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Effects of ascorbic acid, alpha-tocopherol, and glutathione on microspore embryogenesis in Brassica napus L.

  • Developmental Biology/Morphogenesis
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Abstract

The impact of culture conditions and addition of antioxidants to media on microspore embryogenesis in rapeseed (Brassica napus cv. ‘PF704’) was investigated. Different concentrations of ascorbic acid (0, 5, 10, 20, 50, 100, and 200 mg l−1) and alpha (α)-tocopherol (0, 5, 10, 20, 50, 100, and 200 mg l−1) were evaluated along with two temperature pretreatments (18 d at 30°C; 2 d at 32.5°C followed by 16 d at 30°C). In addition, combinations of reduced glutathione (0, 10, 50, and 100 mg l−1) and ascorbic acid (5 and 10 mg l−1) were tested. Microspore embryogenesis was significantly enhanced using 10 mg l−1 ascorbic acid (334 embryos per Petri dish) compared with untreated cultures (184 embryos per Petri dish) at 30°C. α-Tocopherol (5 and 10 mg l−1) enhanced (312 and 314 embryos per Petri dish, respectively) microspore embryogenesis relative to untreated cultures (213 embryos per Petri dish) at 30°C, although there were no significant differences among cultures treated with 5–50 mg l−1 α-tocopherol. When 50 mg l−1 α-tocopherol was combined with 5 or 10 mg l−1 ascorbic acid, embryogenesis was significantly enhanced (308 and 328 embryos per Petri dish, respectively) relative to other ascorbic acid levels. Moreover, 10 mg l−1 of reduced glutathione and 5 mg l−l ascorbic acid enhanced microspore embryogenesis (335 embryos per Petri dish) compared to cultures without reduced glutathione (275 embryos per Petri dish). Microspore embryogenesis could be improved by adding ascorbic acid, α-tocopherol, and reduced glutathione when the appropriate combination and temperature pretreatment were selected.

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Authors and Affiliations

  1. Department of Agronomy and Plant Breeding, Urmia University, Urmia, Iran

    Maryam Hoseini & Mortaza Ghadimzadeh

  2. Department of Agronomy and Plant Breeding, Mohaghegh Ardabili University, Ardabil, Iran

    Behzad Ahmadi

  3. Miki-cho Post Office, Ikenobe 3011-2, P. O. Box 7, Kagawa-ken, 761-0799, Japan

    Jaime A. Teixeira da Silva

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  1. Maryam Hoseini
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  2. Mortaza Ghadimzadeh
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  3. Behzad Ahmadi
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  4. Jaime A. Teixeira da Silva
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Correspondence to Behzad Ahmadi.

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Hoseini, M., Ghadimzadeh, M., Ahmadi, B. et al. Effects of ascorbic acid, alpha-tocopherol, and glutathione on microspore embryogenesis in Brassica napus L.. In Vitro Cell.Dev.Biol.-Plant 50, 26–35 (2014). https://doi.org/10.1007/s11627-013-9579-8

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