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Stress-related variation in antioxidative enzymes activity and cell metabolism efficiency associated with embryogenesis induction in isolated microspore culture of triticale (x Triticosecale Wittm.)

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Abstract

Isolated microspore cultures of two spring triticale (x Triticosecale Wittm.) cultivars were used to examine the effect of various stress treatments (either high—32°C or low—5°C temperature with or without nitrogen/carbohydrate starvation) applied to excised anthers on the effectiveness of microspore embryogenesis induction. To quantify the effects of pretreatment conditions, the activity of antioxidative enzymes (catalase, peroxidase and superoxide dismutase) together with respiration rate and heat emission were measured. It was observed that heat shock treatment applied as the only one stress factor increased the activity of antioxidative enzymes which suggests intensive generation of reactive oxygen species. Such pretreatment effectively triggered microspore reprogramming but drastically decreased microspore viability. After low temperature treatment, the activity of antioxidative enzymes was similar to the control subjected only with the stress originated from the transfer to in vitro culture conditions. This pretreatment decreased the number of microspores entering embryogenesis but sustained cell viability and this effect prevailed in the final estimation of microspore embryogenesis effectiveness. For both, low- and high-temperature treatments, interaction with starvation stress was beneficial increasing microspore viability (at 5°C) or efficiency of embryogenesis induction (at 32°C). The latter treatment significantly reduced cell metabolic activity. Physiological background of these effects seems to be different and some hypothetical explanations have been discussed. Received data indicate that in triticale, anther preculture conditions could generate oxidative stress and change the cell metabolic activity which could next be reflected in the cell viability and the efficiency of microspore embryogenesis.

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Abbreviations

ABA:

Abscisic acid

CAT:

Catalase

DH:

Doubled haploids

DW:

Dry weight

EDTA:

Ethylenediaminetetraacetic acid

ELS:

Embryo-like structures

H2O2 :

Hydrogen peroxide

KP:

Potassium phosphate buffer

NAA:

α-Naphthaleneacetic acid

PAR:

Photosynthetic active radiation

PEX:

Peroxidase

ROS:

Reactive oxygen species

SOD:

Superoxide dismutase

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Acknowledgments

The research was supported by the project KBN23/E189/SPB/COST/P06/Dz585/2002-2005.

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Authors and Affiliations

  1. Institute of Plant Physiology, Polish Academy of Sciences, Niezapominajek 21, 30-239, Kraków, Poland

    Iwona Żur, Ewa Dubas, Elżbieta Golemiec, Magdalena Szechyńska-Hebda, Gabriela Gołębiowska & Maria Wędzony

  2. Pedagogical University of Kraków, Podchorążych 2, 30-084, Kraków, Poland

    Gabriela Gołębiowska & Maria Wędzony

Authors
  1. Iwona Żur
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  2. Ewa Dubas
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  3. Elżbieta Golemiec
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  4. Magdalena Szechyńska-Hebda
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  5. Gabriela Gołębiowska
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  6. Maria Wędzony
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Correspondence to Iwona Żur.

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Communicated by E. Guiderdoni.

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Żur, I., Dubas, E., Golemiec, E. et al. Stress-related variation in antioxidative enzymes activity and cell metabolism efficiency associated with embryogenesis induction in isolated microspore culture of triticale (x Triticosecale Wittm.). Plant Cell Rep 28, 1279–1287 (2009). https://doi.org/10.1007/s00299-009-0730-2

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  • DOI: https://doi.org/10.1007/s00299-009-0730-2

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