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Association mapping of Stagonospora nodorum blotch resistance in modern European winter wheat varieties

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Abstract

Association mapping in populations relevant for wheat breeding has a large potential for validating and fine-mapping QTLs identified in F2- or DH (double haploid)-derived populations. In this study, associations between markers in the region of QSng.sfr-3BS, a major QTL for resistance to Stagonospora nodorum glume blotch (SNG), and SNG resistance were investigated by linkage and association analyses. After increasing marker density in 240 F5:7 recombinant inbred lines (RILs), QSng.sfr-3BS explained 43% of the genetic variance and peaked 0.6 cM proximal from the marker SUN2-3B. Association between SNG resistance and markers mapped in the region of QSng.sfr-3BS was investigated in a population of 44 modern European winter wheat varieties. Two genetically distinct subpopulations were identified within these lines. In agreement with linkage analyses, association mapping by a least squares general linear model (GLM) at marker loci in the region of QSng.sfr-3BS revealed the highest association with SNG resistance for SUN2-3B (p < 0.05). Association mapping can provide an effective mean of relating genotypes to complex quantitative phenotypes in hexaploid wheat. Linkage disequilibrium (r 2) in chromosome 3B extended less than 0.5 cM in 44 varieties, while it extended about 30 cM in 240 RILs, based on 91 SSR and STS marker-pair comparisons. This indicated that the association mapping population had a marker resolution potential at least 390-fold higher compared to the RIL population.

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Acknowledgments

Special thanks to Dr. Flavio Breseghello for helpful comments on the manuscript, and to Prof. Clay Sneller, Dr. Peter Morrell, Dr. Gael Pressoir, Dr. Edward Buckler, Dr. Jianming Yu for suggestions on statistical analyses. This work was supported by the Swiss National Science Foundation for Scientific Research (Grant No. 105620) and by the University of Zürich.

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Author notes

  1. L. Tommasini

    Present address: Department of Botany and Plant Sciences, University of California Riverside, 2150 Batchelor Hall, Riverside, CA, 92521-0124, USA

  2. T. Schnurbusch

    Present address: University of Adelaide, Waite Campus, Australian Centre for Plant Functional Genomics, PMB1, Glen Osmond, SA, 5064, Australia

Authors and Affiliations

  1. Department of Plant Molecular Biology, Institute of Plant Biology, University of Zürich, Zürich, 8008, Switzerland

    L. Tommasini, T. Schnurbusch & B. Keller

  2. Agroscope Changins, Station fédérale de recherches agronomiques, Nyon1, 1260, Switzerland

    D. Fossati & F. Mascher

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  1. L. Tommasini
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Correspondence to B. Keller.

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Communicated by M. Sillanpää.

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Tommasini, L., Schnurbusch, T., Fossati, D. et al. Association mapping of Stagonospora nodorum blotch resistance in modern European winter wheat varieties. Theor Appl Genet 115, 697–708 (2007). https://doi.org/10.1007/s00122-007-0601-6

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  • DOI: https://doi.org/10.1007/s00122-007-0601-6

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