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Lipid peroxidation and superoxide dismutase activity in relation to photoinhibition induced by chilling in moderate light

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Abstract

The effect of a chilling stress, at a moderate photon flux density for a few hours, on the peroxidation of membrane lipids and on superoxide dismutase (SOD) activity was compared in leaf slices of chilling-sensitive and chilling-insensitive plants. The aim was to determine if susceptibility to chill-temperature photoinhibition could be related to either damage to membrane lipids by superoxide and-or a decrease in activity of chloroplast SOD. Plants used were Nerium oleander L., grown at 45° C, and Cucumis sativus L., both susceptible to chill-temperature photoinhibition, and N. oleander, grown at 20° C and Spinacia oleracea L., both insensitive to chill-temperature photoinhibition. Lipid peroxidation was assessed by measuring the concentration of malondialdehyde (MDA). Leaf slices from all plants showed a basal level of MDA which decreased by about 15% when the leaf slices were chilled in the light. The level of MDA was not increased by the addition of either KHCO3 or methyl viologen during chilling but it was increased, up to threefold, by the addition of Rose Bengal, which produces singlet oxygen. Chloroplast SOD activity was assessed in leaf extracts as the cyanide-sensitive production of H2O2 in a system which produced superoxide. Activity of SOD was similar in all the plants and was altered little by chilling. The results show that for the plants tested, chilling at a moderate photon flux density for 5 h does not increase the susceptibility of cell membranes to peroxidative damage nor does it decrease the activity of SOD. It was concluded that the susceptibility of chilling-sensitive plants to chill-temperature photoinhibition cannot be explained on the basis of differences in the vulnerability of membrane lipids to damage by superoxide or differences in SOD activity.

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Abbreviations

Chl:

chlorophyll

MDA:

malondialdehyde

MV:

methyl viologen

O -2 :

superoxide

20°-oleander:

Nerium oleander grown at 20° C

45°-oleander:

N. oleander grown at 45° C

PFD:

photon flux density

SOD:

superoxide dismutase

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Author notes

  1. Richard A. J. Hodgson

    Present address: Department of Crop Protection, Waite Agricultural Research Institute, 5064, Glen Osmond, S.A., Australia

Authors and Affiliations

  1. School of Biological Sciences, Macquarie University, 2109, North Ryde, N.S.W., Australia

    Richard A. J. Hodgson

  2. CSIRO Division of Food Processing and School of Biological Sciences, Macquarie University, 2109, North Ryde, N.S.W., Australia

    John K. Raison

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  1. Richard A. J. Hodgson
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  2. John K. Raison
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Hodgson, R.A.J., Raison, J.K. Lipid peroxidation and superoxide dismutase activity in relation to photoinhibition induced by chilling in moderate light. Planta 185, 215–219 (1991). https://doi.org/10.1007/BF00194063

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