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Effects of abiotic and biotic elicitors on growth and isoflavonoid accumulation in Pueraria candollei var. candollei and P. candollei var. mirifica cell suspension cultures

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Abstract

This study demonstrates the effects of various concentrations of abiotic and biotic elicitors on the cell growth and isoflavonoid accumulation of P. candollei var. mirifica (PM) and P. candollei var. candollei (PC) cell suspension cultures. The two plant varieties exhibited different growth responses and varied isoflavonoid accumulation after the addition of elicitors. Copper sulfate, methyl jasmonate (MeJA), and yeast extract did not significantly affect the growth of either plant variety, whereas oligosaccharide and the biotic elicitors used in this study [i.e., 50 mg l−1 chitosan and all concentrations of laminarin (LAM)] suppressed the growth of PM. The addition of MeJA to the medium principally induced an effect on the isoflavonoid content in both PM and PC, with 2.0 μM MeJA inducing the highest isoflavonoid content, as indicated by the induction index—4.41 in PM and 9.62 in PC cells on the 12th and ninth day of culture, respectively. A maximum total isoflavonoid content of 40.49 mg g−1 dry weight was achieved in PM 21 days after elicitation with 2.0 μM MeJA. LAM elicited the PM cell suspension culture to produce puerarin, which was not found in the unelicited culture. The results of this study provide information that will be useful for enhancing the accumulation of isoflavonoids in P. candollei cell suspension cultures.

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Abbreviations

BA:

6-Benzyladenine

CHI:

Chitosan

CuSO4 :

Copper sulfate

2,4-D:

2,4-Dichlorophenoxyacetic acid

II index:

Isoflavonoid induction index

JA:

Jasmonic acid

LAM:

Laminarin

MeJA:

Methyl jasmonate

PC:

P. candollei var. candollei

PM:

P. candollei var. mirifica

YE:

Yeast extract

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Acknowledgments

This work was supported by The Thailand Research Fund (DBG4980009). SK is grateful for financial support from The Royal Golden Jubilee Ph.D. Program (PHD/0143/2548).

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Authors and Affiliations

  1. Department of Pharmaceutical Botany, Faculty of Pharmacy, Mahidol University, 447 Sri-ayuthaya Road, Bangkok, 10400, Thailand

    Sirintra Korsangruang, Promchit Saralamp & Sompop Prathanturarug

  2. Department of Pharmacognosy, Faculty of Pharmacy, Mahidol University, 447 Sri-ayuthaya Road, Bangkok, 10400, Thailand

    Noppamas Soonthornchareonnon

  3. Department of Botany, Faculty of Science, Chulalongkorn University, Bangkok, Thailand

    Yupyn Chintapakorn

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  1. Sirintra Korsangruang
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  2. Noppamas Soonthornchareonnon
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Correspondence to Sompop Prathanturarug.

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Korsangruang, S., Soonthornchareonnon, N., Chintapakorn, Y. et al. Effects of abiotic and biotic elicitors on growth and isoflavonoid accumulation in Pueraria candollei var. candollei and P. candollei var. mirifica cell suspension cultures. Plant Cell Tiss Organ Cult 103, 333–342 (2010). https://doi.org/10.1007/s11240-010-9785-6

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