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Impact of paper bridges, activated charcoal, and antioxidants on growth and development of protocorm-like bodies of hybrid Cymbidium

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Abstract

This study aimed to establish whether protocorm-like bodies (PLBs) of hybrid Cymbidium Twilight Moon ‘Day Light’ could be cultured on paper bridges to remove tissue and medium browning and enhance growth. In addition, the effects of two antioxidants (ascorbic acid [AA] and α-tocopherol [AT]) and activated charcoal (AC) on tissue and medium browning were evaluated. Half-PLBs were cultured on Whatman No. 1 paper bridges in 10 ml of medium containing AC, AA, or AT. The production and development of new PLBs was inferior to that on standard agar-based solid medium when half-PLBs were used. The addition of 1 g/l AC, 10 mg/l AA, or 25 mg/l AT did not affect the formation of new PLBs from half-PLBs, but tissue or medium browning was also not observed. Encapsulated PLBs developed from half-PLBs germinated on medium containing AC or antioxidants. This research provides a simple but effective chemical means, through the use of AC or antioxidants, of avoiding tissue or medium browning without negatively impacting the growth and productivity of orchid PLBs. The use of paper bridges, however, significantly reduced PLB-related growth and development parameters relative to agar-based medium.

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Acknowledgments

The author thanks Prof. Michio Tanaka for research support and assistance with photography.

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  1. Jaime A. Teixeira da Silva

    Present address: , Miki-cho Post Office, Ikenobe 3011-2, P. O. Box 7, Miki-cho, Kagawa-Ken, 761-0799, Japan

Authors and Affiliations

  1. Faculty of Agriculture and Graduate School of Agriculture, Kagawa University, Miki-cho, Kagawa, 761-795, Japan

    Jaime A. Teixeira da Silva

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  1. Jaime A. Teixeira da Silva
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Correspondence to Jaime A. Teixeira da Silva.

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Editor: Neftali Ochoa-Alejo

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Teixeira da Silva, J.A. Impact of paper bridges, activated charcoal, and antioxidants on growth and development of protocorm-like bodies of hybrid Cymbidium . In Vitro Cell.Dev.Biol.-Plant 49, 414–420 (2013). https://doi.org/10.1007/s11627-013-9532-x

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  • DOI: https://doi.org/10.1007/s11627-013-9532-x

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