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The effects of jasmonic acid and methyl jasmonate on rosmarinic acid production in Mentha × piperita cell suspension cultures

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Abstract

The effects of two elicitors: jasmonic acid and methyl jasmonate on cell growth as well as on rosmarinic acid accumulation in cell suspension cultures of Mentha × piperita were investigated. The highest rosmarinic acid accumulation 117.95 mg g−1 DW (12% DW) was measured 24 h after addition of 100 μM methyl jasmonate. A similar concentration 110.12 mg g−1 DW was detected 48 h after application of 200 μM jasmonic acid. Those values were nearly 1.5 times higher compared to the control sample, without elicitation. There was no substantial influence of elicitors on rosmarinic acid secretion into the culture media. Extracellular concentrations of rosmarinic acid were similar to the values from the control variants. It was documented that suspension cultures of M. piperita treated with elicitors showed a decrease in biomass accumulation when compared to the control.

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Abbreviations

2,4-D:

2,4-Dichlorophenoxyacetic acid

2iP:

6-γ,γ-Dimethylallylaminopurine

JA:

Jasmonic acid

MeJA:

Methyl jasmonate

RA:

Rosmarinic acid

LS:

Linsmayer and Skoog medium

HCOOH:

Formic acid

UPLC:

Ultra performance liquid chromatography

SPE:

Solid phase extraction

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Acknowledgments

The work was performed under the 6th Framework Program of European Union NUTRA-SNACKS project.

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Authors and Affiliations

  1. Department of Biochemistry and Crop Quality, Institute of Soil Science and Plant Cultivation, State Research Institute, ul. Czartoryskich 8, 24-100, Pulawy, Poland

    Justyna Krzyzanowska, Lukasz Pecio, Anna Stochmal & Wieslaw Oleszek

  2. Department of Plant Breeding and Biotechnology, Institute of Soil Science and Plant Cultivation, State Research Institute, ul. Czartoryskich 8, 24-100, Pulawy, Poland

    Anna Czubacka, Marcin Przybys & Teresa Doroszewska

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  1. Justyna Krzyzanowska
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  2. Anna Czubacka
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  3. Lukasz Pecio
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  5. Teresa Doroszewska
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Correspondence to Justyna Krzyzanowska.

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Krzyzanowska, J., Czubacka, A., Pecio, L. et al. The effects of jasmonic acid and methyl jasmonate on rosmarinic acid production in Mentha × piperita cell suspension cultures. Plant Cell Tiss Organ Cult 108, 73–81 (2012). https://doi.org/10.1007/s11240-011-0014-8

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