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Genetic stability of micropropagated almond plantlets, as assessed by RAPD and ISSR markers

  • Genetics and Genomics
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Abstract

Almond shoots produced by axillary branching from clone VII derived from a seedling of cultivar Boa Casta were evaluated for somaclonal variation using randomly amplified polymorphic DNA (RAPD) and inter-simple sequence repeats (ISSR) analysis. To verify genetic stability we compared RAPD and ISSR patterns of plantlets obtained after 4 and 6 years of in vitro multiplication. A total of 64 RAPD and 10 ISSR primers gave 326 distinct and reproducible band classes, monomorphic across all 22 plantlets analysed. Thus, a total of 7,172 bands were generated, exhibiting homogeneous RAPD and ISSR patterns for the plantlets tested. These results suggest that the culture conditions used for axillary branching proliferation are appropriate for clonal propagation of almond clone VII, as they do not seem to interfere with the integrity of the regenerated plantlets. These results allowed us to establish the use of axillary branching plantlets (mother-plants) as internal controls for the analysis of somaclonal variation of shoots regenerated from other in vitro culture processes performed with clone VII (adventitious regeneration, regeneration from meristem culture, virus sanitation programs and genetic engineering).

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Acknowledgements

Part of this research was supported by Praxis XXI through the PhD grant BD/11323/97 provided to M.C. Martins and the research projects PRAXIS 3/3.2/HORT/2143/95. We also gratefully acknowledge Dr. Célia Miguel and Milene Costa, for providing the in vitro plant material.

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Authors and Affiliations

  1. IBET/ITQB, Quinta do Marquês, 2784-505, Oeiras, Portugal

    M. Martins, D. Sarmento & M. M. Oliveira

  2. Faculdade de Ciências, Dep. Biologia Vegetal, Universidade de Lisboa, Campo Grande, 1749-016, Lisboa, Portugal

    M. Martins & M. M. Oliveira

Authors
  1. M. Martins
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  2. D. Sarmento
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  3. M. M. Oliveira
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Corresponding author

Correspondence to M. M. Oliveira.

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Communicated by P. Puigdoménech

M. Martins and D. Sarmento contributed equally to this paper

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Martins, M., Sarmento, D. & Oliveira, M.M. Genetic stability of micropropagated almond plantlets, as assessed by RAPD and ISSR markers. Plant Cell Rep 23, 492–496 (2004). https://doi.org/10.1007/s00299-004-0870-3

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  • DOI: https://doi.org/10.1007/s00299-004-0870-3

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