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Maturation of Anthurium andraeanum cv. Eidibel somatic embryos from nodal segments

  • Developmental Biology/Morphogenesis
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Abstract

Maturation of somatic embryos of Anthurium andraeanum cv. Eidibel from embryogenic callus was evaluated. Following induction of embryogenic calli from nodal segments, tissues were transferred to 125-mL Erlenmeyer flasks containing 25 mL liquid medium, with 0, 4.52, or 9.05 μM 2,4-dichlorophenoxyacetic acid and 0, 0.47, or 2.32 μM kinetin. Callus cultures were maintained in a dark growth room at 25 ± 2°C. At 45 d, the mass of embryogenic calli, number of primary and secondary somatic embryos, and percentage browning were evaluated. Nonparametric tests were used to evaluate color, texture, and somatic embryo development. The highest yield of somatic embryos was in the medium with 0.47 μM kinetin. Calli were friable, with a lower yield of secondary somatic embryos, and have minimal browning. Histology revealed polar globular somatic embryos and mature somatic embryos with defined apical and root meristematic zones, axillary buds, and primary leaves. These are important features for converting somatic embryos into plantlets.

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Acknowledgments

The authors thank the Fundação de Amparo à Pesquisa do Estado de Minas Gerais, Belo Horizonte, MG, Brazil (FAPEMIG grant number CRA-APQ-01451-12) for financial support, Coordenação de Aperfeiçoamento de Pessoal de Ensino Superior, Brasília, DF, Brazil for a research scholarship to M.V.M.P., and the IAC (Campinas, SP, Brazil) for kindly providing the in vitro clones of A. andraeanum cv. Eidibel (IAC 0-11) used in this work.

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Authors and Affiliations

  1. Departamento de Biologia Vegetal, Universidade Federal de Viçosa, 36570-000, Viçosa, MG, Brazil

    Marcos Vinícius Marques Pinheiro, Ana Claudia Ferreira da Cruz, Marília Contim Ventrella & Wagner Campos Otoni

  2. Instituto de Recursos Naturais, UNIFEI, Campus Prof. José Rodrigues Seabra, 37500-903, Itajubá, MG, Brazil

    Fabrina Bolzan Martins

  3. Embrapa Agroindústria Tropical, Rua Dra. Sara Mesquita, n° 2270, Bairro Pici, 60511-110, Fortaleza, CE, Brazil

    Ana Cristina Portugal Pinto de Carvalho

  4. Plant Biology Department, Plant Tissue Culture Laboratory/BIOAGRO, Federal University of Viçosa, University Campus, P.H. Rolfs Avenue, 36570-000, Viçosa, MG, Brazil

    Wagner Campos Otoni

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  1. Marcos Vinícius Marques Pinheiro
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  2. Fabrina Bolzan Martins
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  3. Ana Claudia Ferreira da Cruz
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  4. Ana Cristina Portugal Pinto de Carvalho
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  5. Marília Contim Ventrella
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  6. Wagner Campos Otoni
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Correspondence to Wagner Campos Otoni.

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Pinheiro, M.V.M., Martins, F.B., da Cruz, A.C.F. et al. Maturation of Anthurium andraeanum cv. Eidibel somatic embryos from nodal segments. In Vitro Cell.Dev.Biol.-Plant 49, 304–312 (2013). https://doi.org/10.1007/s11627-013-9522-z

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