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A comprehensive study on dehydration-induced antioxidative responses during germination of Indian bread wheat (Triticum aestivum L. em Thell) cultivars collected from different agroclimatic zones

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Abstract

To explore the adaptability of bread wheat to dehydration stress, we screened 28 cultivars collected from different agroclimatic zones, on the basis of malonaldehyde content as biochemical marker in roots of wheat seedlings during germination and classified them as highly tolerant, tolerant, sensitive and highly sensitive. From this primary screening, ten cultivars that showed differential responses to dehydration stress were selected to understand the biochemical and physiological basis of stress tolerance mechanisms. The highly tolerant cultivars showed lower levels of lipid peroxidation, less membrane damage, increased levels of antioxidants, enzymes like catalase, ascorbate peroxidase, glutathione reductase activities, and maintained higher relative water content in comparison to sensitive cultivars, indicating better protection mechanism operating in tolerant cultivars. Correspondingly, highly tolerant cultivars exhibited more accumulation of proline and less H2O2 content across different time points of polyethylene glycol treatments in comparison to sensitive ones. The above biochemical and physiological parameters were further validated through northern analysis of catalase (CAT1) gene, that showed differential expression patterns in tolerant and sensitive cultivars largely in confirmation with the biochemical and physiological analyses. Our study positively correlates the differences in the redox status and antioxidant defense system between tolerant and sensitive cultivars for the establishment of wheat seedlings in typical dehydration conditions.

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Acknowledgements

We are thankful to Vice-Chancellor of the Banasthali University for providing necessary facilities.

Conflict of interest

The authors declare that they have no conflict of interest.

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Authors and Affiliations

  1. Department of Bioscience and Biotechnology, Banasthali University, Banasthali, 304022, Rajasthan, India

    Bharti Garg, Shrilekha Misra & Bhumi Nath Tripathi

  2. Department of Genetics & Plant Breeding, GBPUAT, Pantnagar, 263145, India

    Jai P. Jaiswal

  3. National Institute of Plant Genome Research (NIPGR), Aruna Asaf Ali Marg, JNU Campus, New Delhi, 110067, India

    Manoj Prasad

Authors
  1. Bharti Garg
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  2. Jai P. Jaiswal
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  3. Shrilekha Misra
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  4. Bhumi Nath Tripathi
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  5. Manoj Prasad
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Correspondence to Manoj Prasad.

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Supplementary Table S1

Description of 28 cultivars of wheat used in the present study. The values (a, b, c and d) of lipid peroxidation obtained in Control, 1, 12 and 24 h of dehydration stress in 20 % polyethylene glycol (PEG-6000) respectively expressed as μmole g−1 FW MDA concentration. Standard deviations were calculated from three independent experiments (n = 3). (DOC 84 kb)

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Garg, B., Jaiswal, J.P., Misra, S. et al. A comprehensive study on dehydration-induced antioxidative responses during germination of Indian bread wheat (Triticum aestivum L. em Thell) cultivars collected from different agroclimatic zones. Physiol Mol Biol Plants 18, 217–228 (2012). https://doi.org/10.1007/s12298-012-0117-7

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