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In vitro biotechnological approaches on Vanilla planifolia Andrews: advancements and opportunities

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Abstract

In vitro biotechnological advancement of Vanilla plays a major role in germplasm conservation, genetic engineering, accelerated clonal multiplication and production of disease-free plants with enviable aromatic properties. Several attempts have been taken place for the establishment of efficient in vitro protocol for Vanilla in the past few decades. Optimization of various conditions during different phases of micropropagation, for instance development of in vitro aseptic cultures, multiple shoot regeneration, rooting and acclimatization of the plantlets are discussed in this review. In addition to basic micropropagation techniques, various other in vitro biotechnological applications such as clonal fidelity assessment, genetic transformation, synthetic seed technology and cryopreservation are also highlighted. Apart from the existing data, applied aspects like embryo rescue, mutation breeding, genetic engineering, protoplast fusion, somaclonal variation, in vitro enhancement of vanillin production through cell suspension culture, hairy root culture or bioreactors and cryopreservation need to be investigated further. Overall, the current review gives a synopsis on progress and prospect of in vitro culture of Vanilla.

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Fig. 1

Source: https://en.wikipedia.org/wiki/Vanilla_(genus)

Fig. 2
Fig. 3

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Abbreviations

2,4-d :

2,4-dichlorophenoxy acetic acid

BA:

N 6-benzyladenine

Ca:

Callus

GA3 :

Gibberellin A3

IAA:

Indole-3-acetic acid

IBA:

Indole-3-butyric acid

Kinetin:

6-furfurylaminopurine

MS:

Murashige and Skoog (1962)

Mult Sht:

Multiple shoot

NAA:

α-napthalene acetic acid

PGR:

Plant growth regulator

RH:

Relative humidity

Rt:

Root

Sht Reg:

Adventitious shoot regeneration

TDZ:

N-phenyl-N′-(1,2,3-thidiazol-5-yl) urea or thidiazuron

Zeatin:

4-hydroxy-3-methyl-terms-2-butenyl aminopurine

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Acknowledgements

Authors acknowledge the experimental and library support from Department of Agricultural Biotechnology, Faculty of Agriculture, and Directorate of Research, Bidhan Chandra Krishi Viswavidyalaya, West Bengal, India.

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Authors and Affiliations

  1. All India Coordinated Research Project on Groundnut, Directorate of Research, Bidhan Chandra Krishi Viswavidyalaya, Kalyani, Nadia, West Bengal, 741235, India

    Saikat Gantait

  2. Department of Genetics and Plant Breeding, Faculty of Agriculture, Bidhan Chandra Krishi Viswavidyalaya, Mohanpur, Nadia, West Bengal, 741252, India

    Saikat Gantait

  3. Department of Agricultural Biotechnology, Faculty of Agriculture, Bidhan Chandra Krishi Viswavidyalaya, Mohanpur, Nadia, West Bengal, 741252, India

    Suprabuddha Kundu

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Communicated by M. Capuana.

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Gantait, S., Kundu, S. In vitro biotechnological approaches on Vanilla planifolia Andrews: advancements and opportunities. Acta Physiol Plant 39, 196 (2017). https://doi.org/10.1007/s11738-017-2462-1

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  • DOI: https://doi.org/10.1007/s11738-017-2462-1

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