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Heat shock factors in rice (Oryza sativa L.): genome-wide expression analysis during reproductive development and abiotic stress

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Abstract

Plants respond to heat stress by enhancing the expression of genes encoding heat shock protein (HSPs) genes through activation of heat shock factors (HSFs) which interact with heat shock elements present in the promoter of HSP genes. Plant HSFs have been divided into three conserved classes viz A, B and C. In the present study, a detailed analysis has been done of all rice HSFs, along with their spliced variants. Their chromosomal localization reveals that six HSFs are segmentally duplicated and four pairs of these segmentally duplicated HSF encoding genes show pseudo-functionalization. Expression profiling through microarray and quantitative real-time PCR showed that eight OsHsfs express at a higher level during seed development, while six HSFs are up-regulated in all the abiotic stresses studied. The expression of OsHsfA2a gene in particular was greatly stimulated by heat stress in both root and shoot tissues and also during panicle and seed development. OsHsfA3 was found more responsive to cold and drought stress, while OsHsfA7 and OsHsfA9 showed developing seed-specific expression. This study also revealed that spliced variants generally accumulated at a higher level in all the tissues examined. Different hormones/elicitors like ABA, brassinosteroids and salicylic acid also alter OsHsf gene expression. Calcium in combination with heat stress elevated further the level of HSF transcripts. Expression analysis by both microarray and real-time RT-PCR revealed a unique stable constitutive expression of OsHsfA1 across all the tissues and stresses. A detailed in silico analysis involving identification of unidentified domains has been done by MEME-motif tool in their full-length proteins as well as in DNA-binding domains. Analysis of 1 kb putative promoter region revealed presence of tissue-specific, abiotic stress and hormone-related cis-acting elements, correlating with expression under stress conditions.

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Acknowledgments

This work was financially supported by Department of Biotechnology, Government of India. HC and PA thank Council of Scientific and Industrial Research and the University Grants Commission, New Delhi, for the award of research fellowships during the course of this investigation.

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  1. Department of Plant Molecular Biology, University of Delhi, South Campus, Benito Juarez Road, Dhaula Kuan, New Delhi, 110 021, India

    Harsh Chauhan, Neetika Khurana, Pinky Agarwal & Paramjit Khurana

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  1. Harsh Chauhan
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  2. Neetika Khurana
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Correspondence to Paramjit Khurana.

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Communicated by P. Westhoff.

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438_2011_638_MOESM1_ESM.jpg

Supplementary Figure S1 Schematic representation of conserved motifs in rice HSF proteins identified using MEME search tool.

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438_2011_638_MOESM5_ESM.jpg

Supplementary Figure S2 Schematic representation of conserved motifs in DNA binding domain (DBD) of different rice HSF proteins identified using MEME search tool.

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Chauhan, H., Khurana, N., Agarwal, P. et al. Heat shock factors in rice (Oryza sativa L.): genome-wide expression analysis during reproductive development and abiotic stress. Mol Genet Genomics 286, 171–187 (2011). https://doi.org/10.1007/s00438-011-0638-8

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