Abstract
Postharvest yellowing is accompanied by the rapid nutrition deterioration of broccoli. Here, we investigated the effect of 24-epibrassinolide (EBR) treatment on the change of glucosinolates content during the yellowing of broccoli. EBR treatment maintained the content of glucosinolates, as indicated by higher levels of glucoraphanin, glucobrassicin, and neoglucobrassicin. Meanwhile, the transcript levels of BoCYP83A1 and BoCYP83B1 as well as BoMYB28, BoMYB34, and BoMYB122 involved in the glucosinolates biosynthesis pathway were upregulated in the treatment sample. Furthermore, the expressions of BoBRI1 gene and crucial transcription factors BoBZR1 and BoBES1 that participated in the brassinosteroids signal transduction pathway were activated under EBR treatment, which indirectly alleviated the decrease of glucosinolates content. Orthogonal partial least square model and pathway analysis further demonstrated that the improvement of glucosinolate biosynthetic ability and the enhancement of brassinosteroids signal transduction might be the mechanism responsible for the conservation of glucosinolates by EBR treatment.
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The datasets generated during and/or analyzed during the current study are available from the corresponding author on reasonable request.
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This work was supported by the National Key R&D Program of China [grant number 2016YFD0400103].
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Qingxi Yang: investigation, data curation, formal analysis, writing—original draft. Manli Luo: conceptualization, supervision, visualization. Qian Zhou: conceptualization, resources. Xin Zhou: investigation, conceptualization. Yingbo Zhao: resources, validation. Jianye Chen: conceptualization, resources. Shujuan Ji: project administration, funding acquisition, writing—review and editing.
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Yang, Q., Luo, M., Zhou, Q. et al. Insights into Profiling of 24-Epibrassinolide Treatment Alleviating the Loss of Glucosinolates in Harvested Broccoli. Food Bioprocess Technol 15, 2697–2711 (2022). https://doi.org/10.1007/s11947-022-02909-x
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DOI: https://doi.org/10.1007/s11947-022-02909-x