Abstract
Somatic embryogenesis is a unique method of in vitro regeneration, which can be used in plant reproduction, germplasm conservation, and molecular-assisted breeding. The results showed that the optimum medium for embryogenic callus induction was MS + 6 mg L−1 6-BA + 1.5 mg L−1 TDZ + 0.5 mg L−1 NAA + 30 g L−1 sucrose + 7 g L−1 agar, and the induction rate was 47.45%. The best somatic differentiation medium was MS + 2 mg L−16-BA + 1.5 mg L−1 TDZ + 30 g L−1 sucrose + 7 g L−1 agar, and the induction rate of somatic embryos was 54.45%. The optimum medium for embryoid proliferation was MS + 6 mg L−1 6-BA + 1 mg L−1 NAA + 0.2 mg L−1 TDZ, and the proliferation rate and the multiplication coefficient reached 46.33% and 7.83, respectively. The mature somatic embryos were put into MS, B5, and 1/2MS medium for seedling culture. In MS medium, true leaves grew, complete plants were obtained, and the seedling rate was 88.00%. At the same time, the survival rate of transplanting seedlings in the mixed matrix (peat: organic fertilizer: soil = 1:1:1) was as high as 98%. Cytological observation showed that the somatic embryos underwent globular, heart-shaped, torpedo, and cotyledon stages. This study established a regeneration system of C. × generalis with excellent somatic embryos, and provided basic technical support for the large-scale commercial propagation and germplasm resources protection. It will lay a foundation for further research on gene function and breeding new varieties and ideal research materials for the study of somatic embryogenesis mechanism and genetic transformation of C. × generalis.
Key Message
Somatic embryos was acquired successfully from the embryogenic callus of C. × generalis, cytological observation showed that the somatic embryos underwent globular, heart-shaped, torpedo, and cotyledon stages. Histological analysis confirmed the vascular bundle separation between embryoid and maternal tissue.
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Application and industrialization project of scientific and technological achievements in Guizhou Province (2021 General 009) and National Natural Science Foundation of China (31460371).
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All authors contributed to the study conception. Material preparation was performed by ZYG, FY and GFW. Data collection was accomplished by ZYG and MLS. Data analysis, experiment design and supervision were performed by XJP, WEZ and FY. The draft of the manuscript was written by ZYG and WEZ revised the article. All authors commented on previous versions of the manuscript and all authors read and approved the final manuscript.
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Communicated by Yan Liu.
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Gan, Zy., Shu, Ml., Yang, F. et al. Somatic embryo induction and plantlet regeneration of Canna × generalis from immature zygotic embryo. Plant Cell Tiss Organ Cult 155, 681–692 (2023). https://doi.org/10.1007/s11240-023-02588-2
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DOI: https://doi.org/10.1007/s11240-023-02588-2