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Effects of short-term heat stress on oxidative damage and responses of antioxidant system in Lilium longiflorum

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Abstract

This paper aims to determine the changes in reactive oxygen species (ROS) and the responses of the lily (Lilium longiflorum L.) antioxidant system to short-term high temperatures. Plants were exposed to three levels of heat stress (37°C, 42°C, 47°C) for 10 h when hydrogen peroxide (H2O2) and superoxide (O −2 ) production rate along with membrane injury indexes, and changes in antioxidants were measured. Compared with the control (20°C), electrolyte leakage and MDA concentration varied slightly after 10 h at 37°C and 42°C, while increased significantly at 47°C. During 10 h at 37°C and 42°C, antioxidant enzyme activities, such as SOD, POD, CAT, APX and GR, were stimulated and antioxidants (AsA and GSH concentrations) maintained high levels, which resulted in low levels of O −2 and H2O2 concentration. However, after 10 h at 47°C, SOD, APX, GR activities and GSH concentration were similar to the controls, while POD, CAT activities and AsA concentration decreased significantly as compared with the control, concomitant with significant increase in O −2 and H2O2 concentrations. In addition, such heat-induced effects on antioxidant enzymes were also confirmed by SOD and POD isoform, as Cu/ZnSOD maintained high stability under heat stress and the intensity of POD isoforms reduced with the duration of heat stress, especially at 47°C. It is concluded that in lily plants, the oxidative damage induced by heat stress was related to the changes in antioxidant enzyme activities and antioxidants.

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Abbreviations

ROS:

Reactive oxygen species

O −2 :

Superoxide radical

H2O2 :

Hydrogen peroxide

ERL:

Relative electrolyte leakage

AsA:

Ascorbic acid

GSH:

Glutathione

SOD:

Superoxide dismutase

POD:

Peroxidase

CAT:

Catalase

APX:

Ascorbate peroxidase

GR:

Glutathione reductase

MDA:

Malondiadeyde

GST:

Glutathione transferase

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Acknowledgements

This work was partly supported by the National Natural Science Fund of China (30471221). Furthermore, we thank for Dr. QH Shi for critically reviewing the manuscript and providing valuable suggestion. We are grateful for editors and reviewers for their valuable suggestion and help.

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Authors and Affiliations

  1. Department of Ornamental Horticulture and Landscape Architecture, China Agricultural University, Yuanmingyuan West Blvd, Beijing, 100094, China

    Hui Yin, Qiuming Chen & Mingfang Yi

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  1. Hui Yin
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  2. Qiuming Chen
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  3. Mingfang Yi
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Correspondence to Mingfang Yi.

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Yin, H., Chen, Q. & Yi, M. Effects of short-term heat stress on oxidative damage and responses of antioxidant system in Lilium longiflorum . Plant Growth Regul 54, 45–54 (2008). https://doi.org/10.1007/s10725-007-9227-6

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  • DOI: https://doi.org/10.1007/s10725-007-9227-6

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