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Development of STS Markers for Selection of Extreme Resistance (Ry sto ) to PVY and Maternal Pedigree Analysis of Extremely Resistant Cultivars

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An Erratum to this article was published on 05 September 2008

Abstract

Two Ry sto STS markers, YES3-3A (341 bp) and YES3-3B (286 bp), were successfully developed from the Ry sto AFLP marker E+ACC/M+CTC-365. Both STS markers identified all 38 extremely resistant potato cultivars out of 188 European potato varieties from Germany, Hungary, Poland and The Netherlands, and also identified four extremely resistant MPI breeding lines out of five tested lines, in agreement with the phenotypic data of resistance level to PVY. In parallel, three different plastid-mitochondrial configurations (W/α, W/γ, and T/β) were analyzed by the PCR markers ALC_1,3 and ALM_4,5. Thirty-eight potato varieties were classified as the W/α cytoplasm type, 51 potato varieties were identified as carrying the W/γ cytoplasm type, and 99 potato varieties analyzed had the T/β cytoplasm type. All 38 marker-selected extremely resistant potato cultivars and four extremely resistant MPI breeding lines were identified as having the mitochondrial γ type in association with male sterility. Male sterility was validated by pollen germination tests in 38 potato cultivars carrying the mitochondrial α type or γ type. The male sterility of 17 extremely resistant potato cultivars and five potato cultivars carrying the W/γ cytoplasm type was confirmed by obtaining scarcely any or only few or wrinkled pollen grains and no subsequent pollen germination. On the other hand, pollen germination in all 16 potato cultivars with the W/α cytoplasm type was observed with different efficiency due to different genotypes. The Ry sto gene of extremely resistant potato cultivars and MPI breeding lines traced back to Solanum stoloniferum, and the maternal pedigree analysis of these cultivars clustered into three groups depending on three different primary crosses with S. stoloniferum-hybrids. This pedigree analysis confirmed the precision of Ry sto STS marker selection tagging the maternally inherited Ry sto gene derived from MPI breeding lines.

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Abbreviations

SSR:

simple sequence repeat

STS:

sequence tagged site

PVY:

potato virus Y

AFLP:

amplified fragment length polymorphism

References

  • Ballvora, A., M.R. Ercolano, J. Weiß, K. Meksem, C.A. Bormann, P. Oberhagemann, F. Salamini, and C. Gebhardt. 2002. The R1 gene for potato resistance to late blight (Phytophthora infestans) belongs to the leucine zipper/NBS/LRR class of plant resistance genes. Plant Journal 30: 361–371.

    Article  PubMed  CAS  Google Scholar 

  • Barker, H. 1997. Extreme resistance to potato virus V in clones of Solanum tuberosum that are also resistant to potato virus Y and A: evidence for a locus conferring broad-spectrum potyvirus resistance. Theoretical and Applied Genetics 95: 1258–1262.

    Article  CAS  Google Scholar 

  • Barone, A. 2004. Molecular marker-assisted selection for potato breeding. American Journal of Potato Research 81: 111–117.

    Google Scholar 

  • Bendahmane, A., M. Querci, K. Kanyuka, and D.C. Baulcombe. 2000. Agrobacterium transient expression system as a tool for the isolation of disease resistance genes: application to the Rx2 locus in potato. Plant Journal 1: 73–81.

    Article  Google Scholar 

  • Beschreibende Sortenliste Kartoffeln. 1979. Bundessortenamt, Hannover.

  • Beschreibende Sortenliste Kartoffeln. 1983. Bundessortenamt, Hannover.

  • Beschreibende Sortenliste Kartoffeln. 1988. Bundessortenamt, Hannover.

  • Beschreibende Sortenliste Kartoffeln. 1993. Bundessortenamt, Hannover.

  • Beschreibende Sortenliste Kartoffeln. 1996. Bundessortenamt, Hannover.

  • Beschreibende Sortenliste Kartoffeln. 2001. Bundessortenamt, Hannover.

  • Beschreibende Sortenliste Kartoffeln. 2003. Bundessortenamt, Hannover.

  • Beschreibende Sortenliste Kartoffeln. 2004. Bundessortenamt, Hannover.

  • Beschreibende Sortenliste Kartoffeln. 2005. Bundessortenamt, Hannover.

  • Beschreibende Sortenliste Kartoffeln. 2006. Bundessortenamt, Hannover.

  • Brugmans, B., R.G.M. Van der Hulst, R.G.F. Visser, P. Lindhout, and H.J. van Eck. 2003. A new and versatile method for the successful conversion of AFLPä markers into simple single locus markers. Nucleic Acids Research 31: e55.

    Article  PubMed  Google Scholar 

  • Cockerham, G. 1943. Potato breeding for virus resistance. Annals of Applied Biology 30: 105–108.

    Article  Google Scholar 

  • Cockerham, G. 1970. Genetic studies in resistance to potato virus X and Y. Heredity 25: 309–348.

    Article  Google Scholar 

  • De Bokx, J.A., and Huttinga, H. 1981. Potato virus Y. CMI/AAB Descr. P1. Viruses no. 242.

  • Flis, B., J. Henning, D. Strzelczyk-Żyta, C. Gebhardt, and W. Marczewski. 2005. The Ry-fsto gene from Solanum stoloniferum for extreme resistance to potato virus Y maps to potato chromosome XII and is diagnosed by PCR marker GP122718 in PVY resistant potato cultivars. Molecular Breeding 15: 95–101.

    Article  CAS  Google Scholar 

  • Galvez, R., and C.R. Brown. 1980. Inheritance of extreme resistance to PVY derived from Solanum tuberosum spp. andigena. American Potato Journal 57: 476–477.

    Google Scholar 

  • Gebhardt, C., and J. Valkonen. 2001. Organization of genes controlling disease resistance in the potato genome. Annual Review of Phytopathology 39: 79–102.

    Article  PubMed  CAS  Google Scholar 

  • Hämäläinen, J.H., K.N. Watanabe, J.P.T. Valkonen, A. Arihara, R.L. Plaisted, E. Pehu, L. Miller, and S.A. Slack. 1997. Mapping and marker-assisted selection for a gene for extreme resistance to potato virus Y. Theoretical and Applied Genetics 94: 192–197.

    Article  Google Scholar 

  • Hämäläinen, J.H., V.A. Sorri, K.N. Watanabe, C. Gebhardt, and J.P.T. Valkonen. 1998. Molecular examination of a chromosome region that controls resistance to potato Y and A potyviruses in potato. Theoretical and Applied Genetics 96: 1036–1043.

    Article  Google Scholar 

  • Hämäläinen, J.H., T. Kekarainen, C. Gebhardt, K.N. Watanabe, and J.P.T. Valkonen. 2000. Recessive and dominant genes interfere with the vascular transport of potato virus A in diploid potatoes. Molecular Plant–Microbe Interactions 13: 402–412.

    Article  PubMed  Google Scholar 

  • Hawkes, J.G. 1992. Biosystematics of the potato. In The Potato Crop The Scientific Basis for Improvement, Ed 2, ed. P.M. Harris, 13–64. London: Capman & Hall.

    Google Scholar 

  • Hosaka, K. 2004. An easy, rapid, and inexpensive DNA extraction method, “One-minute DNA extraction” for PCR in potato. American Journal of Potato Research 81: 17–19.

    Article  CAS  Google Scholar 

  • Hosaka, K., Y. Hosaka, M. Mori, T. Maida, and H. Matsunaga. 2001. Detection of a simplex RAPD marker linked to resistance to potato virus Y in a tetraploid potato. American Potato Journal 78: 191–196.

    CAS  Google Scholar 

  • Huang, S., E. van der Vossen, H. Kuang, V. Vleeshouwers, N. Zhang, T.J.A. Borm, H.J. van Eck, B. Baker, E. Jacobsen, and R.G.F. Visser. 2005. Comparative genomics enabled the isolation of the R3a late blight resistance gene in potato. Plant Journal 42: 251–261.

    Article  PubMed  CAS  Google Scholar 

  • Jacobs, J.M.E., H.J. van Eck, K. Horsman, P.F.P. Arens, B. Verkerk-Bakker, E. Jacobsen, A. Pereira, and W.J. Stiekema. 1996. Mapping of resistance to the potato cyst nematode Globodera rostochiensis from the wild potato species Solanum vernei. Molecular Breeding 2: 51–60.

    Article  CAS  Google Scholar 

  • Jones, R.A.C. 1990. Strain group specific and virus specific hypersensitive reactions to infection with potyviruses in potato cultivars. Annals of Applied Biology 117: 93–105.

    Article  Google Scholar 

  • Kasai, K., Y. Morikawa, V.A. Sorri, J.P.T. Valkonen, C. Gebhardt, and K.N. Watanabe. 2000. Development of SCAR markers to the PVY resistance gene Ry adg based on a common feature of plant disease resistance genes. Genome 43: 1–8.

    Article  PubMed  CAS  Google Scholar 

  • Le Romancer, M., M. Kerlan, and M. Nedellec. 1994. Biological characterization of various geographical isolates of potato virus Y including superficial necrosis on potato tubers. Plant Pathology 43: 138–144.

    Article  Google Scholar 

  • Lössl, A., N. Adler, R. Horn, U. Frei, and G. Wenzel. 1999. Chondriome-type characterization of potato: mt a, b, g, d, e and novel plastid-mitochondrial configurations in somatic hybrids. Theoretical and Applied Genetics 98: 1–10.

    Article  Google Scholar 

  • Lössl, A., M. Götz, A. Braun, and G. Wenzel. 2000. Molecular markers for cytoplasm in potato: male sterility and contribution of different plastid-mitochondrial configurations to starch production. Euphytica 116: 221–230.

    Article  Google Scholar 

  • Mendoza, H.A., E.J. Mihovilovich, and F. Saguma. 1996. Identification of triplex (YYYy) potato virus Y (PVY) immune progenitors derived from Solanum tuberosum ssp. andigena. American Potato Journal 73: 13–19.

    Article  Google Scholar 

  • Milbourne, D., R.C. Meyer, A.J. Collins, L.D. Ramsay, C. Gebhardt, and R. Waugh. 1998. Isolation, characterization and mapping of simple sequence repeat loci in potato. Molecular & General Genetics 259: 233–245.

    Article  CAS  Google Scholar 

  • Muñoz, F.J., R.L. Plaisted, and H.D. Thurston. 1975. Resistance to potato virus Y in Solanum tuberosum spp. andigena. American Potato Journal 52: 107–115.

    Article  Google Scholar 

  • Niewöhner, J., F. Salamini, and C. Gebhardt. 1995. Development of PCR assays diagnostic for RFLP marker alleles closely linked to alleles Gro1 and H1, conferring resistance to the root cyst nematode Globodera rostochiensis in potato. Molecular Breeding 1: 67–78.

    Article  Google Scholar 

  • Paal, J., H. Henselewski, J. Muth, K. Meksem, C.M. Menendez, F. Salamini, A. Ballvora, and C. Gebhardt. 2004. Molecular cloning of the potato Gro1-4 gene conferring resistance to pathotype Ro1 of the root cyst nematode Globodera rostochiensis, based on a candidate gene approach. Plant Journal 38: 285–297.

    Article  PubMed  CAS  Google Scholar 

  • Ponz, F., and G. Bruening. 1986. Mechanisms of resistance to plant viruses. Annual Review of Phytopathology 24: 355–381.

    Article  CAS  Google Scholar 

  • Ross, H. 1952. Studies on mosaic resistance in the potato. Proceedings of conference on potato virus disease, Wageningen, 40–47.

  • Ross, H. 1958. Inheritance of extreme resistance to virus Y in Solanum stoloniferum and its hybrids with Solanum tuberosum. In: Proceedings of 3rd conference on potato virus diseases, Lisse-Wageningen, 204–211.

  • Ross, H. 1986. Potato breeding-problems and perspectives. In: Advances in Plant Breeding. Supplements to Journal of Plant Breed. vol 13. Paul Parey, Berlin and Hamburg.

  • Ruiz de Arcaute, R., S. Isla, and A. Carrasco. 2002. New strategies on breeding for PVY resistance. In: Proceedings of the 15th European Association for Potato Research. Potato today and tomorrow, Supplement I, Hamburg, 16.

  • Saghai-Maroof, M.A., K.M. Soliman, R.A. Jorgensen, and R.W. Allard. 1984. Ribosomal DNA spacer length polymorphisms in barley: Mendelian inheritance, chromosomal location and population dynamics. Proceedings of the National Academy of Sciences of the United States of America 81: 8014–8018.

    Article  PubMed  CAS  Google Scholar 

  • Sambrook, J., E.F. Fritsch, and T. Maniatis (eds.). 1989. Extraction and purification of plasmid DNA. In: Molecular cloning: a laboratory manual, vol 1, Ed 2. 1.21–1.31. New York: Cold Spring Harbor Laboratory Press.

  • Sato, M., K. Nishikawa, K. Komura, and K. Hosaka. 2006. Potato virus Y resistance gene, Ry chc , mapped to the distal end of potato chromosome 9. Euphytica 149: 367–372.

    Article  CAS  Google Scholar 

  • Schweis, A. 1992. Quantitative Klassifizierungs und Segregationsanalysen nach somatischer Hybridisierung und Cybridisierung bei Kartoffeln. Ph.D. Dissertation, Eberhard-Karls Universität Tübingen, Tübingen, Baden-Württemberg, Germany.

  • Solomon-Blackburn, R.M., and H. Barker. 2001. A review of host major gene resistance to potato viruses X, Y, A and V in potato genes, genetics and mapped locations. Heredity 86: 8–16.

    Article  PubMed  CAS  Google Scholar 

  • Song, Y.S. 2004. Genetic marker analysis in potato for extreme resistance (Ry sto ) to PVY and for chip quality after long term storage at 4°C. Technical University Munich, Freising-Weihenstephan, Bayern, Germany.

  • Song, J., J.M. Bradeen, S.K. Naess, J.A. Raasch, S.M. Wielgus, G.T. Haberlach, J. Liu, H. Kuang, S. Austin-Phillips, C.R. Buell, J.P. Helgeson, and J. Jiang. 2003. Gene RB cloned from Solanum bulbocastanum confers broad spectrum resistance to potato late blight. Proceedings of the National Academy of Sciences of the United States of America 100: 9128–9133.

    Article  PubMed  CAS  Google Scholar 

  • Song, Y.S., L. Hepting, G. Schweizer, L. Hartl, G. Wenzel, and A. Schwarzfischer. 2005. Mapping of extreme resistance to PVY (Ry sto ) on chromosome XII using anther-culture-derived primary dihaploid potato lines. Theoretical and Applied Genetics 111: 879–887.

    Article  PubMed  CAS  Google Scholar 

  • Świeżyński 1994. Inheritance of Resistance to Viruses. In Potato Genetics, eds. J. E. Bradshaw, and G. Mackay, 339–363. Wallingford: CAB International.

    Google Scholar 

  • Świeżyński, K.G. Haynes, R.C.B. Hutten, M.T. Sieczka, P. Watts, and E. Zimnoch-Guzowska. 1997. Pedigree of European and north-American potato varieties. In Plant Breeding and Seed Science, supplement vol 41, no 1, ed. H. J. Czembor, et al. Radzików: Plant Breeding and Acclimatization Institute.

    Google Scholar 

  • Valkonen, J.P.T., and S. Somersalo. 1996. Patterns and barriers of cell-to cell movement and lack of systemic spread of tobacco etch potyvirus (TEV-GUS) in Solanum brevidens. Plant Science 113: 221–228.

    Article  CAS  Google Scholar 

  • van der Vossen, E.A., J.N.A.M. Rouppe van der Voort, K. Kanyuka, A. Bendahmane, H. Sandbrink, D.C. Baulcombe, J. Bakker, W.J. Stiekema, and R.M. Klein-Lankhorst. 2000. Homologues of a single resistance-gene cluster in potato confer resistance to distinct pathogens: a virus and a nematode. Plant Journal 23: 567–576.

    Article  PubMed  Google Scholar 

  • van der Vossen, E.A., A. Sikkema, B. te Lintel Hekkert, J. Gros, P. Stevens, M. Muskens, D. Wouters, A. Pereira, W.J. Stiekema, and S. Allefs. 2003. An ancient R gene from the wild species Solanum bulbocastanum confers broad-spectrum resistance to Phytophthora infestans in cultivated potato and tomato. Plant Journal 36: 867–882.

    Article  PubMed  CAS  Google Scholar 

  • van der Vossen, E.A., J. Gros, A. Sikkema, M. Muskens, D. Wouters, P. Wolters, A. Pereira, and S. Allefs. 2005. The Rpi-blb2 gene from Solanum bulbocastanum is an Mi-1 gene homolog conferring broad-spectrum late blight resistance in potato. Plant Journal 44: 208–222.

    Article  PubMed  CAS  Google Scholar 

  • Weidemann, H.L. 1993. Nekrotische Ringsymptome an Kartoffelknollen. Ein neuer Stamm des Kartoffelvirus Y als Ursache. Kartoffelbau 44: 308–309.

    Google Scholar 

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Acknowledgements

The authors thank Josef Schwarzfischer at the potato breeding department of the Bavaria State Research Center (LfL) in Freising, Dr. Klaus Dehmer at the Institute of Plant Genetics and Crop Plant Research (IPK) in Groß Lüsewitz, Dr. Hofferbert at the Böhm-Nordkartoffel breeding station in Ebstof, Dr. Zimnoch-Guzowska at the Plant Breeding and Acclimatization Institute (IHAR) in Radzików, Poland, and Dr. J. Taller at the University of Veszprem, Hungary for generously providing potato material and pedigree information. This research was supported in part by the Bavaria State Ministry of Agriculture and Forestry.

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  1. Bavarian State Research Center for Agriculture, Institute for Crop Science and Plant Breeding, Am Gereuth 8, 85354, Freising, Weihenstephan, Germany

    Ye-Su Song & Andrea Schwarzfischer

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  1. Ye-Su Song
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Correspondence to Andrea Schwarzfischer.

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An erratum to this article can be found at http://dx.doi.org/10.1007/s12230-008-9044-0

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Song, YS., Schwarzfischer, A. Development of STS Markers for Selection of Extreme Resistance (Ry sto ) to PVY and Maternal Pedigree Analysis of Extremely Resistant Cultivars. Am. J. Pot Res 85, 159–170 (2008). https://doi.org/10.1007/s12230-008-9012-8

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