Skip to main content

Advertisement

SpringerLink
Log in

Production of bakuchiol by in vitro systems of Psoralea drupacea Bge

  • Research Note
  • Published:
Plant Cell, Tissue and Organ Culture (PCTOC) Aims and scope Submit manuscript

Abstract

In vitro production of the meroterpene bakuchiol by Psoralea drupacea Bge (Fabaceae) has been studied using aseptically-grown plants, callus cultures of different origin, cell suspensions and transgenic hairy root cultures. The effect of phytohormones and methyl jasmonate on bakuchiol production was also investigated. Bakuchiol was not detected in cell suspensions or hairy root preparations of P. drupacea. In contrast, aerial parts of P. drupacea grown in vitro were found to accumulate up to 11% dry weight of bakuchiol and can therefore be regarded as a potentially useful source of this antimicrobial compound.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1

Abbreviations

BA:

6-Benzyladenine

HPLC:

High-performance liquid chromatography

kin:

Kinetin

LB:

Luria-Bertani medium

MIC:

Minimum inhibitory concentration

MS:

Murashige and Skoog medium

NAA:

1-Naphthaleneacetic acid

TDZ:

Thidiazuron

2,4-D:

2,4-Dichlorophenoxyacetic acid

References

  • Abhyankar G, Reddy VD, Giri CC, Rao KV, Lakshmi VV, Prabhakar S, Vairamani M, Thippeswamy BS, Bhattacharya PS (2005) Amplified fragment polymorphism and metabolomic profiles of hairy roots of Psoralea corylifolia L. Phytochem 66:2441–2457

    Article  CAS  Google Scholar 

  • Adhikari S, Joshi R, Patro BS, Ghanty TK, Chintalwar GJ, Sharma A, Chattopadhyay S, Mukherjee T (2003) Antioxidant activity of bakuchiol: experimental evidence and theoretical treatments on the possible involvement of the terpenoid chain. Chem Res Toxicol 16:1062–1069

    Article  CAS  PubMed  Google Scholar 

  • Backhouse CN, Delporte CL, Negrete RE, Erazo S, Zuniga A, Pinto A, Cassels BK (2001) Active constituents isolated from Psoralea glandulosa L. with anti-inflammatory and antipyretic activities. J Ethnopharmacol 78:27–31

    Article  CAS  PubMed  Google Scholar 

  • Baskaran P, Jayabalan N (2008) Effect of growth regulators on rapid micropropagation and psoralen production in Psoralea corylifolia L. Acta Physiol Plant 30:345–351

    Article  CAS  Google Scholar 

  • Baskaran P, Jayabalan N (2009) Psoralen production in hairy roots and adventitious root cultures of Psoralea corylifolia. Biotechnol Lett 31:1073–1077

    Article  CAS  PubMed  Google Scholar 

  • Bondarenko A, Aizenman B, Zatula D, Bakina L, Kozhina I, Prykhodko V, Silina O, Meshcheryakov A (1977) Psoralea drupacea as a promising source of the antibiotic bakuchiol. Rastit Res 13:460–463

    CAS  Google Scholar 

  • Bouque V, Bourgaud F, Nguyen C, Guckert A (1998) Production of daidzein by callus cultures of Psoralea species and comparison with plants. Plant Cell Tissue Organ Culture 53:35–40

    Article  CAS  Google Scholar 

  • Bourgaud F, Bouque V, Guckert A (1999) Production of flavonoids by Psoralea hairy root cultures. Plant Cell Tissue Organ Culture 56:97–104

    Article  CAS  Google Scholar 

  • Chen H, Li Y (2008) Simple and convenient synthesis of (±)-bakuchiol. Lett Org Chem 5(3):467–469

    Article  CAS  Google Scholar 

  • Danilevsky N, Smirnov V, Prykhodko V, Kosovich T (1985) Sensibility of aerobic microflora of the oral cavity to antibiotic bakuchiol. Stomatology 20:9–12

    Google Scholar 

  • Du XL, Chen HL, Feng HJ, Li YCh (2008) Stereoselective total synthesis of natural (S)-bakuchiol and its enantiomer. Helvetica Chimica Acta 91:371–378

    Article  CAS  Google Scholar 

  • Esumi T, Shimizu H, Kashiyama A, Sasaki C, Toyota M, Fukuyama Y (2008) Efficient construction of a chiral all-carbon quaternary center by asymmetric 1, 4-addition and its application to total synthesis of (+)-bakuchiol. Tetrahedron Lett 49:6846–6849

    Article  CAS  Google Scholar 

  • Fasail M, Anis M (2006) Thidiazuron induced high frequency axillary shoot multiplication in Psoralea corylifolia. Biol Plant 50:437–440

    Article  Google Scholar 

  • Katsura H, Tsukiyama RI, Suzuki A, Kobayashi M (2001) In vitro activities of bakuchiol against oral microorganisms. Antimicrob Agents Chemother 45:3009–3013

    Article  CAS  PubMed  Google Scholar 

  • Lichtenthaler HK, Rohmer M, Schwender J (1997) Two independent biochemical pathways for isopentenyl diphosphate and isoprenoid biosynthesis in higher plants. Physiol Plant 101:643–652

    Article  CAS  Google Scholar 

  • Mehta G, Nayak UR, Dev S (1973) Meroterpenoids. 1. Psoralea corylifolia Linn. 1. Bakuchiol, a novel monoterpene phenol. Tetrahedron 29:1119–1125

    Article  CAS  Google Scholar 

  • Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol Plant 15:473–497

    Article  CAS  Google Scholar 

  • Newton S, Lau C, Gurcha SS, Besra GS, Wright CW (2002) The evaluation of forty-three plant species for in vitro antimicrobial activities. Isolation of active constituents from Psoralea corylifolia and Sanguinaria canadensis. J Ethnopharmacol 79:57–67

    Article  PubMed  Google Scholar 

  • Nguyen C, Bourgaud F, Forlot P, Guckert A (1992) Establishment of hairy root cultures of Psoralea species. Plant Cell Rep 11:424–427

    Article  Google Scholar 

  • Prykhodko V, Bondarenko A (1979) Study of the antimicrobial properties of bakuchiol in in vitro experiments. Mikrobiol Zh (Kiev) 41:400–403

    Google Scholar 

  • Prykhodko V, Bondarenko A, Mishenkova E (1980) Antimicrobial activity and toxicity of the antibiotic bakuchiol. Mikrobiol Zh (Kiev) 42:646–650

    Google Scholar 

  • Prykhodko V, Zhmurko L, Bobyr A, Meshcheryakov A (1982) Bakuchiol activity against tobacco mosaic virus. Mikrobiol Zh (Kiev) 44:73–74

    Google Scholar 

  • Prykhodko V, Kosovich T, Danilevsky N (1984) A study on the possible use of the antibiotic bakuchiol in stomatology. In: VI Congress of the Ukrainian Microbiological Society (Donetsk, June 1984), Part 2, p 151

  • Qiao CF, Han QB, Song JZ, Mo SF, Kong LD, Kung HF, Xu HX (2007) Chemical fingerprint and quantitative analysis of Fructus Psoraleae by high-performance liquid chromatography. J Sep Sci 30:813–818

    Article  CAS  PubMed  Google Scholar 

  • Roberts SC (2007) Production and engineering of terpenoids in plant cell culture. Nat Chem Biol 3:387–395

    Article  CAS  PubMed  Google Scholar 

  • Rout GR, Samantaray S, Das P (2000) In vitro rooting of Psoralea corylifolia Linn: Peroxidase activity as a marker. Plant Growth Reg 30:215–219

    Article  CAS  Google Scholar 

  • Ryu SY, Choi SU, Lee CO, Zee OP (1992) Antitumour activity of Psoralea corylifolia. Arch Pharm Res 15:356–359

    Article  CAS  Google Scholar 

  • Shinde AN, Malpathak N, Fulzele DP (2009) Studied enhancement strategies for phytoestrogen production in shake flasks by suspension culture of Psoralea corylifolia. Biores Technol 100:1833–1839

    Article  CAS  Google Scholar 

Download references

Acknowledgments

This work is part of the project “Establishment and Application of Cell Cultures—Producers of Plant Antimicrobial and Antifungal Substances” (0107U004770) supported by the National Academy of Science of Ukraine.

Author information

Authors and Affiliations

  1. Institute of Cell Biology and Genetic Engineering, National Academy of Sciences of Ukraine, Zabolotnogo str., 148, 03680, Kiev, Ukraine

    Kateryna Lystvan, Valeria Belokurova, Yuriy Sheludko, Olena Kishchenko, Evgenija Paton & Mykola Kuchuk

  2. Plantech (UK), P.O. Box 2104, Reading, RG1 5WG, UK

    John L. Ingham

  3. Institute of Microbiology and Virology, National Academy of Sciences of Ukraine, Zabolotnogo str., 154, 03680, Kiev, Ukraine

    Valeria Prykhodko

Authors
  1. Kateryna Lystvan
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Valeria Belokurova
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Yuriy Sheludko
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. John L. Ingham
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Valeria Prykhodko
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Olena Kishchenko
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. Evgenija Paton
    View author publications

    You can also search for this author in PubMed Google Scholar

  8. Mykola Kuchuk
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Kateryna Lystvan.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Lystvan, K., Belokurova, V., Sheludko, Y. et al. Production of bakuchiol by in vitro systems of Psoralea drupacea Bge. Plant Cell Tiss Organ Cult 101, 99–103 (2010). https://doi.org/10.1007/s11240-009-9657-0

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s11240-009-9657-0

Keywords

Search

Navigation