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Association mapping for frost tolerance using multi-parent advanced generation inter-cross (MAGIC) population in faba bean (Vicia faba L.)

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Abstract

A multi-parent advanced generation inter-cross (MAGIC) derived from 11 founder lines in faba bean was used in this study to identify quantitative trait loci (QTL) for frost tolerance traits using the association mapping method with 156 SNP markers. This MAGIC population consists of a set of 189 genotypes from the Göttingen Winter Bean Population. The association panel was tested in two different experiments, i.e. a frost and a hardening experiment. Six morphological traits, leaf fatty acid composition, relative water content in shoots were scored in this study. The genotypes presented a large genetic variation for all traits that were highly heritable after frost and after hardening. High phenotypic significant correlations were established between traits. The principal coordinates analysis resulted in no clear structure in the current population. Association mapping was performed using a general linear model and mixed linear model with kinship. A False discovery rate of 0.20 (and 0.05) was used to test the significance of marker-trait association. As a result, many putative QTLs for 13 morphological and physiological traits were detected using both models. The results reveal that QTL mapping by association analysis is a powerful method of detecting the alleles associated with frost tolerance in the winter faba bean which can be used in accelerating breeding programs.

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Acknowledgments

We thank apl. Prof. Dr. Wolfgang Link and PD. Dr. Wolfgang Ecke, Department of Crop Sciences, Georg-August-Univeristät Göttingen, for their valuable discussions on this work. We would like to acknowledge Prof. Dr. Donal O’sullivan, University of Reading, UK, for providing us with his consensus map (Webb et al. 2015). This work was financially supported by the Cultural Affairs & Mission Sector in Egypt and the German Academic Exchange Service (DAAD).

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The authors declare that they have no competing interests and understand the conditions of ethical responsibilities that are addressed by Genetica.

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  1. Ahmed Sallam

    Present address: Department of Genetics, Faculty of Agriculture, Assiut University, Assiut, 71526, Egypt

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  1. Department of Crop Sciences, Georg-August-Univeristät Göttingen, Von-Siebold-Str. 8, 37075, Göttingen, Germany

    Ahmed Sallam & Regina Martsch

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  1. Ahmed Sallam
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Correspondence to Ahmed Sallam.

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Sallam, A., Martsch, R. Association mapping for frost tolerance using multi-parent advanced generation inter-cross (MAGIC) population in faba bean (Vicia faba L.). Genetica 143, 501–514 (2015). https://doi.org/10.1007/s10709-015-9848-z

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