Skip to main content
SpringerLink
Log in

Application of Marker Assisted Selection for Potato Virus Y Resistance in the University of Wisconsin Potato Breeding Program

  • Short Communication
  • Published:
American Journal of Potato Research Aims and scope Submit manuscript

Abstract

Potato virus Y (PVY) is responsible for the majority of seed potato lot rejections in North America. Some commercial cultivars and breeding lines have major genes for PVY resistance, but their use in North American breeding programs has been limited. Marker assisted selection of PVY resistance can increase the efficiency of identifying resistant plants and integrating resistance into new cultivars. We used markers RYSC3 and YES3-3B, linked to genes Ry adg and Ry sto , respectively, to screen 46 breeding clones and cultivars, and identified 19 resistant clones. Resistant parents were crossed with susceptible parents with good market and agronomic traits and molecular marker analysis of each family showed a 1:1 segregation ratio. The persistence of this segregation ratio over 2 years of selection and the lack of major linkage drag as assessed by a breeder rating indicated that there was no evidence of an association of PVY resistance with undesirable traits.

Resumen

El virus Y de la papa (PVY) es responsable por la mayoría de los rechazos de los lotes de papa para semilla en Norteamérica. Algunas variedades comerciales y líneas de mejoramiento tienen genes mayores para resistencia al PVY, pero es limitado su uso en programas de mejoramiento de Norteamérica. La selección asistida con marcadores de resistencia al PVY puede aumentar la eficiencia en la identificación de plantas resistentes y en la integración de la resistencia a nuevas variedades. Nosotros usamos los marcadores RYSC3 y YES3-3B, ligados a los genes Ryadg y Rysto, respectivamente, para evaluar 46 clones y variedades, e identificamos 19 clones resistentes. Se cruzaron padres resistentes con susceptibles de buenas características agronómicas y de mercado, y el análisis de marcadores moleculares de cada familia mostró una relación de segregación de 1:1. La persistencia de esta relación de segregación en dos años de selección, y la carencia de ligamiento mayor de arrastre evaluada por calificación de un fitomejorador, indicaron que no hubo evidencia de una asociación de la resistencia del PVY con caracteres no deseables.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

References

  • Ayala, L., M. van Ginkel, M. Khairallah, B. Keller, and M. Henry. 2001. Expression of Thinopyrum intermedium-derived Barley yellow dwarf virus resistance in elite bread wheat backgrounds. Phytopathology 91: 55–62.

    Article  CAS  PubMed  Google Scholar 

  • Baldauf, P.M., S.M. Gray, and K.L. Perry. 2006. Biological and serological properties of Potato virus Y isolates in Northeastern United States potato. Plant Disease 90: 559–566.

    Article  Google Scholar 

  • Barone, A. 2004. Molecular marker-assisted selection for potato breeding. American Journal of Potato Research 81: 111–117.

    Article  Google Scholar 

  • Brown, J.K.M. 2002. Yield penalties of disease resistance in crops. Current Opinion in Plant Biology 5: 339–344.

    Article  CAS  PubMed  Google Scholar 

  • Burdon, J.J., and P.H. Thrall. 2003. The fitness costs to plants of resistance to pathogens. Genome Biology 4: 227.

    Article  PubMed Central  PubMed  Google Scholar 

  • Cai, X.K., D.M. Spooner, and S.H. Jansky. 2011. A test of taxonomic and biogeographic predictivity: resistance to Potato virus Y in wild relatives of the cultivated potato. Phytopathology 101: 1074–1080.

    Article  CAS  PubMed  Google Scholar 

  • Carputo, D., and L. Frusciante. 2011. Classical genetics and traditional breeding. In Genetics, genomics and breeding of potato, ed. James M. Bradeen and Chittaranjan Kole, 20–40. Enfield: Science Publishers.

    Chapter  Google Scholar 

  • Celebi-Toprak, F., S.A. Slack, and M.M. Jahn. 2002. A new gene, Ny tbr , for hypersensitivity to Potato virus Y from Solanum tuberosum maps to chromosome IV. Theoretical and Applied Genetics 104: 669–674.

    Article  CAS  PubMed  Google Scholar 

  • Cernák, I., K. Decsi, S. Nagy, I. Wolf, Z. Polgár, G. Gulyás, Y. Hirata, and J. Taller. 2008. Development of a locus-specific marker and localization of the Ry sto gene based on linkage to a catalase gene on chromosome XII in the tetraploid potato genome. Breeding Science 58: 309–314.

    Article  Google Scholar 

  • Cockerham, G. 1970. Genetical studies on resistance to Potato viruses X and Y. Heredity 25: 309–348.

    Article  Google Scholar 

  • Collard, B.C.Y., and D.J. Mackill. 2008. Marker-assisted selection: an approach for precision plant breeding in the twenty-first century. Philosophical Transactions of the Royal Society, B: Biological Sciences 363: 557–572.

    Article  CAS  PubMed Central  Google Scholar 

  • Flis, B., J. Hennig, D. Strzelczyk-Żyta, C. Gebhardt, and W. Marczewski. 2005. The Ry-f sto gene from Solanum stoloniferum for extreme resistant to Potato virus Y maps to potato chromosome XII and is diagnosed by PCR marker GP122718 in PVY resistant potato cultivars. Molecular Breeding 15: 95–101.

    Article  CAS  Google Scholar 

  • Frost, K.E., R.L. Groves, and A.O. Charkowski. 2013. Integrated control of potato pathogens through seed potato certification and provision of clean seed potatoes. Plant Disease 97: 1268–1280.

    Article  Google Scholar 

  • Fulladolsa Palma, A.C., R. Kota, and A.O. Charkowski. 2013. Optimization of a chemiluminescent dot-blot immunoassay for detection of potato viruses. American Journal of Potato Research 90: 306–312.

    Article  CAS  Google Scholar 

  • Gebhardt, C., D. Bellin, H. Henselewski, W. Lehmann, J. Schwarzfischer, and J.P.T. Valkonen. 2006. Marker-assisted combination of major genes for pathogen resistance in potato. Theoretical and Applied Genetics 112: 1458–1464.

    Article  CAS  PubMed  Google Scholar 

  • Le Gouis, J., L. Jestin, F. Ordon, D. de Froidmont, D. Béghin, J.-L. Joseph, and F. Froidmont. 1999. Agronomic comparison of two sets of SSD barley lines differing for the ym4 resistance gene against barley mosaic viruses. Agronomie 19: 125–131.

    Article  Google Scholar 

  • Gray, S., S. De Boer, J. Lorenzen, A. Karasev, J. Whitworth, P. Nolte, R. Singh, A. Boucher, and H. Xu. 2010. Potato virus Y: an evolving concern for potato crops in the United States and Canada. Plant Disease 94: 1384–1397.

    Article  Google Scholar 

  • Halterman, D., A. Charkowski, and J. Verchot. 2012. Potato, viruses, and seed certification in the USA to provide healthy propagated tubers. Pest Technology 6: 1–14.

    Google Scholar 

  • Heldák, J., M. Bežo, V. Štefúnová, and A. Galliková. 2007. Selection of DNA markers for detection of extreme resistance to potato virus Y in tetraploid potato (Solanum tuberosum L.) F1 Progenies. Czech Journal of Genetics and Plant Breeding 43: 125–34.

  • Hosaka, K., Y. Hosaka, M. Mori, T. Maida, and H. Matsunaga. 2001. Detection of a simplex RAPD marker linked to resistance to Potato virus Y in a tetraploid potato. American Journal of Potato Research 78: 191–196.

    Article  CAS  Google Scholar 

  • Jacobsen, E., and H.J. Schouten. 2007. Cisgenesis strongly improves introgression breeding and induced translocation breeding of plants. Trends in Biotechnology 25: 219–223.

    Article  CAS  PubMed  Google Scholar 

  • Karasev, A.V., and S.M. Gray. 2013. Continuous and emerging challenges of Potato virus Y in potato. Annual Review of Phytopathology 51: 571–586.

    Article  CAS  PubMed  Google Scholar 

  • Kasai, K., Y. Morikawa, V.A. Sorri, J.P. Valkonen, C. Gebhardt, and K.N. Watanabe. 2000. Development of SCAR markers to the PVY resistance gene Ry adg based on a common feature of plant disease resistance genes. Genome 43: 1–8.

    Article  CAS  PubMed  Google Scholar 

  • Kerlan, C. 2006. Potato virus Y. CMI/AAB Descriptions of plant viruses 414.

  • Kuhl, J.C. 2011. Mapping and tagging of simply inherited traits. In Genetics, genomics and breeding of potato, ed. James M. Bradeen and Chittaranjan Kole, 90–112. Enfield: Science Publishers.

    Chapter  Google Scholar 

  • Moury, B., B. Caromel, E. Johansen, V. Simon, L. Chauvin, E. Jacquot, C. Kerlan, and V. Lefebvre. 2011. The helper component proteinase cistron of Potato virus Y induces hypersensitivity and resistance in potato genotypes carrying dominant resistance genes on chromosome IV. Molecular Plant-Microbe Interactions 24: 787–797.

    Article  CAS  PubMed  Google Scholar 

  • Ortega, F., and C. Lopez-Vizcon. 2012. Application of molecular marker-assisted selection (MAS) for disease resistance in a practical potato breeding programme. Potato Research 55: 1–13.

    Article  CAS  Google Scholar 

  • Ottoman, R.J., D.C. Hane, C.R. Brown, S. Yilma, S.R. James, A.R. Mosley, J.M. Crosslin, and M.I. Vales. 2009. Validation and implementation of marker-assisted selection (MAS) for PVY resistance (Ry adg gene) in a tetraploid potato breeding program. American Journal of Potato Research 86: 304–314.

    Article  Google Scholar 

  • Plaisted, R.L., D.E. Halseth, B.B. Brodie, S.A. Slack, J.B. Sieczka, B.J. Christ, K.M. Paddock, and M.W. Peck. 2001. Eva: a midseason golden nematode- and virus-resistant variety for use as tablestock or chipstock. American Journal of Potato Research 78: 65–68.

    Article  Google Scholar 

  • R Core Team. 2013. R: A language and environment for statistical computing. Vienna, Austria: R Foundation for Statistical Computing. ISBN 3-900051-07-0, URL http://www.R-project.org/.

  • Rizza, M.D., F.L. Vilaró, D.G. Torres, and D. Maeso. 2006. Detection of PVY extreme resistance genes in potato germplasm from the Uruguayan breeding program. American Journal of Potato Research 83: 297–304.

    Article  Google Scholar 

  • Sagredo, D., B.M.R. Mathias, C.P. Barrientos, I.B. Acuña, J.B. Kalazich, and J. Santos Rojas. 2009. Evaluation of a SCAR RYSC3 marker of the Ry adg gene to select resistant genotypes to Potato virus Y (PVY) in the INIA potato breeding program. Chilean Journal of Agricultural Research 69: 305–315.

    Article  Google Scholar 

  • Sato, M., K. Nishikawa, K. Komura, and K. Hosaka. 2006. Potato virus Y resistance gene, Ry chc , mapped to the distal end of potato chromosome 9. Euphytica 149: 367–372.

    Article  CAS  Google Scholar 

  • Solomon-Blackburn, R.M., and H. Barker. 2001a. A review of host major-gene resistance to potato viruses X, Y, A and V in potato: genes, genetics and mapped locations. Heredity 86: 8–16.

    Article  CAS  PubMed  Google Scholar 

  • Solomon-Blackburn, R.M., and H. Barker. 2001b. Breeding virus resistant potatoes (Solanum tuberosum): a review of traditional and molecular approaches. Heredity 86: 17–35.

    Article  CAS  PubMed  Google Scholar 

  • Solomon-Blackburn, R.M., and J.E. Bradshaw. 2007. Resistance to Potato virus Y in a multitrait potato breeding scheme without direct selection in each generation. Potato Research 50: 87–95.

    Article  Google Scholar 

  • Solomon-Blackburn, R.M., and G.R. Mackay. 1993. Progeny testing for resistance to Potato virus Y: a comparison of susceptible potato cultivars for use in test-crosses with resistant parents. Potato Research 36: 327–333.

    Article  Google Scholar 

  • Song, Y.-S., L. Hepting, G. Schweizer, L. Hartl, G. Wenzel, and A. Schwarzfischer. 2005. Mapping of extreme resistance to PVY (Ry sto ) on chromosome XII using anther-culture-derived primary dihaploid potato lines. Theoretical and Applied Genetics 111: 879–887.

    Article  CAS  PubMed  Google Scholar 

  • Song, Y.-S., and A. Schwarzfischer. 2008. Development of STS markers for selection of extreme resistance (Ry sto ) to PVY and maternal pedigree analysis of extremely resistant cultivars. American Journal of Potato Research 85: 159–170.

    Article  CAS  Google Scholar 

  • Sorri, V.A., K.N. Watanabe, and J.P.T. Valkonen. 1999. Predicted kinase-3a motif of a resistance gene analogue as a unique marker for virus resistance. Theoretical and Applied Genetics 99: 164–170.

    Article  CAS  Google Scholar 

  • Szajko, K., M. Chrzanowska, K. Witek, D. Strzelczyk-Żyta, H. Zagórska, C. Gebhardt, J. Hennig, and W. Marczewski. 2008. The novel gene Ny-1 on potato chromosome IX confers hypersensitive resistance to Potato virus Y and is an alternative to Ry genes in potato breeding for PVY resistance. Theoretical and Applied Genetics 116: 297–303.

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  • Valkonen, J.P.T., S.A. Slack, R.L. Plaisted, K.N. Watanabe. 1994. Extreme resistance is epistatic to hypersensitive resistance to potato virus YO in a Solanum tuberosum ssp. andigena-derived potato genotype. Plant Disease 78: 1177–1180.

  • Valkonen, J.P.T., K. Wiegmann, J.H. Hämäläinen, W. Marczewski, and K.N. Watanabe. 2008. Evidence for utility of the same PCR-based markers for selection of extreme resistance to Potato virus Y controlled by Ry sto of Solanum stoloniferum derived from different sources. Annals 152: 121–130.

    CAS  Google Scholar 

  • Whitworth, J.L., R.G. Novy, D.G. Hall, J.M. Crosslin, and C.R. Brown. 2009. Characterization of broad spectrum Potato virus Y resistance in a Solanum tuberosum ssp. andigena-derived population and select breeding clones using molecular markers, grafting, and field inoculations. American Journal of Potato Research 86: 286–296.

    Article  CAS  Google Scholar 

  • Witek, K., D. Strzelczyk-Żyta, J. Hennig, and W. Marczewski. 2006. A multiplex PCR approach to simultaneously genotype potato towards the resistance alleles Ry-f sto and Ns. Molecular Breeding 18: 273–275.

    Article  CAS  Google Scholar 

Download references

Acknowledgments

We thank our funding sources, the Wisconsin Potato and Vegetable Growers Association, the USDA Specialty Crop Research Initiative Grant project number 2009–02768, and the USDA/NIFA Grant for the NC Potato Breeding Programs. We also thank Bryan Bowen, the Rhinelander Agricultural Research Station staff, and Nicholas Keuler for their assistance in this work.

All the experiments performed complied with the current regulations of the United States of America.

Author information

Authors and Affiliations

  1. Department of Plant Pathology, University of Wisconsin-Madison, 1630 Linden Drive, Madison, WI, 53706, USA

    Ana C. Fulladolsa, Rajitha Kota, Kristi Severson & Amy O. Charkowski

  2. Department of Horticulture, University of Wisconsin-Madison, 1575 Linden Drive, Madison, WI, 53706, USA

    Felix M. Navarro & Jiwan P. Palta

Authors
  1. Ana C. Fulladolsa
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Felix M. Navarro
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Rajitha Kota
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Kristi Severson
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Jiwan P. Palta
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Amy O. Charkowski
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Amy O. Charkowski.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Fulladolsa, A.C., Navarro, F.M., Kota, R. et al. Application of Marker Assisted Selection for Potato Virus Y Resistance in the University of Wisconsin Potato Breeding Program. Am. J. Potato Res. 92, 444–450 (2015). https://doi.org/10.1007/s12230-015-9431-2

Download citation

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s12230-015-9431-2

Keywords

Search

Navigation