, B., 2012. Transgenerational inheritance of modified DNA methylation patterns and enhanced tolerance induced by heavy metal stress in rice (Oryza sativa L). PLoS One, 7 (9): e41143.

    Article  Google Scholar 

  • Pan, L. Q., Zhang, L. J., and Liu, H. Y., 2007. Effects of salinity and pH on ion-transport enzyme activities, survival and growth of litopenaeus vannamei postlarvae. Aquaculture, 273 (4): 4–711.

    Article  Google Scholar 

  • Romano, N., and Zeng, C. S., 2012. Osmoregulation in decapod crustaceans: Implications to aquaculture productivity, methods for potential improvement and interactions with elevated ammonia exposure. Aquaculture, 334: 12–23.

    Article  Google Scholar 

  • Ruscoe, I. M., Shelley, C. C., and Williams, G. R., 2004. The combined effects of temperature and salinity on growth and survival of juvenile mud crabs (Scylla serrata Forskal). Aquaculture, 238 (1-4): 239–247.

    Article  Google Scholar 

  • Shan, X., Wang, X., Yang, G., Wu, Y., Su, S., Li, S., Liu, H., and Yuan, Y., 2013. Analysis of the DNA methylation of maize (Zea mays L.) in response to cold stress based on methyla-tion-sensitive amplified polymorphisms. Journal of Plant Biology, 56 (1): 1–32.

    Article  Google Scholar 

  • Smith, Z. D., and Meissner, A., 2013. DNA methylation: Roles in mammalian development. Nature Reviews Genetics, 14 (3): 3–204.

    Article  Google Scholar 

  • Sun, Y., Hou, R., Fu, X., Sun, C., Wang, S., Wang, C., Li, N., Zhang, L., and Bao, Z., 2014. Genomewide analysis of DNA methylation in five tissues of Zhikong scallop, Chlamys farreri. PLoS One, 9 (1): e86232.

    Article  Google Scholar 

  • Xiong, L. Z., Xu, C. G., Maroof, M. A. S., and Zhang, Q., 1999. Patterns of cytosine methylation in an elite rice hybrid and its parental lines, detected by a methylation-sensitive amplification polymorphism technique. Molecular and General Genetics Mgg, 261 (3): 3–439.

    Article  Google Scholar 

  • Xu, Q., Zhang, Y., Sun, D. X., Wang, Y. C., Tang, S. Q., and Zhao, M., 2011. Analysis of DNA methylation in different chicken tissues with MSAP Hereditas, 33 (6): 620.

    Article  Google Scholar 

  • Zhang, C. Y., Wang, N. N., Zhang, Y. H., Feng, Q. Z., Yang, C. W., and Liu, B., 2013. DNA methylation involved in proline accumulation in response to osmotic stress in rice (Oryza sativa). Genetics and Molecular Research, 12 (2): 2–1269.

    Google Scholar 

  • Zhang, X., Li, Q., Kong, L. F., and Yu, H., 2017. DNA methylation changes detected by methylation-sensitive amplified polymorphism in the Pacific oyster (Crassostrea gigas) in response to salinity stress. Genes & Genomics, 39: 1173–1181.

    Article  Google Scholar 

  • Zhao, Y., Chen, M., Storey, K. B., Sun, K., and Yang, H., 2015. DNA methylation levels analysis in four tissues of sea cucumber Apostichopus japonicus based on fluorescence-labeled methylation-sensitive amplified polymorphism (F-MSAP) during aestivation. Comparative Biochemistry and Physiology B, 181: 26–32.

    Article  Google Scholar 

  • Wang, J., Marowsky, N. C., and Fan, C., 2014. Divergence of gene body DNA methylation and evolution of plant duplicate genes. PLoS One, 9 (10): e110357.

    Article  Google Scholar 

  • Wang, M., Qin, F., Xie, C., Li, W., Yuan, J., Kong, F., Yu, W., Xia, G., and Liu, S., 2014. Induced and constitutive DNA methylation in a salinity-tolerant wheat introgression line. Plant and Cell Physiology, 55 (7): 7–1354.

    Article  Google Scholar 

  • Wang, W. S., Pan, Y. J., Zhao, X. Q., Dwivedi, D., Zhu, L. H., Ali, J., Fu, B. Y., and Li, Z. K., 2011. Drought-induced site-specific DNA methylation and its association with drought tolerance in rice (Oryza sativa L.). Journal of Experimental Botany, 62 (6): 6–1951.

    Article  Google Scholar 

Download references

Acknowledgments

This study was supported by the grants from the National Natural Science Foundation of China (No. 4147 6124), the Natural Science Foundation of Zhejiang Province (No. LY17C190005), the Major Agriculture Program of Ningbo (No. 2017C110007), the Ningbo Science and Technology Project (No. 2016C10037), the Open Fund of Ningbo University (No. xkzscl505) and K C Wong Ma-gana Fund in Ningbo University. We would like to thank Dr. Sarah-Louise Counter Selly (University of Stirling, UK) for her linguistic assistance during the preparation of this manuscript.

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Authors and Affiliations

  1. Key Laboratory of Applied Marine Biotechnology of Ministry of Education, Ningbo University, Ningbo, 315211, China

    Shaokun Lu, Ronghua Li, Tianlong Gao, Chunlin Wang, Changkao Mu & Weiwei Song

  2. Collaborative Innovation Center for Zhejiang Marine High-Efficiency and Healthy Aquaculture, Ningbo University, Ningbo, 315211, China

    Shaokun Lu, Ronghua Li, Tianlong Gao, Chunlin Wang, Changkao Mu & Weiwei Song

  3. Third Institute of Oceanography, State Oceanic Administration, Xiamen, 361001, China

    Weibing Zheng

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  1. Shaokun Lu
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  2. Ronghua Li
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  3. Tianlong Gao
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  4. Chunlin Wang
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  5. Weibing Zheng
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  6. Changkao Mu
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  7. Weiwei Song
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Correspondence to Ronghua Li.

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Lu, S., Li, R., Gao, T. et al. Analysis of DNA Methylation Level of Portunus trituberculatus Subjected to Low Salinity with Methylation-Sensitive Amplification Polymorphism. J. Ocean Univ. China 18, 1158–1162 (2019). https://doi.org/10.1007/s11802-019-4045-9

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  • DOI: https://doi.org/10.1007/s11802-019-4045-9

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