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A highly efficient method for somatic embryogenesis of Kelussia odorotissima Mozaff., an endangered medicinal plant

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Abstract

Kelussia odoratissima Mozaff. (or Kelus) is a medicinal plant native to the Zagros Mountains in Iran. This plant is widely used as a food flavoring and for its health-promoting properties. It has been considered an endangered species by the United Nations Development Programme. In this study, a somatic embryogenesis (SE) method was developed for mass propagation of Kelus. The green globular embryogenic callus was induced on cotyledonary leaves using the Murashige and Skoog (MS) medium supplemented with 1 mg/l 2,4-dichlorophenoxyaceticacid (2,4-D) and 0.25 mg/l Kinetin. Different treatments were assayed for proliferation of the embryogenic callus. The calli remained embryogenic in an MS medium containing 2,4-D (1 mg/l). The light treatments and carbon source showed significant effects (P ≤ 0.05) on the proliferation and development of somatic embryos. These treatments improved the conversion rate of the cotyledonary-stage embryos by 100%. The average numbers of embryos in the globular, heart, torpedo, and cotyledonary stages decreased by the addition of 3 g/l case in hydrolisate. The genetic stability among tissue culture-derived plants and the mother plant were assessed using the amplification fragment length polymorphism. No polymorphic band was observed among all the plants, exhibiting the genetic stability during in vitro multiplication. This research provides a promising approach for true-to-type plant multiplication of K. odoratissima through SE.

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Acknowledgements

The project was supported a grant from the Agricultural Biotechnology Research Institute of Iran.

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Authors and Affiliations

  1. Department of Plant Tissue Culture, Agricultural Biotechnology Research Institute of Iran-Isfahan Branch, Agricultural Research, Education and Extension Organization (AREEO), Tehran, Iran

    Mortaza Ebrahimi & Arash Mokhtari

  2. Department of Genomics, Agricultural Biotechnology Research Institute of Iran-Isfahan Branch, Agricultural Research, Education and Extension Organization (AREEO), Tehran, Iran

    Rasoul Amirian

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  1. Mortaza Ebrahimi
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  2. Arash Mokhtari
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  3. Rasoul Amirian
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ME is the project leader and this manuscript is a part of the results of a research project on Kelussiaodoratissima carried out at the ABRII-Isfahan Branch. AM and RA cooperated in the research.

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Correspondence to Mortaza Ebrahimi.

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Communicated by Sergio J Ochatt.

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Ebrahimi, M., Mokhtari, A. & Amirian, R. A highly efficient method for somatic embryogenesis of Kelussia odorotissima Mozaff., an endangered medicinal plant. Plant Cell Tiss Organ Cult 132, 99–110 (2018). https://doi.org/10.1007/s11240-017-1314-4

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