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Marker-assisted combination of major genes for pathogen resistance in potato

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Abstract

Closely linked PCR-based markers facilitate the tracing and combining of resistance factors that have been introgressed previously into cultivated potato from different sources. Crosses were performed to combine the Ry adg gene for extreme resistance to Potato virus Y (PVY) with the Gro1 gene for resistance to the root cyst nematode Globodera rostochiensis and the Rx1 gene for extreme resistance to Potato virus X (PVX), or with resistance to potato wart (Synchytrium endobioticum). Marker-assisted selection (MAS) using four PCR-based diagnostic assays was applied to 110 F1 hybrids resulting from four 2× by 4× cross-combinations. Thirty tetraploid plants having the appropriate marker combinations were selected and tested for presence of the corresponding resistance traits. All plants tested showed the expected resistant phenotype. Unexpectedly, the plants segregated for additional resistance to pathotypes 1, 2 and 6 of S. endobioticum, which was subsequently shown to be inherited from the PVY resistant parents of the crosses. The selected plants can be used as sources of multiple resistance traits in pedigree breeding and are available from a potato germplasm bank.

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Acknowledgements

The authors thank W. Schuchert for help with the field experiment. Part of this work was carried out in the department of plant breeding and yield physiology at the MPI for Plant Breeding Research headed by Francesco Salamini.

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Authors and Affiliations

  1. Department of Plant Breeding and Genetics, Max-Planck Institute for Plant Breeding Research, Carl von Linné Weg 10, 50829, Köln, Germany

    C. Gebhardt, D. Bellin, H. Henselewski & W. Lehmann

  2. Bayerische Landesanstalt für Bodenkultur und Pflanzenbau, P.O. BOX 1641, 85316, Freising, Germany

    J. Schwarzfischer

  3. Department of Plant Biology and Forest Genetics, SLU, P.O. BOX 7080, 75007, Uppsala, Sweden

    J. P. T. Valkonen

  4. Department of Applied Biology, University of Helsinki, P.O. BOX 27, 00014, Helsinki, Finland

    J. P. T. Valkonen

Authors
  1. C. Gebhardt
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  2. D. Bellin
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  3. H. Henselewski
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  4. W. Lehmann
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  5. J. Schwarzfischer
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  6. J. P. T. Valkonen
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Corresponding author

Correspondence to C. Gebhardt.

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Communicated by F. J. Muehlbauer

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Gebhardt, C., Bellin, D., Henselewski, H. et al. Marker-assisted combination of major genes for pathogen resistance in potato. Theor Appl Genet 112, 1458–1464 (2006). https://doi.org/10.1007/s00122-006-0248-8

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  • DOI: https://doi.org/10.1007/s00122-006-0248-8

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