Abstract
Pre-harvest sprouting (PHS) is a complex trait controlled by multiple genes with strong interaction between environment and genotype that makes it difficult to select breeding materials by phenotypic assessment. One of the most important genes for pre-harvest sprouting resistance is consistently identified on the long arm of chromosome 4A. The 4AL PHS tolerance gene has therefore been targeted by Australian white-grained wheat breeders. A new robust PCR marker for the PHS QTL on wheat chromosome 4AL based on candidate genes search was developed in this study. The new marker was mapped on 4AL deletion bin 13-0.59-0.66 using 4AL deletion lines derived from Chinese Spring. This marker is located on 4AL between molecular markers Xbarc170 and Xwg622 in the doubled-haploid wheat population Cranbrook × Halberd. It was mapped between molecular markers Xbarc170 and Xgwm269 that have been previously shown to be closely linked to grain dormancy in the doubled haploid wheat population SW95-50213 × Cunningham and was co-located with Xgwm269 in population Janz × AUS1408. This marker offers an additional efficient tool for marker-assisted selection of dormancy for white-grained wheat breeding. Comparative analysis indicated that the wheat chromosome 4AL QTL for seed dormancy and PHS resistance is homologous with the barley QTL on chromosome 5HL controlling seed dormancy and PHS resistance. This marker will facilitate identification of the gene associated with the 4A QTL that controls a major component of grain dormancy and PHS resistance.
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Abbreviations
- DH:
-
Doubled-haploid
- MAS:
-
Marker-assisted selection
- PHS:
-
Pre-harvest sprouting
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Acknowledgments
We thank Ms Fiona Drake-Brockman and Ms. Esther Walker of Department of Agriculture & Food Western Australia for providing us with the DNA samples of DH population Cranbrook × Halberd, and DNA samples of Chinese Spring derived wheat nullisomic-tetrasomic (NT) lines and deletion bin lines. We thank Dr. Biddulph of Department of Agriculture & Food Western Australia and Dr. Lu of Sydney University for providing us phenotype information for some wheat breeding lines. This work is within the project “Improvement of malting barley pre-harvest sprouting tolerance through molecular techniques” supported by Molecular Plant Breeding Cooperative Research Centre, Australia.
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Zhang, XQ., Li, C., Tay, A. et al. A new PCR-based marker on chromosome 4AL for resistance to pre-harvest sprouting in wheat (Triticum aestivum L.). Mol Breeding 22, 227–236 (2008). https://doi.org/10.1007/s11032-008-9169-3
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DOI: https://doi.org/10.1007/s11032-008-9169-3