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The Ry-f sto gene from Solanum stoloniferum for extreme resistant to Potato virus Y maps to potato chromosome XII and is diagnosed by PCR marker GP122718 in PVY resistant potato cultivars

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Abstract

A novel locus for extreme resistance to Potato virus Y (PVY), Ry-f sto , was identified on potato chromosome XII. The gene Ry-f sto has been introgressed from the wild potato species Solanum stoloniferum. Inheritance of Ry-f sto in the tetraploid potato population Rysto was consistent with the model of a single, dominant gene. Bulked segregant analysis identified an ISSR (inter-simple sequence repeat) marker UBC 857980 linked to Ry-f sto . This marker mapped to linkage group XII of a reference potato RFLP (restriction fragment length polymorphism) map. Chromosome XII specific RFLP markers were converted into PCR-based STS and CAPS markers and tested for linkage with Ry-f sto in the population Rysto. CAPS marker GP122718 was tightly linked to the resistance gene and was successfully used to identify Polish and German cultivars expressing extreme resistance to PVY. This indicates that the source of Ry-f sto has been widely utilized in various potato breeding programs and can be monitored by a diagnostic marker in marker-assisted selection.

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Authors and Affiliations

  1. Plant Breeding and Acclimatization Institute, Platanowa 19, 05831, Młochów, Poland

    Bogdan Flis, Danuta Strzelczyk-Żyta & Waldemar Marczewski

  2. Institute of Biochemistry and Biophysics, Polish Academy of Sciences, Pawińskiego 5A, 02106, Warsaw, Poland

    Jacek Hennig

  3. Max-Planck Institute for Plant Breeding Research, Carl von Linne Weg 10, 50829, Cologne, Germany

    Christiane Gebhardt

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  1. Bogdan Flis
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  2. Jacek Hennig
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  3. Danuta Strzelczyk-Żyta
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  4. Christiane Gebhardt
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  5. Waldemar Marczewski
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Correspondence to Waldemar Marczewski.

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Flis, B., Hennig, J., Strzelczyk-Żyta, D. et al. The Ry-f sto gene from Solanum stoloniferum for extreme resistant to Potato virus Y maps to potato chromosome XII and is diagnosed by PCR marker GP122718 in PVY resistant potato cultivars. Mol Breeding 15, 95–101 (2005). https://doi.org/10.1007/s11032-004-2736-3

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  • DOI: https://doi.org/10.1007/s11032-004-2736-3

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