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Association mapping for pre-harvest sprouting tolerance in common wheat (Triticum aestivum L.)

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Abstract

Association mapping was undertaken in common wheat to identify markers associated with pre-harvest sprouting tolerance (PHST). For this purpose, a population of 242 wheat genotypes and 250 SSR markers were used. The population used consisted of diverse germplasm, which carried sufficient phenotypic variation for PHS for conducting association mapping. The population was found to be structured and stratified into 15 sub-populations; the tolerant and moderately tolerant wheat genotypes were distributed in all the sub-populations. This feature of the population along with other information on population structure was used in association mapping using both the available models, the general linear model (GLM) and the mixed linear model (MLM); hopefully, this minimized the rate of false positives. As many as 30 markers were found to be associated with PHST, 26 markers with GLM and 17 markers with MLM; 13 markers were detected using both the approaches. Only eight SSR markers associated with QTL for PHST were such, which were located within the marker intervals that were earlier reported to carry QTLs for PHST. The remaining 22 markers that were found to be associated with PHST could not be associated with any of the genomic regions known to carry QTLs for PHST, which are known to occur on all the 42 chromosome arms of wheat genome.

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Acknowledgments

Thanks are due to DBT, New Delhi, DST (FIST-program), New Delhi and UGC (SAP-DRS Program), New Delhi for providing financial support and facilities to carry out this study. CSIR, New Delhi awarded SRF to RRM. PKG was awarded the position of NASI-Senior Scientist.

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  1. Molecular Biology Laboratory, Department of Genetics & Plant Breeding, Ch. Charan Singh University, Meerut, 250 004, India

    V. Jaiswal, R. R. Mir, A. Mohan, H. S. Balyan & P. K. Gupta

  2. Division of Plant Breeding & Genetics, Sher-e-Kashmir University of Agricultural Sciences & Technology of Jammu (SKUAST-J), Chatha, Jammu, 180 009, India

    R. R. Mir

  3. Department of Crop and Soil Sciences, Washington State University, Pullman, WA, USA

    A. Mohan

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  1. V. Jaiswal
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Jaiswal, V., Mir, R.R., Mohan, A. et al. Association mapping for pre-harvest sprouting tolerance in common wheat (Triticum aestivum L.). Euphytica 188, 89–102 (2012). https://doi.org/10.1007/s10681-012-0713-1

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