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Quantitative trait loci for resistance to pre-harvest sprouting in US hard white winter wheat Rio Blanco

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Abstract

Pre-harvest sprouting (PHS) of wheat is a major problem that severely limits the end-use quality of flour in many wheat-growing areas worldwide. To identify quantitative trait loci (QTLs) for PHS resistance, a population of 171 recombinant inbred lines (RILs) was developed from the cross between PHS-resistant white wheat cultivar Rio Blanco and PHS-susceptible white wheat breeding line NW97S186. The population was evaluated for PHS in three greenhouse experiments and one field experiment. After 1,430 pairs of simple sequence repeat (SSR) primers were screened between the two parents and two bulks, 112 polymorphic markers between two bulks were used to screen the RILs. One major QTL, QPhs.pseru-3AS, was identified in the distal region of chromosome 3AS and explained up to 41.0% of the total phenotypic variation in three greenhouse experiments. One minor QTL, QPhs.pseru-2B.1, was detected in the 2005 and 2006 experiments and for the means over the greenhouse experiments, and explained 5.0–6.4% of phenotypic variation. Another minor QTL, QPhs.pseru-2B.2, was detected in only one greenhouse experiment and explained 4.5% of phenotypic variation for PHS resistance. In another RIL population developed from the cross of Rio Blanco/NW97S078, QPhs.pseru-3AS was significant for all three greenhouse experiments and the means over all greenhouse experiments and explained up to 58.0% of phenotypic variation. Because Rio Blanco is a popular parent used in many hard winter wheat breeding programs, SSR markers linked to the QTLs have potential for use in high-throughput marker-assisted selection of wheat cultivars with improved PHS resistance as well as fine mapping and map-based cloning of the major QTL QPhs.pseru-3AS.

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Acknowledgments

Research is partly funded by the National Research Initiative of the USDA Cooperative State Research, Education and Extension Service, Coordinated Agricultural Project grant number 2006-55606-16629. Mention of trade names or commercial products in this article is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the US Department of Agriculture. This is contribution No. 08-216-J from the Kansas Agricultural Experiment Station, Manhattan, KS, USA.

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Authors and Affiliations

  1. Department of Agronomy, Kansas State University, Manhattan, KS, 66506, USA

    Shubing Liu, Shibin Cai & Cuixia Chen

  2. Jiangsu Academy of Agricultural Science, Nanjing, 210014, China

    Shibin Cai

  3. USDA-ARS, 314 Biochem Hall, University of Nebraska, Lincoln, NE, 68583, USA

    Robert Graybosch

  4. USDA-ARS, Plant Science and Entomology Research Unit, 4008 Throckmorton Hall, Manhattan, KS, 66506, USA

    Guihua Bai

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  1. Shubing Liu
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  2. Shibin Cai
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  3. Robert Graybosch
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  4. Cuixia Chen
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  5. Guihua Bai
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Correspondence to Guihua Bai.

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Communicated by A. Charcosset.

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Liu, S., Cai, S., Graybosch, R. et al. Quantitative trait loci for resistance to pre-harvest sprouting in US hard white winter wheat Rio Blanco. Theor Appl Genet 117, 691–699 (2008). https://doi.org/10.1007/s00122-008-0810-7

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