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Direct somatic embryogenesis and genetic homogeneity assessment of regenerated plants of Anthurium andraeanum Linden cv. Fantasia

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An Erratum to this article was published on 20 July 2016

Abstract

We present here a method for the in vitro propagation of Anthurium andraeanum cv. Fantasia through direct somatic embryogenesis. The embryos were directly formed at the cut end of most of the leaf explants when cultured on MS basal medium supplemented with N6-benzyladenine (BA) plus α-naphthalene acetic acid (NAA). MS basal media supplemented with BA (0.27 μM) and NAA (2.68 μM) induced maximum number of somatic embryos per leaf explant (15.33 ± 1.45) after 28 d of continuous culture. The highest numbers of embryos (12.33 ± 0.88) were matured into plantlets in MS basal medium augmented with 0.27 μM BA and 58.4 mM sucrose. Histology showed the presence of scutellum, coleoptile, well-developed root, and shoot initials at different stages of somatic embryo (SE) development directly on leaf explants. About 85% of the plantlets were successfully acclimatized, and of these, 80.3% plants survived after secondary hardening under greenhouse conditions. The embryo-derived plants successfully flowered. The presence of monomorphic DNA bands in random amplified polymorphic DNA (RAPD) marker analysis confirmed the genetic homogeneity of direct somatic embryo-derived plantlets in respect to their mother plant.

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References

  • Beyramizade E, Azadi P, Mii M (2008) Optimization of factors affecting organogenesis and somatic embryogenesis of Anthurium andraeanum Lind. ‘Tera’. Propaga Ornamental Plants 8:198–203

    Google Scholar 

  • Borchetia S, Das SC, Handique PJ, Das S (2009) High multiplication frequency and genetic stability for commercialization of the three varieties of micropropagated tea plants (Camellia spp.). Sci Hortic 120:544–550

    Article  CAS  Google Scholar 

  • Dam A, Paul S, Bandyopadhyay TK (2010) Direct somatic embryogenesis and plant regeneration from leaf explants of Limonium sinensis (Girard) Kuntze. Sci Hortic 126:253–260

    Article  CAS  Google Scholar 

  • Dam A, Paul S, Bhattacharya C, Bandyopadhyay TK (2013) Effects of culture conditions on multiple shoot induction from inflorescence and RAPD analysis of cloned plants in Limonium sinensis (Girard) Kuntze, var. Golden Diamond. Plant Biochem Biotechnol 22:348–352

    Article  Google Scholar 

  • Denchev P, Velcheva M, Atanassov A (1991) A new approach to direct somatic embryogenesis in Medicago. Plant Cell Rep 10:338–341

    Article  CAS  PubMed  Google Scholar 

  • Doyle JJ, Doyle JL (1990) A rapid DNA isolation procedure for small quantities of fresh leaf tissue. Phytochem Bull 19:11–15

    Google Scholar 

  • Fehér A (2005) Why somatic plant cells start to form embryos? In: Mujib A, Samaj J (eds) Plant cell monographs (2). Somatic embryogenesis. Springer-Verlag Berlin, Heidelberg, pp 85–101

    Google Scholar 

  • Finnie JF, van Staden J (1986) In vitro culture of Anthurium andraeanum. S Afr J Bot 52:343–346

    Article  Google Scholar 

  • Gantait S, Sinniah UR, Mandal N, Das PK (2012) Direct induction of protocorm-like bodies from shoot tips, plantlet formation, and clonal fidelity analysis in Anthurium andreanum cv. Can. Plant Growth Regul 67:257–270

    Article  CAS  Google Scholar 

  • Geier T (1986) Factors affecting plant regeneration from leaf segments of Anthurium scherzerianum Schott (Araceae) cultured in vitro. Plant Cell Tissue Organ Cult 6:115–125

    Article  CAS  Google Scholar 

  • Gow WP, Chen JT, Chang WC (2010) Enhancement of direct somatic embryogenesis and plantlet growth from leaf explants of Phalaenopsis by adjusting culture period and explant length. Acta Physiol Plant 32:621–627

    Article  Google Scholar 

  • Kubeš M, Drážná N, Konrádová H, Lipavská H (2014) Robust carbohydrate dynamics based on sucrose resynthesis in developing Norway spruce somatic embryos at variable sugar supply. In Vitro Cell Dev Biol Plant 50:45–57

    Article  Google Scholar 

  • Kuehnle AR, Chen FC, Sugii N (1992) Somatic embryogenesis and plant regeneration in Anthurium andraeanum hybrids. Plant Cell Rep 11:438–442

  • Kuehnle AR, Sugii N (1991) Callus induction and plantlet regeneration in tissue cultures of Hawaiian Anthuriums. HortSci 26:919–921

    Google Scholar 

  • Kumari S, Desai JR, Shah RR (2011) Callus mediated plant regeneration of two cutflower cultivars of Anthurium andraeanum Hort. J Appl Hortic 13:37–41

    Google Scholar 

  • Kunisaki JT (1980) In vitro propagation of Anthurium andraeanum Lind. HortSci 15:508–509

    Google Scholar 

  • Larkin PJ, Scowcroft WR (1981) Somaclonal variation—a novel source of variability from cell cultures for plant improvement. Theor Appl Genet 60:197–214

    Article  CAS  PubMed  Google Scholar 

  • Martin KP, Joseph D, Madassey J, Philip VJ (2003) Direct shoot regeneration from lamina explants of two commercial cut flower cultivars of Anthurium andreanum Hort. In Vitro Cell Dev Biol Plant 39:500–504

    Article  Google Scholar 

  • Matsumoto TK, Kuehnle AR (1997) Micropropagation of Anthurium. In: Bajaj YPS (ed) High tech and micropropagation VI. Biotechnology in agriculture and forestry. Springer Verlag, Berlin Heidelberg, pp 14–29

    Google Scholar 

  • Matsumoto TK, Webb DT, Kuehnle AR (1996) Histology and origin of somatic embryos derived from Anthurium andraeanum Linden ex André Lamina. J Am Soc Hortic Sci 121:404–407

    Google Scholar 

  • Matsumoto TK, Kuehnle AR, Webb DT (1998) Zygotic embryogenesis in Anthurium (Araceae). Am J Bot 85:560–568

    Article  Google Scholar 

  • Mordhorst AP, Toonen MAJ, de Vries SC (1997) Plant embryogenesis. Crit Rev Plant Sci 16:535–576

    Article  Google Scholar 

  • Mujib A, Banerjee S, Maqsood M, Ghosh PD (2013) Somatic embryogenesis of some member ornamental genera of Amaryllidaceae and allied families: the similarities and differences. Open Hort J 6:9–18

    Article  CAS  Google Scholar 

  • Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassay with tobacco tissue cultures. Physiol Plant 15:473–497

    Article  CAS  Google Scholar 

  • Paul S, Dam A, Bhattacharyya A, Bandyopadhyay TK (2011) An efficient regeneration system via direct and indirect somatic embryogenesis for the medicinal tree Murraya koenigii. Plant Cell Tissue Organ Cult 105:271–283

    Article  Google Scholar 

  • Pedroso CM, Pais MS (1995) Factors controlling somatic embryogenesis. Plant Cell Tissue Organ Cult 43:147–154

    Article  Google Scholar 

  • Pierik RLM, Steegmans HHM, Van Der Meys JAJ (1974) Plantlet formation in callus tissue of Anthurium andraeanum Lind. Sci Hortic 2:193–198

    Article  Google Scholar 

  • Pinheiro MVM, Martins FB, da Cruz ACF, de Carvalho ACPP, Ventrella MC, Otoni WC (2013) Maturation of Anthurium andraeanum cv. Eidibel somatic embryos from nodal segments. In Vitro Cell Dev Biol-Plant 49:304–312

    Article  Google Scholar 

  • Pinheiro MVM, Martins FB, da Cruz ACF, de Carvalho ACPP, Ventrella MC, Otoni WC (2014) Somatic embryogenesis in anthurium (Anthurium andraeanum cv. Eidibel) as affected by different explants. Acta Sci Agron 36:87–98

    Article  Google Scholar 

  • Punyarani K, Devi KD, Singh CH, Singh NS, Singh HH, Singh TD, Moirangthem S, Devi HS (2013) In vitro production of genetically stable and virus free plantlets of Musa sp. var. Meitei Hei using male inflorescence as explant. Sci Hortic 164:440–447

    Article  CAS  Google Scholar 

  • Rout GR, Das P, Goel S, Raina SN (1998) Determination of genetic stability of micropropagated plants of ginger using random amplified polymorphic DNA (RAPD) markers. Bot Bull Acad Sin 39:23–27

    CAS  Google Scholar 

  • Skirvin RM, Mcpheeters KD, Norton M (1994) Sources and frequency of somaclonal variation. HortSci 29:1232–1237

    Google Scholar 

  • Smulders MJM, de Klerk GJ (2011) Epigenetics in plant tissue culture. Plant Growth Regul 63:137–146

    Article  CAS  Google Scholar 

  • Teixeira da Silva JA, Dobránszkib J, Winarto B, Zeng S (2015) Anthurium in vitro: a review. Sci Hortic 186:266–298

    Article  Google Scholar 

  • Vasil V, Hauptmann RM, Morrish FM, Vasil IK (1988) Comparative analysis of free DNA delivery and expression into protoplasts of Panicum maximum Jacq. (Guinea grass) by electroporation and polyethylene glycol. Plant Cell Rep 7:499–503

    Article  CAS  PubMed  Google Scholar 

  • Viégas J, Rocha MTR, Ferreira-Moura I, Rosa DL, Souza JA, Corrêa MGS, Silva JAT (2007) Anthurium andraeanum (Linden ex André) culture: in vitro and ex vitro. Floric Ornam Biotechnol 1:61–65

    Google Scholar 

  • Winarto B, da Silva JAT (2012) Influence of isolation technique of half-anthers and of initiation culture medium on callus induction and regeneration in Anthurium andreanum. Plant Cell Tissue Organ Cult 110:401–411

    Article  Google Scholar 

  • Winarto B, Rachmawati F, Pramanik D, Teixeira da Silva J (2011a) Morphological and cytological diversity of regenerants derived from half-anther cultures of anthurium. Plant Cell Tissue Organ Cult 105:363–374

    Article  Google Scholar 

  • Winarto B, Rachmawati F, Teixeira da Silva J (2011b) New basal media for half-anther culture of Anthurium andreanum Linden ex André cv. Tropical. Plant Growth Regul 65:513–529

    Article  CAS  Google Scholar 

  • Zhang Q, Chen J, Henny RJ (2006) Regeneration of Syngonium podophyllum ‘Variegatum’ through direct somatic embryogenesis. Plant Cell Tissue Organ Cult 84:181–188

    Article  CAS  Google Scholar 

  • Zhao J, Cui J, Liu J, Liao F, Henny RJ, Chen J (2012) Direct somatic embryogenesis from leaf and petiole explants of Spathiphyllum ‘Supreme’ and analysis of regenerants using flow cytometry. Plant Cell Tissue Organ Cult 110:239–249

    Article  Google Scholar 

Download references

Acknowledgments

The financial assistance from DST-PURSE Programme, University of Kalyani, and Personal Research Grants (PRGs) for teachers provided by the University of Kalyani, Kalyani, West Bengal, India, are gratefully acknowledged.

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Authors and Affiliations

  1. Department of Molecular Biology and Biotechnology, Faculty of Science, University of Kalyani, Kalyani, West Bengal, 741235, India

    Chayanika Bhattacharya, Anandamoy Dam, Joydeep Karmakar & Tapas Kumar Bandyopadhyay

Authors
  1. Chayanika Bhattacharya
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  2. Anandamoy Dam
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  3. Joydeep Karmakar
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  4. Tapas Kumar Bandyopadhyay
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Correspondence to Tapas Kumar Bandyopadhyay.

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Editor: Ewen Mullins

An erratum to this article can be found at http://dx.doi.org/10.1007/s11627-016-9777-2.

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Bhattacharya, C., Dam, A., Karmakar, J. et al. Direct somatic embryogenesis and genetic homogeneity assessment of regenerated plants of Anthurium andraeanum Linden cv. Fantasia. In Vitro Cell.Dev.Biol.-Plant 52, 512–519 (2016). https://doi.org/10.1007/s11627-016-9763-8

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  • DOI: https://doi.org/10.1007/s11627-016-9763-8

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