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Genetic and epigenetic variation in mass selection populations of Pacific oyster Crassostrea gigas

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Abstract

Selective breeding often produces an improvement in phenotype. Much of the phenotypic change within a species is a consequence of genetic variation. However, there is growing evidence for phenotypic change even in the absence of DNA sequence polymorphisms, termed epigenetic variation. This study’s goal was to investigate the genetic and epigenetic variation in the mass selection populations of the Pacific oyster (Crassostrea gigas), determine if any correlation exists between the genetic and epigenetic variations. This can serve as a first step in investigating the potential role epigenetic variations have in selective breeding. Amplified fragment length polymorphism analysis and methylation-sensitive amplified polymorphism methodology were used to monitor genetic and epigenetic variation in two populations (the base stock and the third selected generation) from a mass selection line in the Pacific oyster. The correlation between genetic and epigenetic variation was evaluated by Co-Inertia Analysis. The genetic difference was mainly found in the gene frequency shift revealed by the F ST value (0.0151, P < 0.01) and no significant reduction in genetic diversity was detected. The percentage of methylation in C. gigas was 26.4 %. No significant difference was observed on the average state of methylation, but a few bands showed different frequencies between the two populations. Co-Inertia Analysis revealed a significant association between the genetic and epigenetic profiles (P < 0.01).

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Acknowledgments

This study was supported by the grants from 973 Program (2010CB126406), National High Technology Research and Development Program (2012AA10A405-6), and National Natural Science Foundation of China (31072207).

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Authors and Affiliations

  1. Key Laboratory of Mariculture Ministry of Education, Ocean University of China, Qingdao, 266003, China

    Qun Jiang, Qi Li, Hong Yu & Ling-Feng Kong

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  1. Qun Jiang
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  2. Qi Li
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  3. Hong Yu
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  4. Ling-Feng Kong
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Correspondence to Qi Li.

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Jiang, Q., Li, Q., Yu, H. et al. Genetic and epigenetic variation in mass selection populations of Pacific oyster Crassostrea gigas . Genes Genom 35, 641–647 (2013). https://doi.org/10.1007/s13258-013-0114-4

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