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LlHSFA1, a novel heat stress transcription factor in lily (Lilium longiflorum), can interact with LlHSFA2 and enhance the thermotolerance of transgenic Arabidopsis thaliana

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Abstract

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A heat stress transcription factor LlHSFA1 in lily and its relationship with LlHSFA2 was investigated, and its function in enhancing thermotolerance was confirmed by analyzing transgenic Arabidopsis thaliana overexpressed LlHSFA1.

Abstract

A large family of heat stress transcription factors that are involved in the heat stress response in plants can induce the expression of multiple genes related to thermotolerance including heat-shock proteins. In this study, a novel class A1 HSF named LlHSFA1 was isolated from leaves of lily (Lilium longiflorum cv. ‘White Heaven’) using the rapid amplification of cDNA ends technique. Analysis of the deduced amino acid sequence and construction of a phylogenetic tree showed that LlHSFA1 contained five critical domains and motifs and belonged to the A1 family of HSFs. Following the heat treatment of lily leaves, transcription of LlHSFA1 was induced to a varying extent, related to the time of measurement. The induced expression peak of LlHSFA1 occurred prior to that of LlHSFA2, during the early phase of heat stress. Following transient expression of LlHSFA1 in Nicotiana benthamiana, LlHSFA1 was found to be localized in both the nucleus and the cytoplasm. Analysis using bimolecular fluorescence complementation and a yeast two-hybrid assay demonstrated that LlHSFA1 could interact with LlHSFA2. Use of a yeast one-hybrid assay confirmed that LlHSFA1 had transcriptional activation activity. In transgenic Arabidopsis lines overexpressing LlHSFA1 under unstressed conditions, the expression of some putative target genes was up-regulated, in comparison with expression in wild-type plants, and furthermore, the thermotolerance of the transgenic lines was enhanced. Overall, LlHSFA1 was demonstrated to play an important role in the heat stress response of lily and to be a novel candidate gene for application in lily breeding, using genetic modification approaches.

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Abbreviations

AARs:

Amino acid residues

BiFC:

Bimolecular fluorescence complementation

DBD:

DNA-binding domain

GFP:

Green fluorescent protein

HS:

Heat shock/stress

HSE:

Heat shock element

HSF:

Heat stress transcription factor

HSP:

Heat-shock protein

HSR:

Heat stress response

NES:

Nuclear export signal

NLS:

Nuclear localization signal

OD:

Oligomerization domain

ORF:

Open reading frame

qRT-PCR:

Quantitative real-time PCR

RACE:

Rapid amplification of cDNA ends technique

UTR:

Untranslated region

WT:

Wild type

YFP:

Yellow fluorescent protein

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Acknowledgments

Our thanks are due to Prof. Junping Gao (China Agricultural University, China) for kindly offering the modified pCAMBIA1300 vector and BiFC vectors to us. We are also grateful to Xingliang Li, PhD (China Agriculture University, China) for his sincere help and good advice. This work was supported by National Natural Science Foundation of China (No. 30972024) and the Science and Technology Specific Project Foundation of Ministry of Agriculture of China (No. 200903020).

Conflict of interest

The authors declare that they have no conflict of interest.

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Authors and Affiliations

  1. Department of Ornamental Horticulture and Landscape Architecture, China Agricultural University, Yuan Mingyuan Western Road 2#, Beijing, 100193, China

    Benhe Gong, Jin Yi, Jian Wu, Juanjuan Sui, Muhammad Ali Khan, Ze Wu, Xionghui Zhong, Shanshan Seng, Junna He & Mingfang Yi

  2. The High School Affiliated to Beijing Normal University, Pinggu First Branch, Xiao Li Road 1#, Beijing, 101204, China

    Jin Yi

  3. College of Biology, Fuyang Normal College, Qinghe Western Road 100#, Fuyang, 236037, Anhui, China

    Juanjuan Sui

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  1. Benhe Gong
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  2. Jin Yi
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  10. Mingfang Yi
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Correspondence to Mingfang Yi.

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Communicated by Qiao Zhao.

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Gong, B., Yi, J., Wu, J. et al. LlHSFA1, a novel heat stress transcription factor in lily (Lilium longiflorum), can interact with LlHSFA2 and enhance the thermotolerance of transgenic Arabidopsis thaliana . Plant Cell Rep 33, 1519–1533 (2014). https://doi.org/10.1007/s00299-014-1635-2

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