Abstract.
Two near full-length cDNA clones (LEC4H-1 and LEC4H-2) encoding plant cytochrome P450 were isolated by screening a cDNA library constructed from shikonin-producing Lithospermum erythrorhizon callus cultures. The deduced amino acid sequences of LEC4H-1 and LEC4H-2 exhibited 91% identity and were highly homologous to various cinnamic acid 4-hydroxylase (C4H) sequences from higher plants. Expression studies in yeast confirmed that the P450 clones encode C4H. The level of C4H transcripts in the cultured cells was unaffected by environmental factors, including light irradiation, supply of auxin and methyl jasmonate addition, indicating that transcription of these genes is constitutive and not involved in the regulation of shikonin and rosmarinic acid biosynthesis by these factors.
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Revision received: 15 May 2001
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Yamamura, Y., Ogihara, Y. & Mizukami, H. Cinnamic acid 4-hydroxylase from Lithospermum erythrorhizon: cDNA cloning and gene expression. Plant Cell Rep 20, 655–662 (2001). https://doi.org/10.1007/s002990100373
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DOI: https://doi.org/10.1007/s002990100373