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Observations on the combined effects of light, NAA and 2,4-D on somatic embryogenesis of cucumber (Cucumis sativus) hybrids

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Abstract

Somatic embryogenesis of cucumber was affected by auxin and light during the induction phase. In the light, 2,4-dichlorophenoxyacetic acid (2,4-D) alone induced little embryogenesis, while combined with naphthaleneacetic acid (NAA) it induced 1.25 somatic embryos (SEs) per callus. In the dark 2,4-D alone induced 5 times more SEs per callus.

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Abbreviations

2,4-D:

2,4-Dichlorophenoxyacetic acid

NAA:

Naphthaleneacetic acid

M&S:

Murashige and Skoog (1962)

PGR:

Plant, growth, regulator

SE:

Somatic embryo

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Authors and Affiliations

  1. Department of Agricultural & Water Research, Plant Tissue Culture Laboratory, Doha, Qatar

    Khaled M. Suliman Elmeer

  2. School of Biology & Environmental Sciences, UCD-Dublin, Belfield, Dublin 4, Ireland

    Michael J. Hennerty

Authors
  1. Khaled M. Suliman Elmeer
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  2. Michael J. Hennerty
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Correspondence to Khaled M. Suliman Elmeer.

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Elmeer, K.M.S., Hennerty, M.J. Observations on the combined effects of light, NAA and 2,4-D on somatic embryogenesis of cucumber (Cucumis sativus) hybrids. Plant Cell Tiss Organ Cult 95, 381–384 (2008). https://doi.org/10.1007/s11240-008-9439-0

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  • DOI: https://doi.org/10.1007/s11240-008-9439-0

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