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Mapping of extreme resistance to PVY (Ry sto) on chromosome XII using anther-culture-derived primary dihaploid potato lines

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Abstract

The inheritance of extreme resistance to PVY (Ry sto) by a single dominant locus was confirmed by obtaining a 1:1 segregation ratio in a virus inoculation test with 28 resistant (Ryry) to 29 susceptible (ryry) anther culture-derived dihaploid lines (2n=2x=24) from cv. “Assia” (2n=4x=48) having extreme resistance derived from Solanum stoloniferum in simplex constitution (Ryryryry). Twelve Ry sto markers selected in AFLP assays using bulked segregant analysis were applied to 106 tested potato cultivars from Germany, The Netherlands and Poland and 19 potato cultivars were identified by these markers as extremely resistant to PVY in alignment with phenotypic data. The locus for extreme resistance (Ry sto) to PVY was mapped on chromosome XII co-segregating with the SSR marker STM0003. The utility of anther-culture derived dihaploid potatoes for genetic marker development was demonstrated. Marker transferability from diploids to tetraploids provides an optimistic potential for marker-assisted selection in potato breeding programs.

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Acknowledgments

The authors thank Heike Scholz for producing the dihaploid population by anther culture and Josef Schwarzfischer for his information and technical support. Especially, we want to thank the DAAD (Deutscher Akademischer Austauschdienst) for financial support.

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Authors and Affiliations

  1. Bayerische Landesanstalt für Landwirtschaft, Institut für Pflanzenbau und Pflanzenzüchtung, Am Gereuth 8, 85354, Freising, Germany

    Ye-Su Song, Leonard Hepting, Günther Schweizer, Lorenz Hartl & Andrea Schwarzfischer

  2. TUM-Freising Weihenstephan, Wissenschaftszentrum, Am Hochanger 2, 85350, Freising-Weihenstephan, Germany

    Gerhard Wenzel

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  1. Ye-Su Song
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  2. Leonard Hepting
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  3. Günther Schweizer
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  4. Lorenz Hartl
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  5. Gerhard Wenzel
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  6. Andrea Schwarzfischer
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Correspondence to Andrea Schwarzfischer.

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Communicated by C. Möllers

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Song, YS., Hepting, L., Schweizer, G. et al. Mapping of extreme resistance to PVY (Ry sto) on chromosome XII using anther-culture-derived primary dihaploid potato lines. Theor Appl Genet 111, 879–887 (2005). https://doi.org/10.1007/s00122-005-0010-7

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  • DOI: https://doi.org/10.1007/s00122-005-0010-7

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